1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22317-5 SANS 5302:2009Edition 3SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the skin and hide beetle (Dermestes macu
4、latus De Geer) Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5302:2009 Edition 3 Table of changes Change No. Date Scope Foreword This South African standard was approved by National C
5、ommittee SABS SC 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in February 2009. This document supersedes SABS SM 302:197
6、7 (first revision). SANS 5302:2009 Edition 3 1 Pesticides Rearing and handling of the skin and hide beetle (Dermestes maculatus De Geer) 1 Scope This standard specifies a method for the rearing and handling of the skin and hide beetle (Dermestes maculatus De Geer). 2 Normative references The followi
7、ng referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international st
8、andards can be obtained from the SABS Standards Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. SANS 3310-1/ISO 3310-1, Test sieves Technical requirements and testing Part 1: Test sieves of metal wire cloth. 3 Rearing conditions Maintain
9、 the rearing conditions prescribed in SANS 5808. 4 Rearing equipment 4.1 Brush, soft hairbrush. 4.2 Rearing containers and lids, type A rearing containers and type A rearing container lids as described in SANS 5808, or any other suitable round, square or rectangular containers. 4.3 Sand, sieved (see
10、 4.4) and sterilized by heating at 70 C for at least 1 h or until dry. 4.4 Sieve, type A sieve as described in SANS 5808 of nominal aperture size 1,7 mm, and that complies with the relevant requirements of SANS 3310-1. 5 Media 5.1 Prepare oviposition and rearing medium as follows: a) Cut unbruised u
11、nsalted beef that is free from sinew and fat into strips approximately 50 mm wide and 40 mm thick and of convenient length. Dry the strips before a rapidly revolving fan until the meat is dry and hard on the outside but still moist on the inside. SANS 5302:2009 Edition 3 2 b) Divide a tin of canned
12、corned beef into four pieces and use two of the pieces at a time. 5.2 Saturate pieces of sterile cotton wool with tap water and place in disposable plastics containers of approximate height 60 mm and diameter 110 mm. 6 Procedure 6.1 Place approximately 250 g of the oviposition and rearing medium (se
13、e 5.1) and a water container (see 5.2) on a 20 mm thick layer of sand (see 4.3) in a rearing container (see 4.2). 6.2 Place in the rearing container approximately 600 adult skin and hide beetles. NOTE The females will deposit their eggs on the medium and the sand. 6.3 Remove the medium after one wee
14、k, shaking off the beetles by sharply tapping the meat against the side of the rearing container. 6.4 Sift the contents of the rearing container through the sieve (see 4.4), and return the sand to the container. The adults and coarse debris will be retained on the sieve. 6.5 Separate the adults from
15、 the debris using the brush (see 4.1) and retain them for use in 6.8. 6.6 Place the medium containing the eggs (see 6.3) and a fresh water container on the sand in the rearing container (see 6.4). 6.7 Examine the rearing container regularly and replenish with medium and fresh saturated cotton wool a
16、s and when required. The adults will emerge after approximately eight weeks. 6.8 Rear new colonies by repeating the procedure in 6.1 to 6.7 (inclusive), but using for each colony 300 of the adults retained in 6.5 and 300 of the freshly-emerged adults (see 6.7). 6.9 Destroy the excess adults from 6.5
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