1、BRITISH STANDARD BS ISO 8900:2005 Oil of bergamot petitgrain Citrus bergamia (Risso et Poit.) ICS 71.100.60 BS ISO 8900:2005 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 14 November 2005 BSI 14 November 2005 ISBN 0 580 46829 1 National for
2、eword This British Standard reproduces verbatim ISO 8900:2005 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can
3、be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of th
4、e BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enq
5、uirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This docume
6、nt comprises a front cover, an inside front cover, the ISO title page, pages ii to iv, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments Refe
7、rence number ISO 8900:2005(E)INTERNATIONAL STANDARD ISO 8900 Second edition 2005-10-15 Oil of bergamot petitgrain Citrus bergamia (Risso et Poit.) Huile essentielle de petitgrain bergamotier Citrus bergamia (Risso et Poit.) BS ISO 8900:2005ii iii Contents Page Foreword iv 1 Scope . 1 2 Normative ref
8、erences . 1 3 Terms and definitions. 1 4 Requirements 1 4.1 Appearance . 1 4.2 Colour 1 4.3 Odour . 1 4.4 Relative density at 20 C, 20 20 d 1 4.5 Refractive index at 20 C 1 4.6 Optical rotation at 20 C . 2 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C 2 4.8 Acid value 2 4.9 Ester value
9、. 2 4.10 Chromatographic profile 2 4.11 Flashpoint 2 5 Sampling 2 6 Test methods. 2 6.1 Relative density at 20 C, 20 20 d 2 6.2 Refractive index at 20 C 2 6.3 Optical rotation at 20 C . 2 6.4 Miscibility in ethanol 70 %, (volume fraction), at 20 C. 2 6.5 Acid value 2 6.6 Ester value. 2 6.7 Chromatog
10、raphic profile 2 7 Packaging, labelling, marking and storage 2 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of bergamot petitgrain Citrus bergamia (Risso et Poit.). 3 Annex B (informative) Flashpoint .5 Bibliography . 6 BS ISO 8900:2005iv For
11、eword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a tech
12、nical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
13、 electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated t
14、o the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for
15、 identifying any or all such patent rights. ISO 8900 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 8900:1987), which has been technically revised. BS ISO 8900:20051 Oil of bergamot petitgrain Citrus bergamia (Risso et P
16、oit.) 1 Scope This International Standard specifies certain characteristics of the oil of bergamot petitgrain Citrus bergamia (Risso et Poit.), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this do
17、cument. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labell
18、ing and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 709, Essential oils Determination
19、of ester value ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essenti
20、al oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of bergamot petigrain essential oil obtained by steam distil
21、lation of the leaves and/or the small fruits left in the twigs of Citrus bergamia (Risso et Poit.), of the Rutaceae family NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear liquid. 4.2 Colour Pale yellow or amber. 4.3 Odour Characteristic. 4.4 Relative den
22、sity at 20 C, 20 20 d Minimum: 0,886 Maximum: 0,894 4.5 Refractive index at 20 C Minimum: 1,460 0 Maximum: 1,465 0 BS ISO 8900:20052 4.6 Optical rotation at 20 C Between 4 and +3 . 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes of eth
23、anol 70 % (volume fraction) to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 2 4.9 Ester value Minimum: 128 Maximum: 195 4.10 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the represen
24、tative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato- graphic profile of the essential oil. Table 1 Chromatographic profile Components Minimum % Maximum
25、 % -Pinene 1,2 1,8 Myrcene 1,2 1,8 Limonenea2,3 3,0 trans- -Ocimene 1,2 1,6 Linalol 9,0 12,3 Linalyl acetate 63,8 71 -Caryophyllene 1,0 1,2 -Terpineol 2,0 2,7 Neryl acetate 2,0 2,4 Geranyl acetate 2,2 2,7 Nerol 0,4 0,8 a The limonene is regarded to be predominantly L-limonene based on wet physical t
26、esting. It is believed that there might be a small amount of D-limonene present but the exact quantity is unknown. NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.11 Flashpoint Information on the flashpoint is given in Annex B. 5 S
27、ampling See ISO 212. Minimum volume of test sample: 50 ml NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotati
28、on at 20 C See ISO 592. 6.4 Miscibility in ethanol 70 %, (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Ester value See ISO 709. 6.7 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 89
29、00:20053 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of bergamot petitgrain Citrus bergamia (Risso et Poit.) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 -Pinene Myrcene Limonene trans- -Ocimene Linalol -Terpineol Nero
30、l Geraniol Linalyl acetate Neryl acetate Geranyl acetate -Caryophyllene Column: silica capillary; length 30 m; internal diameter 0,20 mm Stationary phase: 5% diphenylpolysiloxane 95% dimethylpolysiloxane (SP-5 ) Film thickness: 0,20 m Oven temperature: temperature programming from 60 C to 180 C at a
31、 rate of 1,75 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 8900:20054 Peak identification Op
32、erating conditions 1 2 3 4 5 6 7 8 9 10 11 12 -Pinene Myrcene Limonene trans- -Ocimene Linalol Linalyl acetate -Caryophyllene -Terpineol Neryl acetate Geranyl acetate Nerol Geraniol Column: silica capillary; length 30 m; internal diameter 0,20 mm Stationary phase: polyethylene glycol (Supercowax-10
33、) Film thickness: 0,20 m Oven temperature: temperature programming from 60 C to 180 C at a rate of 1,75 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A
34、.2 Typical chromatogram taken on a polar column BS ISO 8900:20055 Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cas
35、es are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that there is wide variation in the chemical composition of essential oils; the volume of the samp
36、le needed in certain requirements would be too costly for high- priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpo
37、int in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information see ISO/TR 11018. B.2 Flashpoint of the essential oil of bergamot petitgrain The mean
38、 value is +75 C. NOTE Obtained with “Pensky Martens“ equipment. BS ISO 8900:20056 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 8900:2005blankBS ISO 8900:2005 BSI 389 Chiswick High Road Lond
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