2018_2019学年高中生物专题1课题3制作泡菜并检测亚硝酸盐含量课件新人教版选修1.ppt

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1、室温,1518 ,3035 ,1825 ,适宜温度,异养厌氧,异养需氧,异养需氧,异养兼性厌氧,代谢类型,原核生物,真核生物,原核生物,真核生物,分类,乳酸菌,毛霉,醋酸菌,酵母菌,微生物,制作酸奶、泡菜,制作 腐乳,酿醋,酿酒、发面,生产应用,二分裂 生殖,孢子 生殖,二分裂生殖,孢子生殖,适宜条件下进行出芽生殖,生殖方式,无氧,一直 需氧,一直 需氧,前期需氧;后期不需氧,发酵条件,乳酸菌,毛霉,醋酸菌,酵母菌,微生物,制备相应浓度的标准显色液,干燥后的亚硝酸钠,5,干燥剂,用于干燥亚硝酸钠,硅胶,4,与重氮化反应的产物结合,生成玫瑰红色染料,作为测定亚硝酸盐含量的指示剂,N1萘基 乙二胺

2、盐酸盐,3,调节pH成酸性,盐酸,2,与亚硝酸盐发生重氮化反应,对氨基苯磺酸,1,作用,试剂、药品,序号,作为溶剂,蒸馏水,9,作为吸附剂,使泡菜滤液脱色,变澄清,氢氧化铝,8,中和过多的盐酸,营造碱性环境,氢氧化钠,7,溶于蒸馏水并用浓盐酸酸化后作为亚硝酸盐的提取剂,氯化镉、氯化钡,6,作用,试剂、药品,序号,下降至相对稳定(硝酸盐还原菌被完全抑制),继续增多,pH继续下降,减少(乳酸继续积累,pH继续下降,抑制其活动),发酵后期,下降(硝酸盐还原菌受抑制,部分亚硝酸盐被分解),积累、增多、pH下降,最多(乳酸抑制其他菌活动),发酵中期,增加(硝酸盐还原菌的作用),少,少(有O2,乳酸菌活动受抑制),发酵初期,亚硝酸盐,乳酸,乳酸菌,发酵时期,亚硝酸盐是硝酸盐还原菌促进硝酸盐还原形成的,而不是硝化细菌氧化氨形成的,注意,含量变化曲线,亚硝酸盐,乳酸,乳酸菌,发酵时期,0.20,0.05,0.10,14 d,0.20,0.05,0.10,10 d,0.60,0.10,0.20,7 d,0.80,0.20,0.60,4 d,0.15,0.15,0.15,封坛前,3号坛,2号坛,1号坛,亚硝酸盐含量腌制天数,坛号,

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