1、BSI Standards Publication BS ISO 8255-2:2013 Microscopes Cover glasses Part 2: Quality of materials, standards of finish and mode of packagingBS ISO 8255-2:2013 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 8255-2:2013. It supersedes BS 7011-3.2:1998 which
2、is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CPW/172, Optics and Photonics. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a
3、contract. Users are responsible for its correct application. The British Standards Institution 2013. Published by BSI Standards Limited 2013 ISBN 978 0 580 81114 2 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under t
4、he authority of the Standards Policy and Strategy Committee on 30 September 2013. Amendments issued since publication Date Text affectedBS ISO 8255-2:2013 ISO 2013 Microscopes Cover glasses Part 2: Quality of materials, standards of finish and mode of packaging Microscopes Lamelles couvre-objet Part
5、ie 2: Qualit des matriaux, normes de finition et mode demballage INTERNATIONAL STANDARD ISO 8255-2 Second edition 2013-09-15 Reference number ISO 8255-2:2013(E)BS ISO 8255-2:2013ISO 8255-2:2013(E)ii ISO 2013 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2013 All rights reserved. Unless otherw
6、ise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address be
7、low or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in SwitzerlandBS ISO 8255-2:2013ISO 8255-2:2013(E) ISO 2013 All rights reserved iii Contents
8、Page Foreword iv Introduction v 1 Scope . 1 2 Normative references 1 3 Terms and definitions . 1 4 Requirements 2 4.1 Transparency and colour . 2 4.2 Non-flatness (waviness) and non-parallelism . 2 4.3 Durability . 3 4.4 Surface quality and inclusions . 3 4.5 Edge finish. 3 4.6 Cleanliness and cloud
9、iness . 3 4.7 Adhesion . 3 4.8 Residual stress/birefringence . 3 5 Sampling 3 5.1 General . 3 5.2 Quality measurement for cover glasses for conformance with this part of ISO 8255 . 3 5.3 Drawing of samples and units inspection 4 6 Test methods . 4 6.1 General . 4 6.2 Package suitability . 4 6.3 Adhe
10、sion . 5 6.4 Cleanliness and cloudiness . 5 6.5 Surface quality and inclusions . 5 6.6 Transparency and colour . 6 6.7 Edge finish. 6 6.8 Non-flatness (waviness) and non-parallelism . 6 6.9 Durability Chemical durability of surface and resistance to atmospheric attack (Solubility) . 6 6.10 Residual
11、stress/birefringence . 7 7 Marking/labelling . 7 8 Packaging . 7 Annex A (normative) Sample size code letters and single sampling plans for normal inspection .8 Bibliography .10BS ISO 8255-2:2013ISO 8255-2:2013(E) Foreword ISO (the International Organization for Standardization) is a worldwide feder
12、ation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that commit
13、tee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and
14、 those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part
15、 2. www.iso.org/directives. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the doc
16、ument will be in the Introduction and/or on the ISO list of patent declarations received. www.iso.org/patents. Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. The committee responsible for this document is ISO/TC 172, Opt
17、ics and Photonics, Subcommittee SC 5, Microscopes and endoscopes. This second edition cancels and replaces the first edition (ISO 8255-2:1997), of which it constitutes a minor revision. ISO 8255 consists of the following parts, under the general title Microscopes Cover glasses: Part 1: Dimensional t
18、olerances, thickness and optical properties Part 2: Quality of materials, standards of finish and mode of packagingiv ISO 2013 All rights reservedBS ISO 8255-2:2013ISO 8255-2:2013(E) Introduction The data given in this part of ISO 8255 are intended to provide for adequate performance of the product
19、for the end user. They are applicable to most products in use and have been adapted to take into account the relevant national standards in force. Dimensions and optical qualities are specified in ISO 8255-1. ISO 2013 All rights reserved vBS ISO 8255-2:2013BS ISO 8255-2:2013Microscopes Cover glasses
20、 Part 2: Quality of materials, standards of finish and mode of packaging 1 Scope This part of ISO 8255 specifies requirements and methods of test for the quality of material, standards of finish, and mode of packaging for microscope cover glasses. This part of ISO 8255 is applicable to microscope co
21、ver glasses for use in transmitted light microscopy (400 nm to 760 nm). 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated refere
22、nces, the latest edition of the referenced document (including any amendments) applies. ISO 2859-1:1999, Sampling procedures for inspection by attributes Part 1: Sampling schemes indexed by acceptance quality limit (AQL) for lot-by-lot inspection ISO 8255-1:2011, Dimensional tolerances, thickness, a
23、nd optical properties ISO 11455:1995, Raw optical glass Determination of birefringence 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 seed small bubbles in glass, sometimes elongated 3.2 cord vitreous compositional inhomogeneities in glass (
24、also known as striae, ream, or glassy knots) 3.3 line fine parallel line on glass surface in direction of draw 3.4 nick place where minute piece(s) of glass have been removed from edges of glass, giving rise to poor edge finish 3.5 cleanliness freedom from visible contamination such as fingerprints,
25、 particulate matter, or residue left from cleaning process INTERNATIONAL ST ANDARD ISO 8255-2:2013(E) ISO 2013 All rights reserved 1BS ISO 8255-2:2013ISO 8255-2:2013(E) 3.6 cloudiness haze light scattering or reduced transparency due to surface deterioration typically as a result of atmospheric atta
26、ck in the presence of humidity and CO 2 3.7 abrasion surface damage and pitting typically caused by vibration of one slide surface on another during packaging or during shipment and handling 3.8 acceptance quality limit AQL quality level that is the worst tolerable process average when a continuing
27、series of lots is submitted for acceptance sampling Note 1 to entry: This concept only applies when a sampling scheme with rules for switching and discontinuation, such as in ISO 2859-1 and in ISO 3951, is used. Note 2 to entry: Although individual lots with quality as bad as the acceptance quality
28、limit may be accepted with fairly high probability, the designation of an acceptance quality limit does not suggest that this is a desirable quality level. Sampling schemes found in International Standards such as this part of ISO 2859, with their rules for switching and for discontinuation of sampl
29、ing inspection, are designed to encourage suppliers to have process averages consistently better than the AQL. Otherwise, there is a high risk that the inspection severity will be switched to tightened inspection under which the criteria for lot acceptance become more demanding. Once on tightened in
30、spection, unless action is taken to improve the process, it is very likely that the rule requiring discontinuation of sampling inspection pending such improvement will be invoked. SOURCE: ISO 2859-1:1999, definition 3.1.26 3.9 thickness variation difference between the largest and smallest of thickn
31、ess measurements, within a cover glass 3.10 vision 1,0 standard visual acuity ability to see an object so small that the angle subtended at the eye is only one minute of arc ( 1/60 of a degree) Note 1 to entry: At 0,6 m the size of a test object is about 1,75 mm. Note 2 to entry: Since slight colour
32、 variation is permitted, definition of colour vision quality of the observer is not critical. 4 Requirements 4.1 Transparency and colour The cover glass shall be transparent and colourless when observed as specified in 6.6. 4.2 Non-flatness (waviness) and non-parallelism 4.2.1 Non-flatness (waviness
33、) The cover glass shall be sufficiently free of waviness to pass the test as specified in 6.8.1.2 ISO 2013 All rights reservedBS ISO 8255-2:2013ISO 8255-2:2013(E) 4.2.2 Non-parallelism Thickness variation within a single cover glass with maximum length dimension of 60 mm shall be no more than half t
34、he total thickness tolerance in ISO 8255-1, when tested according to 6.8.2. Of the sample of 100 cover glasses, accept a maximum of five that do not meet this requirement. 4.3 Durability Glass shall have a surface of sufficient chemical durability and resistance to atmospheric attack to pass the sol
35、ubility test specified in 6.9. 4.4 Surface quality and inclusions Glass shall be visibly free of pits, seeds, cords, stones, lines, abrasions, scratches, or cracks when observed as specified in 6.5 (AQL 1,5). 4.5 Edge finish The cover glass shall have no chipped corners or nicked edges exceeding 1 m
36、m in length and 0,5 mm in depth (AQL 1,0) when examined as specified in 6.7. 4.6 Cleanliness and cloudiness Cover glasses shall be clean and free of cloudiness, fingerprints, or particulate matter on its surface when observed as specified in 6.4 (AQL 1,5). 4.7 Adhesion Cover glass shall be free from
37、 adhesion when tested as specified in 6.3 (AQL 1,5). 4.8 Residual stress/birefringence Cover glass designated for use with polarized light shall not exhibit an optical path difference greater than 5 nm when measured through principal plane of the cover glass as specified in 6.10 (Inspection level S-
38、2. AQL 1,0). 5 Sampling 5.1 General The information in 5.2 may be sufficient to ensure compliance if the manufacturers certificate of conformance with ISO 9001 has been accepted by the purchaser or user. If product is to be marked “conforms with ISO 8255-2”, testing shall be as stated in Clause 6 wi
39、th samples drawn as specified in 5.3. 5.2 Quality measurement for cover glasses for conformance with this part of ISO 8255 The sampling methods, inspection levels, and AQLs in this part of ISO 8255 are required for finished lot inspection. If a producer has a “Quality system”, as specified in ISO 90
40、01, and this system meets the quality expectations of the purchaser or user, the suppliers certificate of conformance may be acceptable to the purchaser or user. Manufacturers carry out in-process inspection to ensure compliance. Cloudiness and cleanliness might be worth evaluating with inspection l
41、evels and AQL on a lot-by-lot basis. In-process, inspection may be used by the manufacturer to ensure compliance with other criteria to qualify lots for certification. ISO 2013 All rights reserved 3BS ISO 8255-2:2013ISO 8255-2:2013(E) Even if the suppliers certificate of conformance is acceptable to
42、 the purchaser or user, such lots shall not be marked, “Conforms with ISO 8255-2”, unless tested as specified in Clause 6 with samples drawn as specified in 5.3. 5.3 Drawing of samples and units inspection Samples shall be drawn at random from a lot of cover glass according to procedures outlined in
43、 ISO 2859-1, Normal Inspection, General Inspection level I, or, when specified, inspection level S-1, S-2, S-3, or S-4, with samples sizes chosen according to Annex A, Tables A .1 and A.2, unless a specific number of samples randomly selected from the total sample population is specified. The unit o
44、f inspection shall be one cover glass, except for packaging requirements, in which case the unit of inspection shall be one package. The samples shall be handled in a way which does not affect their cleanliness, or cause them to stick together, preferably by use of rubber or plastic finger covers. M
45、ultiple defects on a single piece shall be considered one defective piece. When fewer than the total sample are required for a test, the population for an individual test shall be randomly selected from within the sample chosen by the method specified in paragraph one of this subclause. Samples may
46、be reused in subsequent tests. A cover glass with multiple defects within a single attribute shall be considered a single defective item. 6 Test methods 6.1 General All observations shall be made by the unaided eye corrected to vision 1,0 (without magnification). Illumination is to be diffuse unifor
47、m artificial light produced with a “cool white” fluorescent lamp, or equivalent, with intensity of (1 500 150) lx. All testing, to be valid, must be performed within six months from the date of packaging. Testing shall be carried out in the following order: a) packaging: suitability and labelling; b
48、) adhesion; c) cleanliness; d) freedom from pits, etc.; e) transparency and colour; f) edge finish; g) non-flatness (waviness) and non-parallelism; h) durability (chemical durability of surface and resistance to atmospheric attack; solubility); i) residual stress/birefringence. 6.2 Package suitabili
49、ty The individual packages, selected as specified in Clause 5, shall be examined to determine that the package is designed so that the cover glass may be removed easily by the gripping edges without contaminating surfaces and without causing lint or plastic foam particles to fall onto the glass surface, and that it may be easily reclosed without damage to the contents or risk of spillage.4 ISO 2013 All rights reservedBS ISO 8255-2:2013ISO 8255-2:2013(E) The average count or mass shall be at least as much as stated on the l
