1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22076-1 SANS 5459:2008Edition 4SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the confused flour beetle (Tribolium con
4、fusum (Duv.) Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5459:2008 Edition 4 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Com
5、mittee SABS SC 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in December 2008. This document supersedes SABS SM 459:1978
6、(second revision). SANS 5459:2008 Edition 4 1 Pesticides Rearing and handling of the confused flour beetle (Tribolium confusum (Duv.) 1 Scope This standard specifies a method for the rearing and handling of the confused flour beetle (Tribolium confusum (Duv.). 2 Normative references The following re
7、ferenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standar
8、ds can be obtained from the SABS Standards Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. 3 Rearing conditions Maintain the rearing conditions prescribed in SANS 5808. 4 Rearing equipment 4.1 Rearing containers and lids, type A rearing
9、containers and type A rearing container lids as described in SANS 5808. 4.2 Sieve, type A sieve as described in SANS 5808 of nominal aperture size 850 m. 5 Rearing medium A mixture of the following: a) 1 125 g cake flour, heat-sterilized at a temperature of 60 C 5 C for 30 min; b) 1 125 g coarse whe
10、at flour, obtained by sifting ground wheat through the sieve (see 4.2) and heat-sterilizing the fine fraction at a temperature of 60 C 5 C for 30 min; c) 270 g full-cream milk powder; and d) 180 g fishmeal, sifted (see 4.2) and heat-sterilized at a temperature of 60 C 5 C for 30 min. SANS 5459:2008
11、Edition 4 2 6 Procedure 6.1 Place 100 mL of adult flour beetles (about 16 000 beetles) on top of the rearing medium (see clause 5) contained in a rearing container (see 4.1). 6.2 After 14 d separate the rearing medium and eggs from the adults by sieving the entire contents of the rearing container t
12、hrough the sieve (see 4.2). Retain the adults that remain behind on the sieve for use in 6.5. 6.3 Transfer the rearing medium and eggs to a fresh rearing container. 6.4 The adult stage is reached after approximately 8 weeks to 10 weeks and adults are separated by sieving as in 6.2. 6.5 Start a new colony by repeating the procedure in 6.1 to 6.4 (inclusive), but using 50 mL of the flour beetles retained in 6.2 and 50 mL of the flour beetles obtained in 6.4. SABS
