1、BSI Standards Publication BS ISO 9022-11:2015 Optics and photonics Environmental test methods Part 11: Mould growthBS ISO 9022-11:2015 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 9022-11:2015. It supersedes BS ISO 9022-11:1994 which is withdrawn. The UK p
2、articipation in its preparation was entrusted to Technical Committee CPW/172, Optics and Photonics. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are re
3、sponsible for its correct application. The British Standards Institution 2015. Published by BSI Standards Limited 2015 ISBN 978 0 580 88209 8 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the St
4、andards Policy and Strategy Committee on 30 April 2015. Amendments/corrigenda issued since publication Date T e x t a f f e c t e dBS ISO 9022-11:2015 ISO 2015 Optics and photonics Environmental test methods Part 11: Mould growth Optique et photonique Mthodes dessais denvironnement Partie 11: Moisis
5、sures INTERNATIONAL STANDARD ISO 9022-11 Second edition 2015-04-15 Reference number ISO 9022-11:2015(E)BS ISO 9022-11:2015ISO 9022-11:2015(E)ii ISO 2015 All rights reserved COPYRIGHT PROTECTED DOCUMENT ISO 2015 All rights reserved. Unless otherwise specified, no part of this publication may be repro
6、duced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester
7、. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in SwitzerlandBS ISO 9022-11:2015ISO 9022-11:2015(E)Foreword iv Introduction vi 1 Scope . 1 2 Normative references 1 3 General information and test
8、conditions 1 3.1 Test fungi 1 3.2 Fungal spore suspension 2 3.2.1 Fungal cultures 2 3.2.2 Spore suspensions 2 3.2.3 Mixed spore suspension 3 3.3 Control strips 3 3.4 Specimen 4 3.5 Incubation cabinets and climatic chambers 5 4 Conditioning method 85: Mould growth 5 5 Procedure. 5 5.1 General . 5 5.2
9、 Preconditioning 6 5.3 Activities during conditioning . 6 5.4 Recovery . 6 5.5 Evaluation 6 5.6 General level for acceptance 6 6 Environmental test code . 7 7 Specification 7 Annex A (informative) Explanatory notes . 8 ISO 2015 All rights reserved iii Contents PageBS ISO 9022-11:2015ISO 9022-11:2015
10、(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which
11、 a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all mat
12、ters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This d
13、ocument was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or al
14、l such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents). Any trade name used in this document is information given for the convenience of users an
15、d does not constitute an endorsement. For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary i
16、nformation The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 1, Fundamental standards. This second edition cancels and replaces the first edition (ISO 9022-11:1994), of which it constitutes a minor revision. ISO 9022 consists of the following parts, und
17、er the general title Optics and photonics Environmental test methods: Part 1: Definitions, extent of testing Part 2: Cold, heat and humidity Part 3: Mechanical stress Part 4: Salt mist Part 6: Dust Part 7: Resistance to drip or rain Part 8: High internal pressure, low internal pressure, immersion Pa
18、rt 9: Solar radiation and weathering Part 11: Mould growth Part 12: Contamination Part 14: Dew, hoarfrost, ice Part 17: Combined contamination, solar radiation Part 20: Humid atmosphere containing sulfur dioxide or hydrogen sulfideiv ISO 2015 All rights reservedBS ISO 9022-11:2015ISO 9022-11:2015(E)
19、 Part 22: Combined cold, dry heat or temperature change with bump or random vibration Part 23: Low pressure combined with cold, ambient temperature and dry and damp heat ISO 2015 All rights reserved vBS ISO 9022-11:2015ISO 9022-11:2015(E) Introduction Optical instruments are affected during their us
20、e by a number of different environmental parameters which they are required to resist without significant reduction in performance and to remain within defined specifications. The type and severity of these parameters depend on the conditions of use of the instrument (for example, in the laboratory
21、or workshop) and on its geographical location. The environmental effects on optical instrument performance in the tropics and subtropics are totally different from those found when they are used in arctic regions. Individual parameters cause a variety of different and overlapping effects on instrume
22、nt performance. The manufacturer attempts to ensure, and the user naturally expects, that instruments will resist the likely rigours of their environment throughout their life. This expectation can be assessed by exposure of the instrument to a range of simulated environmental parameters under contr
23、olled laboratory conditions. The severity of these conditions is often increased to obtain meaningful results in a relatively short period of time. In order to allow assessment and comparison of the response of optical instruments to appropriate environmental conditions, ISO 9022 contains details of
24、 a number of laboratory tests which reliably simulate a variety of different environments. The tests are based largely on IEC standards, modified where necessary to take into account features special to optical instruments. As a result of continuous progress in all fields, optical instruments are no
25、 longer only precision- engineered optical products, but, depending on their range of application, also contain additional assemblies from other fields. For this reason, the principal function of the instrument is to be assessed to determine which International Standard should be used for testing. I
26、f the optical function is of primary importance, then ISO 9022 is applicable, but if other functions take precedence then the appropriate International Standard in the field concerned should be applied. Cases can arise where application of both ISO 9022 and other appropriate International Standards
27、will be necessary.vi ISO 2015 All rights reservedBS ISO 9022-11:2015Optics and photonics Environmental test methods Part 11: Mould growth WARNING Although the species of fungi selected for testing do not normally present a hazard to humans, certain people can develop allergies or other reactions. Th
28、e use of experienced and trained personnel is required to ensure the proper handling of fungi and the appropriate conduct of the tests. It is, therefore, recommended to entrust the performance of the tests required by this part of ISO 9022 to a microbiological laboratory, since such laboratories hav
29、e the appropriate equipment and trained personnel. 1 Scope This part of ISO 9022 specifies the methods relating to the environmental tests of optical instruments, including additional assemblies from other fields (e.g. mechanical, chemical, and electronic devices) under equivalent conditions, for th
30、eir ability to resist the influence of mould growth. However, complete instruments or assemblies are only tested as specified in this part of ISO 9022 in exceptional cases. Normally, representative specimens such as mounted optics, material samples, or surface coatings on representative samples are
31、used for testing. The tests described in this part of ISO 9022 are designed for the selection of materials and components for instruments likely to be used in an environment that is conducive to mould growth, rather than for regular production control. The purpose of testing is to investigate to wha
32、t extent the optical, climatic, mechanical, chemical and electrical (including electrostatic) performance characteristics of the specimen are affected by mould growth. In addition, the tests in this part of ISO 9022 are designed to assess to what extent metabolic waste products (such as enzymes or a
33、cids) excreted by fungi, cause etching, corrosion, or short-circuits on, for instance, printed circuit boards. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the ed
34、ition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 9022-1, Optics and photonics Environmental test methods Part 1: Definitions, extent of testing 3 General information and test conditions 3.1 Test fungi The species of fu
35、ngi selected for testing (see Table 1) are frequently found on optical glass surfaces. Among them are species of hydrophyl, mesophyl, and xerophyl fungi. INTERNATIONAL ST ANDARD ISO 9022-11:2015(E) ISO 2015 All rights reserved 1BS ISO 9022-11:2015ISO 9022-11:2015(E) Table 1 Test fungi Series No. Spe
36、cies 1 2 3 4 5 6 7 8 9 10 Aspergillus niger Aspergillus flavus Aspergillus versicolor Trichoderma viride Penicillium funiculosum Penicillium citrinum Paecilomyces Chaetomium globosum Eurotium tonophilum Aspergillus penicilloiden (Vitrocolae) Since strains of fungi change their characteristics with t
37、ime, only fungal species are specified. The test report or relevant specification, respectively, shall, however, specify the fungal strains used for testing. 3.2 Fungal spore suspension 3.2.1 Fungal cultures Pure cultures of each of the fungus species specified in Table 1 shall be maintained separat
38、ely on an appropriate agar medium (e.g. malt agar). The fungal cultures used for the spore suspension shall not be older than 14 days to 21 days and shall not be used more than once for preparing a mixed spore suspension. 3.2.2 Spore suspensions For preparing the spore suspensions, and wherever else
39、 in this Subclause “water” is specified, use distilled or fully demineralized sterile water containing 0,05 % (mass fraction) of a non-toxic wetting agent such as sodium dioctylsulfosuccinate or sodium laurylsulfate. Pour 10 ml of the water into each of the fungal cultures described in 3.2.1. Using
40、a sterile platinum loop or any other suitable means, carefully scrape the spores from the mycelial mat. Take care to leave out clumps of agar. Pour the spores charge into a sterile Erlenmeyer flask containing 45 ml of water. Add sterile solid glass beads and shake vigorously to liberate the spores f
41、rom the fruiting body and to break the spore clumps. Filter the dispersed fungal spore suspension through sterile glass wool to remove mycelial fragments. Centrifuge the filtrate and discard the supernatant liquid. Resuspend the residue in 50 ml of water and centrifuge. Wash the spores obtained from
42、 each of the fungi in this manner three times. Dilute the final washed residue with the mineral salts solution specified in Table 2 in such a manner that each resultant spore suspension contains (1 000 000 200 000) spores per millilitre, measured using a suitable counting chamber.2 ISO 2015 All righ
43、ts reservedBS ISO 9022-11:2015ISO 9022-11:2015(E) Table 2 Mineral salts solution Component Mass g Potassium dihydrogen orthophosphate (KH 2 PO 4 ) 0,7 Potassium monohydrogen orthophosphate (K 2 HPO 4 ) 0,7 Magnesium sulfate heptahydrate (MgSO 4 .7H 2 O) 0,7 Ammonium nitrate (NH 4 NO 3 ) 1,0 Sodium c
44、hloride (NaCl) 0,005 Iron(II) sulfate heptahydrate (FeSO 4 .7H 2 O) 0,002 Zinc sulfate heptahydrate (ZnSO 4 .7H 2 O) 0,002 Manganese(II) sulfate monohydrate (MnSO 4 .H 2 O) 0,001 Distilled water (H 2 O) 1 000,0 Sterilize the mineral salts solution in an autoclave at 120 C for 20 min. Using sodium hy
45、droxide solution, c(NaOH) = 0,01 mol/l, adjust the pH of the solution to 6,0 to 6,5 after sterilization. (Percentage purity of the chemicals: atomic adsorption spectroscopy.) Inoculate each of 10 Petri dishes containing an appropriate agar medium (e.g. malt agar) with spore suspension and immediatel
46、y incubate the dishes, to check the viability of each fungus species, in the incubation chamber to be used for exposing the specimens. In the event that fungicide-treated specimens are under test in the incubation chamber, expose the Petri dishes to exactly the same climatic conditions in a separate
47、 incubation chamber. The absence of growth of any of the various fungus species, at the end of one week, will invalidate the results of all simultaneously performed tests using these spores. Such invalidated tests shall be repeated using freshly prepared mixed spore suspensions from new cultures. 3.
48、2.3 Mixed spore suspension After having taken the inoculum from the spore suspensions for the purpose described in 3.2.2, blend equal portions of the 10 spore suspensions to obtain the final mixed spore suspension. The spore suspensions from the individual cultures as well as the mixed spore suspens
49、ion shall be used on the day of their preparation. On no account shall they be stored for later use. 3.3 Control strips Along with the specimens place at least three control strips in the exposure chamber in order to ensure that optimal climatic conditions are present in the incubation or climatic chamber during exposure of the contaminated specimens. The control strips are of no use if the specimens have been previously treated with fungicides; since these become active predominantly during the
