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2018_2019学年高中生物专题5课题2多聚酶链式反应扩增DNA片段课件新人教版选修1.ppt

上传人：王申宇 文档编号：1121263 上传时间：2019-05-04 格式：PPT 页数：40 大小：2.89MB
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RNA或单链DNA分子片段,一小段RNA,引物,耐高温的DNA聚合酶(Taq DNA聚合酶),解旋酶、DNA聚合酶,酶,加热至90 以上时，双链全部解开,在解旋酶的作用下，边解旋边复制,解旋,不 同 点,PCR反应,体内DNA复制,都需提供DNA复制的模板 都需要四种脱氧核苷酸原料 都需要分别与两条模板链相结合的两种引物 子链延伸的方向都是从5端到3端,相同点,高温,体内温和条件,温度,两条子链都是连续合成,一条子链连续合成，另一条子链不连续合成,合成子链,不 同 点,PCR反应,体内DNA复制,

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