1、raising standards worldwide NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BSI Standards Publication BS ISO 8578:2012 Microscopes Marking of objectives and eyepiecesBS ISO 8578:2012 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 8578:
2、2012. It supersedes BS 7012-15:1997 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CPW/172/5, Optics and Photonics - Microscopes. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does
3、 not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2012. Published by BSI Standards Limited 2012 ISBN 978 0 580 71631 7 ICS 37.020 Compliance with a British Standard cannot confer immunity from lega
4、l obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2012. Amendments issued since publication Date T e x t a f f e c t e dBS ISO 8578:2012 ISO 2012 Microscopes Marking of objectives and eyepieces Microscopes Marquage des ob
5、jectifs et des oculaires INTERNATIONAL STANDARD ISO 8578 Second edition 2012-06-01 Reference number ISO 8578:2012(E)BS ISO 8578:2012ISO 8578:2012(E) ii ISO 2012 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may
6、be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. +
7、 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in SwitzerlandBS ISO 8578:2012ISO 8578:2012(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing
8、International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaiso
9、n with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of tec
10、hnical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is dra
11、wn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 8578 was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and end
12、oscopes. This second edition cancels and replaces the first edition (ISO 8578:1997) which has been technically revised. It also incorporates the Technical Corrigendum ISO 8578:1997/Cor. 1:2002. ISO 2012 All rights reserved iiiBS ISO 8578:2012ISO 8578:2012(E) Introduction This International Standard
13、has been prepared in order to define clearly the data relating to optical characteristics with which microscope objectives and eyepieces have to be marked and the positioning of such marking on the component to enable correct use of the microscope. In addition to data which have been marked, recomme
14、ndations for the marking of additional information relating to several other optical characteristics are given. iv ISO 2012 All rights reservedBS ISO 8578:2012Microscopes Marking of objectives and eyepieces 1 Scope This International Standard specifies the format for the marking of data or symbols f
15、or optical characteristics on microscope objectives and eyepieces and the positioning of the data. It makes recommendations for the marking of additional information, particularly colour coding of rings designating the magnification of objectives and the immersion media with which they are used. 2 N
16、ormative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 8036, Optics and photonic
17、s Microscopes Immersion liquids for light microscopy ISO 19012-1, Microscopes Designation of microscope objectives Part 1: Flatness of field/Plan ISO 19012-2, Optics and photonics Designation of microscope objectives Part 2: Chromatic correction 3 Objectives 3.1 Mandatory markings on objectives The
18、markings on objectives shall be as given in Table 1. Tube length and cover glass thickness shall be marked in this sequence and shall be separated by oblique strokes and be in one line, e.g. /0.17, 160/-, /0. This marking shall be in a smaller font than markings according to Table 3. The objective f
19、ield number (OFN) shall be marked according to the following example: OFN25 3.2 Recommended additional markings on objectives The marking of additional data is optional. If features as listed in Table 2 are marked, they shall be marked as given in Table 2. 3.3 Recommendation for arrangement of the m
20、arking It is recommended that the markings in Table 3, column A should be placed above or before the markings of column B, which in turn should be placed above or before those of column C. 4 Eyepieces 4.1 Mandatory markings on eyepieces The markings on eyepieces shall be as specified in Table 4. 4.2
21、 Recommended additional markings on eyepieces The marking of additional data is optional. If features as listed in Table 5 are marked, they shall be marked as given in Table 5. INTERNATIONAL STANDARD ISO 8578:2012(E) ISO 2012 All rights reserved 1BS ISO 8578:2012ISO 8578:2012(E) Table 1 Mandatory ma
22、rkings on objectives Optical property Feature to be marked Example of marking a Remarks Magnification Lateral magnification of objectives for a finite image distance. 100 Magnification and numerical aperture shall be separated by an oblique stroke, e.g. 100/1.30. Lateral magnification of objectives
23、for an infinite image distance. 100 The marked value of the magnification of infinity corrected objectives is only valid in combination with the related tube lens. The marking of the symbol “” has been introduced as an additional designation of magnification on infinity-corrected objectives. Apertur
24、e Numerical aperture. /1.30 The numerical aperture shall be stated to at least two decimal places. Adjustable iris diaphragm Limiting values of numerical aperture. /1.30-0.80 The upper and lower limits of the numerical aperture range controlled by the iris diaphragm shall be marked at the position w
25、here the value for the numerical aperture is usually marked. Immersion liquid Oil according to ISO 8036. OIL For additional marking, colour-coded rings can be used (see Table 2). Water. W Glycerol according to ISO 8036. GLYC Other. The requirement to use any other immersion medium shall be indicated
26、 unambiguously. Tube length Length in mm for objective of finite primary image distance. 160 See 3.1. Symbol for objective of primary image distance . Cover glass thickness Thickness, in mm /0 For objectives that are corrected for use with uncovered specimens only, the figure “0” shall be indicated
27、after the first oblique stroke. /0.17 For objectives that are corrected for use only with a specified cover glass, the value of the cover glass thickness to be used shall be indicated, in mm, after the first oblique stroke, e.g. /0.17. /- For objectives that can be used without a cover glass or with
28、 a cover glass up to 0,17 mm thickness, the symbol “-” shall be positioned after the first oblique stroke. /0.14-0.20 For objectives with a correction collar, the range of usable cover glass thicknesses shall be given. Objective field number Diameter of intermediate image, in mm, according to ISO 19
29、012-1. /OFN20 Flatness of field Field flatness properties according to ISO 19012-1. PLAN Chromatic correction Chromatic correction properties according to ISO 19012-2. FL Example for semi-apochromat. APO Example for apochromat. Phase contrast Symbol PH. PH 2 A figure after the symbol indicates the a
30、ssociated annular diaphragm. aCapital or lower case letters optional. 2 ISO 2012 All rights reservedBS ISO 8578:2012ISO 8578:2012(E) Optical property Feature to be marked Example of marking a Remarks Polarizing microscopy Symbol POL, P or PO. POL PO PManufacturer Name or symbol of identification.aCa
31、pital or lower case letters optional. Table 2 Recommended additional markings on objectives Optical property Feature to be marked Example of marking a Remarks Magnification Value Colour of ring 1/1.25 Black 1.6/2 Grey 2.5/3.2 Brown 4/5 Red 6.3/8 Orange 10/12.5 Yellow 16/20 Light green 25/32 Dark gre
32、en 40/50 Light blue 63/80 Dark blue 100 125 160 White Immersion medium Medium Colour of ring To avoid confusion, it is recommended that a coloured ring indicating the immersion medium only be used in conjunction with a second ring indicating the magnification. Air None Oil Black Water White Glycerol
33、 Orange Others Red Phase contrast The entire marking, apart from the coloured rings and the manufacturers name, shall be in green.The marking of the manufacturers name may be in any colour. Polarizing microscopy The entire marking, apart from the coloured rings and the manufacturers name, shall be i
34、n red.The marking of the manufacturers name may be in any colour. Differential interference contrast Symbol DIC. DIC Epi illumination Symbol EPI or M. EPI MaCapital or lower case letters optional. Table 1 (continued) ISO 2012 All rights reserved 3BS ISO 8578:2012ISO 8578:2012(E) Optical property Fea
35、ture to be marked Example of marking a Remarks Epi illumination, brightfield and darkfield Symbol D or BD or HD. D BD HD The symbol EPI can be marked in addition. Correction collar Symbol CORR or KORR. CORR Long working distance Symbol L, LD or LWD. L LD LWDCountry of manufactureThe marking of the c
36、ountry of origin is mandatory in several countries. aCapital or lower case letters optional. Table 3 Recommendation of arrangement of markings on objectives A B C Flatness of field Chromatic correction Long working distance Magnification Numerical aperture Immersion liquid Phase contrast Polarizing
37、microscopy system Differential interference contrast Objectives for brightfield and darkfield Epi illumination Objectives with correction collar NOTE 1 If an additional coloured ring is used in accordance with Table 2 to identify the immersion medium, this ring should be placed closer to the front l
38、ens than the coloured ring used to indicate the magnification. NOTE 2 A to precede B, B to precede C (see 3.3). Table 4 Mandatory markings on eyepieces Optical property Feature to be marked Example of marking Remarks Magnification Visual magnification 10 Visual magnification and field-of-view number
39、 shall be separated by an oblique stroke, e.g. 10/18. Field-of-view Diameter in mm /18 Manufacturer Name or symbol of identificationTable 5 Recommended additional markings on eyepieces Optical property Feature to be marked Example of marking Remarks Suitable for spectacle wearers Symbol Marking in c
40、onjunction with the magnification and field-of-view number, for example 10/18 . Centred and aligned cross lines Symbol Only for eyepieces with centred cross lines aligned with an orienting pin. Table 2 (continued) 4 ISO 2012 All rights reservedBS ISO 8578:2012BS ISO 8578:2012 ISO 8578:2012(E) ISO 20
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