1、Designation: D4135 82 (Reapproved 2012)Standard Practice forSampling Phytoplankton With Depth-Integrating Samplers1This standard is issued under the fixed designation D4135; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year
2、of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This practice covers the procedures for obtaining quan-titative samples of a phytoplankton community by use ofdepth
3、-integrating sampler. The method is suitable for use inflowing waters.1.2 This standard does not purport to address all of thesafety problems, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the
4、 applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2D4137 Practice for Preserving Phytoplankton Samples3. Summary of Practice3.1 A depth-integrated sample is collected by means of asampler that integrates stream discharge as a function of depth.The veloc
5、ity of flow in a stream, as well the density ofphytoplankton, varies both vertically and horizontally. Depthintegration is used to collect a sample that is weightedaccording to the velocity at each increment of depth.3Thephytoplankton may be preserved as dictated by the objectivesof the study.4. Sig
6、nificance and Use4.1 The advantages of the depth-integrating samplers are asfollows:4.1.1 The samplers provide the only means by which arepresentative sample of phytoplankton can be obtained from astream.4.1.2 The variety of sampler designs result in the availabil-ity of a sampler that can be used i
7、n virtually all velocities offlow.4.1.3 Samples of known volumes can be obtained.4.1.4 A quantitative sample is obtained. Nanno- and ultra-plankton are not lost from the sampling device.4.1.5 Some of the samplers can be used in water up to 55 mdeep.4.1.6 Many of the samplers are light-weight and can
8、 be usedwithout auxiliary equipment.4.2 The disadvantages of the depth-integrating samplers areas follows:4.2.1 The samplers can be used only in flowing streamshaving a velocity greater than 1.5 ft3/s.4.2.2 Some of the samplers are heavy and require the use ofauxiliary equipment, such as a crane wit
9、h hoist.4.2.3 The collection of samples can be very time-consuming.4.3 There are several special considerations that shall beobserved when using depth-integrating samplers. They are asfollows:4.3.1 The nozzle of the sampler should be inspected peri-odically for chips, cracks, or other signs of damag
10、e andreplaced as necessary.4.3.2 The sample from each vertical profile must be com-bined with other similar samples in a common container. If thecombined sample is subsequently subdivided, it must bethoroughly mixed before doing so.5. Apparatus5.1 Several depth-integrating samplers are in widespread
11、use in water-quality studies and have been described bySubcommittee D19.07 on Sedimentation,4(Figs. 1-3). All thesamplers have the same basic design. They consist of ahydrodynamically designed, weighted body into which isplaced the sample container. The rear of the sampler containsseveral vanes for
12、stabilizing the sampler into the direction ofstream flow. The size and weight of the samplers vary. Thecommon sampler types have been designated as DH-49,DH-74, P-61, and D-77 by Subcommittee D19.07.Fig. 31This practice is under the jurisdiction of ASTM Committee E47 on BiologicalEffects and Environ
13、mental Fate and is the direct responsibility of SubcommitteeE47.01 on Aquatic Assessment and Toxicology.Current edition approved Sept. 1, 2012. Published Octber 2012. Originallyapproved in 1982. Last previous edition approved in 2004 as D4135 82 (2004).DOI: 10.1520/D4135-82R12.2For referenced ASTM s
14、tandards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Colby, B. R., “Relationship of Sediment Discharge to Streamflow,” U.S.Geology Surv
15、ey, Open-File Report, 1956, 170 pp.4Subcommittee on Sedimentation, Interagency Committee on Water Resources,“Determination of Fluvial Sediment Discharge,” Federal Interagency Commissionon Water Resources, Minneapolis, MN, Report, Vol 14, 1963, 151 pp.Copyright ASTM International, 100 Barr Harbor Dri
16、ve, PO Box C700, West Conshohocken, PA 19428-2959. United States16. Procedure6.1 One of the best sampling techniques currently acceptedby hydrologists for use in such situations is the equal-transitrate (ETR) method.5In this method, the standard suspended-sediment sampler is used to collect a discha
17、rge-weightedsample. Samples are taken at a number of equally spacedverticals in the cross section. The transit rate of the sampler,which is the rate of movement of the sampler from the watersurface to the stream bed and back to the surface, should be thesame at all verticals. Samples collected in ea
18、ch vertical arecomposited into a single sample that is representative of theentire flow in the cross section. Preserve the plankton asdescribed in Practice D4137.5Guy, H. P., and Norman, V. W., “Field Methods of Measurement of FluvialSediment,” U.S. Geological Survey, Technology of Water-Resources I
19、nvestigations,Book 3, Chapter C2, 1970, 59 pp.(a) D-11-48FIG. 1 Depth-Integrating SamplersD4135 82 (2012)2(b) D-77FIG. 2 Depth-Integrating SamplersD4135 82 (2012)3ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this s
20、tandard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be re
21、viewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of therespons
22、ible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West
23、Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).(c) P-61FIG. 3 Depth-Integrating SamplersD4135 82 (2012)4
