1、BSI Standards Publication WB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06 Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties BS ISO 8255-1:2017 ISO 2017 Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties Microsc
2、opes Lamelles couvre-objet Partie 1: Tolrances dimensionnelles, paisseur et proprits optiques INTERNATIONAL STANDARD ISO 8255-1 Third edition 2017-06 Reference number ISO 8255-1:2017(E) National foreword This British Standard is the UK implementation of ISO 8255-1:2017. It supersedes BS ISO 8255-1:2
3、011, which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CPW/172, Optics and Photonics. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provi
4、sions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017 ISBN 978 0 580 96382 7 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was publish
5、ed under the authority of the Standards Policy and Strategy Committee on 30 June 2017. Amendments/corrigenda issued since publication Date Text affected BRITISH STANDARD BS ISO 8255-1:2017 ISO 2017 Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties Microscopes
6、 Lamelles couvre-objet Partie 1: Tolrances dimensionnelles, paisseur et proprits optiques INTERNATIONAL STANDARD ISO 8255-1 Third edition 2017-06 Reference number ISO 8255-1:2017(E) BS ISO 8255-1:2017 ISO 8255-1:2017(E)ii ISO 2017 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2017, Published
7、in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission c
8、an be requested from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyrightiso.org www.iso.org BS ISO 8255-1:2017 ISO 8255-1:2017(E)
9、Foreword iv Introduction v 1 Scope . 1 2 Normative references 1 3 T erms and definitions . 1 4 Requirements 1 4.1 General . 1 4.2 Dimensional tolerances for thickness . 1 4.3 Dimensional tolerances for length, width and diameter 2 4.4 Optical properties . 2 5 Marking and labelling . 3 Annex A (infor
10、mative) Guidelines for selection of cover glass type . 4 Annex B (informative) Typical dimensions for rectangular and round cover glasses 5 Bibliography 6 ISO 2017 All rights reserved iii Contents Page BS ISO 8255-1:2017 ISO 8255-1:2017(E) Foreword ISO (the International Organization for Standardiza
11、tion) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be
12、represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to
13、 develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of th
14、e ISO/IEC Directives, Part 2 (see www .iso .org/ directives). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified
15、 during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents). Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. For an explanation o
16、n the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: w w w . i s o .org/ is
17、o/ foreword .html. The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes. This third edition cancels and replaces the second edition (ISO 8255-1:2011), of which it constitutes a minor revision to correct the designation of cove
18、r glass. A list of all parts in the ISO 8255 series can be found on the ISO website.iv ISO 2017 All rights reserved BS ISO 8255-1:2017 ISO 8255-1:2017(E) Introduction This document defines dimensions and specifies optical quality requirements in order to guarantee the quality of observation. The dat
19、a given in this document are applicable to most products in use and have been adapted to take into account the relevant national standards in vigour. This document contains requirements for dimensional tolerances, thickness and optical properties, whereas quality requirements and test methods relate
20、d to the material are dealt with in ISO 8255-2. ISO 2017 All rights reserved v BS ISO 8255-1:2017BS ISO 8255-1:2017 Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties 1 Scope This document specifies requirements for dimensional tolerances, thickness and optica
21、l properties for microscope cover glasses used for transmitted light microscopy in the visible spectral range (400 nm to 760 nm). 2 Normative references The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For
22、dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 8036, Microscopes Immersion liquids for light microscopy 3 T erms a nd definiti ons No terms and definitions are listed in this document. IS
23、O and IEC maintain terminological databases for use in standardization at the following addresses: IEC Electropedia: available at h t t p :/ www .electropedia .org/ ISO Online browsing platform: available at h t t p :/ www .iso .org/ obp 4 Requirements 4.1 General All media that are located between
24、the specimen and the microscope objective are in their optical effect part of the objective. Such media are usually cover glasses and immersion media. Immersion media are defined in ISO 8036; their refractive index shall be taken into account for the selection of the cover glass. Microscope objectiv
25、es, unless equipped with correction collars, are designed for a specific immersion medium (e.g. air, oil or water) and cover glass thickness. The design thickness is t = 0,17 mm, unless otherwise marked on the objective. When using microscope objectives with high numerical aperture, deviations from
26、the nominal cover glass thickness leads to severe optical aberrations, mainly spherical aberration. The refractive index of the cover glass material needs to be specified for a broad spectral range to maintain good chromatic correction. This is achieved by specification of the refractive index, n e
27、, for a reference wavelength ( e= 546,07 nm) near the maximum of the eyes spectral sensitivity and the corresponding Abbe number, e . 4.2 Dimensional tolerances for thickness The thickness tolerances of cover glasses shall be in accordance with Table 1. INTERNATIONAL ST ANDARD ISO 8255-1:2017(E) ISO
28、 2017 All rights reserved 1 BS ISO 8255-1:2017 ISO 8255-1:2017(E) Table 1 Dimensional tolerances of types of cover glasses Dimensions in millimetres Designation/Type Design thickness t Tolerance No. 1 0,17 No. 1 H 0,17 No. 1 0,17 In addition to the thicknesses given in Table 1, cover glasses are ava
29、ilable in other thicknesses. These can be used for certain applications or in combination with objectives specifically designed for such non-standard cover glasses. However, it shall be pointed out that the highest optical quality may not necessarily be obtained with such cover glasses, when using o
30、bjectives of high numerical aperture. Annex A provides guidelines for the selection of cover glass No. 1 and No. 1 H with objective numerical aperture values in combination with common immersion media. 4.3 Dimensional tolerances for length, width and diameter The limiting tolerances for nominal leng
31、th, l, and width, w, for rectangular cover glasses (Form A) as shown in Figure 1 or diameter, d, for round cover glasses (Form B) as shown in Figure 2 are 0,5 mm. Typical dimensions for rectangular and round cover glasses are given in Table B.1. Figure 1 Form A: Rectangular cover glass Figure 2 Form
32、 B: Round cover glass 4.4 Optical properties Cover glasses shall have the following optical properties: Principal refractive index: n e= 1,525 5 0,001 5 Abbe number: e= 56 22 ISO 2017 All rights reserved BS ISO 8255-1:2017 ISO 8255-1:2017(E) NOTE The principal refractive index, n e , is the index of
33、 refraction for light of wavelength e= 546,07 nm (green Mercury e-line). This wavelength is located close to the maximum spectral sensitivity of the human eye and is commonly used as a reference wavelength for optical computations (see ISO 7944). The Abbe number, e , is computed according to Formula
34、 (1): (1) wheren e is the principal refractive index;n F is the refractive index for light of wavelength F= 479,99 nm (blue Cadmium F-line);n C is the refractive index for light of wavelength C= 643,85 nm (red Cadmium C-line). 5 Marking and labelling The packaging of microscope cover glasses that co
35、mply with this document shall display the following information: a) the thickness, No. 1 , No. 1 H, or No. 1; b) the dimensions for length and width, or diameter; c) the average number of cover glasses per package or mass; d) the name of the manufacturer or supplier and country of origin; e) a marki
36、ng to show that the cover glasses comply with the requirements of this document. ISO 2017 All rights reserved 3 BS ISO 8255-1:2017 ISO 8255-1:2017(E) Annex A (informative) Guidelines for selection of cover glass type To achieve optimum results, using the cover glass types shown in Table A .1 is reco
37、mmended. The proper selection of the cover glass type depends on the application and individual properties of the objective as shown in Table A .1. Users, including those with objectives with mechanisms to correct for cover glass thickness, should always use cover glass that conforms with the other
38、optical properties specified in this document. Table A.1 Guidelines for selection of cover glass type Immersion medium Min. numerical aperture Max. numerical aperture Type of cover glass Air 0,35 0,70 No. 1 Air 0,70 No. 1 H Water 0,60 0,90 No. 1 Water 0,90 No. 1 H Glycerol 0,80 1,10 No. 1 Glycerol 1
39、,10 No. 1 H Immersion oil 0,90 1,30 No. 1 Immersion oil 1,30 No. 1 H4 ISO 2017 All rights reserved BS ISO 8255-1:2017 ISO 8255-1:2017(E) Annex B (informative) Typical dimensions for rectangular and round cover glasses Table B.1 Typical dimensions for rectangular and round cover glasses Dimensions in
40、 millimetres Form Length l 0,5 Width w 0,5 Diameter d 0,5 A 18 18 22 22 24 32 40 50 60 24 B 18 22 ISO 2017 All rights reserved 5 BS ISO 8255-1:2017 ISO 8255-1:2017(E) Bibliography 1 ISO 7944, Optics and optical instruments Reference wavelengths6 ISO 2017 All rights reserved BS ISO 8255-1:2017BS ISO
41、8255-1:2017 ISO 8255-1:2017(E) ISO 2017 All rights reserved IC S 37.020 Price based on 6 pages BS ISO 8255-1:2017This page deliberately left blank ISO 8255-1:2017(E) ISO 2017 All rights reserved IC S 37.020 Price based on 6 pagesThis page deliberately left blank BSI is the national body responsible
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