1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22075-4 SANS 5458:2008Edition 4SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the German cockroach (Blatella germanica
4、 (L.) Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5458:2008 Edition 4 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committee
5、SABS SC 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in December 2008. This document supersedes SABS SM 458:1977 (second
6、 revision). SANS 5458:2008 Edition 4 1 Pesticides Rearing and handling of the German cockroach (Blatella germanica (L.) 1 Scope This standard specifies a method for the rearing and handling of the German cockroach (Blatella germanica (L.). 2 Normative references The following referenced documents ar
7、e indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obtained fr
8、om the SABS Standards Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. 3 Rearing conditions Maintain the rearing conditions prescribed in SANS 5808. 4 Rearing equipment 4.1 Feeding dishes, type B plastics dishes as described in SANS 5808.
9、 4.2 Rearing containers and lids, type B rearing containers and type A rearing container lids as described in SANS 5808. 4.3 Shelters, cockroach shelters as described in SANS 5808. 4.4 Sieve, type A sieve as described in SANS 5808 of nominal aperture size 4,0 mm. 5 Rearing media 5.1 Finely ground do
10、g biscuits contained in a feeding dish (see 4.1). 5.2 Cotton wool saturated with water that contains methyl-4-hydroxy benzoate (0,9 g/L), and placed in a feeding dish (see 4.1). SANS 5458:2008 Edition 4 2 6 Procedure 6.1 Place about 2 000 (20 mL of) freshly-emerged first nymphal instars of the Germa
11、n cockroach (see 6.6) in a clean rearing container (see 4.2) and supply with the rearing media (see 5.1 and 5.2) and the shelters (see 4.3) arranged in two tiers (not more than 20 shelters per rearing container). 6.2 After 12 weeks, anaesthetize the insects in the rearing container using carbon diox
12、ide, and separate the females with othecae, the females without othecae, the males, and the nymphs manually. Destroy any nymphs found at this stage. 6.3 Return females without othecae to the rearing container and examine them after one week for the development of othecae. Destroy females still witho
13、ut othecae, and transfer the others to the rearing container described in 6.5. 6.4 Place males in a separate rearing container and supply with the rearing media and the shelters. NOTE Only males are used for the evaluation of pesticides. 6.5 Place females with othecae (see 6.2) in a clean rearing co
14、ntainer and supply with the rearing media and the shelters. 6.6 After one week, and each consecutive week thereafter, anaesthetize the adult breeding stock (see 6.5) in the rearing container using carbon dioxide, and use the sieve (see 4.4) to separate the adults from the first nymphal instars. Repe
15、at this procedure until all the adults have died. 6.7 After the nymphs and adults are separated (see 6.6), discard any dead adults and return the others to the rearing container. Supply with fresh rearing media. 6.8 Supply the colony with fresh rearing medium once every week. NOTE If the colony should become too large to be contained in one rearing container, the insects may be distributed over two or more containers. 6.9 Rear new colonies by repeating the procedure in 6.1 to 6.8 (inclusive) for each colony. SABS
