ASTM D6503-1999(2009) 402 Standard Test Method for Enterococci in Water Using Enterolert《用结肠镜对水中肠球菌的标准试验方法》.pdf
《ASTM D6503-1999(2009) 402 Standard Test Method for Enterococci in Water Using Enterolert《用结肠镜对水中肠球菌的标准试验方法》.pdf》由会员分享,可在线阅读,更多相关《ASTM D6503-1999(2009) 402 Standard Test Method for Enterococci in Water Using Enterolert《用结肠镜对水中肠球菌的标准试验方法》.pdf(6页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: D 6503 99 (Reapproved 2009)Standard Test Method forEnterococci in Water Using Enterolert1, 2This standard is issued under the fixed designation D 6503; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last r
2、evision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers a simple procedure for thedetection of enterococci in water and wastewater. It is based onIDEXXs pat
3、ented Defined Substrate Technology (DST).2Thisproduct, Enterolert, utilizes a nutrient indicator that fluoresceswhen metabolized. It can detect these bacteria at one colonyforming unit (CFU)/100 mL within 24 h. The presence of thismicroorganism in water is an indication of fecal contaminationand the
4、 possible presence of enteric pathogens.1.2 This test method can be used successfully with drinkingwater, source water, recreational (fresh and marine) water, andbottled water. It is the users responsibility to ensure thevalidity of this test method for waters of untested matrices.1.3 The values sta
5、ted in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety an
6、d health practices and determine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:3D 1129 Terminology Relating to WaterD 1193 Specification for Reagent WaterD 2777 Practice for Determination of Precision and Bias ofApplicable Test Methods of Committ
7、ee D19 on WaterD 3370 Practices for Sampling Water from Closed Conduits3. Terminology3.1 DefinitionsFor definitions of terms used in this testmethod, refer to Terminology D 1129.3.2 Definitions of Terms Specific to This Standard:3.2.1 enterococci, na gram positive bacteria possessingthe enzyme b-D-g
8、lucosidase, which cleaves the nutrient indi-cator and produces fluorescence under a long wave length (366nm) ultraviolet (UV) light.3.2.2 most probable number (MPN), na statistical methodfor determining bacterial density based on the Poisson distri-bution.3.2.3 presence-absence, na term used to indi
9、cate if en-terococci is present in a water sample. It is a qualitative value,“yes” or “no” for reporting results.3.2.4 quanti-tray2, na system for the quantification ofenterococci. It consists of a sealer and trays which havemulti-wells and can enumerate up to 2000 CFU/100 mLwithout dilution.3.2.5 s
10、nap pack, na package containing Enterolert reagentfor testing 100-mL sample either in the P/A format or quanti-tatively, that is, Quanti-Tray2system).4. Summary of Test Method34.1 This test method is used for the detection of enterococci,such as E. faecium, E. faecalis in drinking water, source wate
11、r,recreational waters (marine water and fresh), and bottled water.When the reagent is added to the sample and incubated at 41 60.5C for 24 h, Enterolert can detect these bacteria at 1CFU/100 mL. Fluorescence is produced when enterococcimetabolizes the nutrient indicator. Enterolert can be used as ap
12、resence-absence test or for quantification (5-tube, 10-tubeMPN, 15-tube serial dilution or the Quanti-Tray system).5. Significance and Use5.1 This test provides an easy and reliable method for thedetection of enterococci in water within 24 h. For recreationalwater (fresh and marine) testing is perfo
13、rmed to insure areasare safe for swimming. Enterolert also can be used for testingbottled water and drinking water.6. Interferences6.1 The presence of Bacillus spp. can interfere with thetesting of marine water samples. To eliminate interference, a1:10 dilution is required with sterile water (deioni
14、zed ordistilled).7. Apparatus7.1 Ultraviolet Lamp, 6-watt long wavelength (366 nm).7.2 41C Incubator (60.5C), air or water bath.7.3 Vessels, sterile, nonfluorescent.7.4 Quanti-Tray Sealer.21This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of S
15、ubcommittee D19.24 on Water Microbiology.Current edition approved May 1, 2009. Published June 2009. Originallyapproved in 1999. Last previous edition approved in 2005 as D 6503 99 (2005).2Trademark of IDEXX Laboratories, One Idexx Dr., Westbrook, ME 04092.3For referenced ASTM standards, visit the AS
16、TM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, Unit
17、ed States.7.5 Quanti-Tray or Quanti-Tray 2000.28. Reagents and Materials8.1 Purity of WaterUnless otherwise indicated, referencesto water shall be understood to mean reagent water conformingto Specification D 1193, Type IV. Sterilize the water by eitherautoclaving or by sterile filtration (0.22 micr
18、on-filtered water).8.2 Enterolert Test Kit.29. Precautions9.1 The analyst must observe the normal good laboratorypractices and safety procedures required in a microbiologylaboratory while preparing, using, and disposing of cultures,reagents and materials and while operating sterilization equip-ment
19、and other equipment.10. Sampling10.1 Collect the sample as described in detail in the USEPAmicrobiological methods manual4and in accordance withPractices D 3370.10.2 Sample Storage Temperature and HandlingConditionsIce or refrigerate water samples at a temperatureof 2 to 8C during transit to the lab
20、oratory. Use insulatedcontainers to ensure proper maintenance of storage tempera-tures. Take care that sample bottles are not totally immersed inwater during transit or storage.10.3 Holding Time LimitationsExamine samples, as soonas possible, after collection. Do not hold samples longer than6 h betw
21、een collection and initiation of analyses.11. Quality Control Check11.1 Check and record temperatures in incubators daily toensure temperature is within stated limits.11.2 Quality control should be conducted on each new lot ofEnterolert. See package insert for the recommended qualitycontrol procedur
22、e, which consists of the following protocol:11.2.1 For each type of the American Type Culture Collec-tion (ATCC) bacterial strain listed below, streak the cultureonto labeled TSA or blood agar plates and incubate at 35C for18 to 24 h.11.2.2 For each bacterial strain, touch a 1-l loop to acolony and
23、use it to inoculate a labeled test tube containing5 mL of sterile deionized water. Close cap and shake thor-oughly.11.2.3 For each bacterial strain, take a 1-l loop from thetest tube and use it to inoculate a labeled vessel containing100 mL.11.2.4 Follow the Enterolert presence/absence steps listeda
24、bove to test these controls. Compare the test results to thefollowing expected results:Control ATTC No. Expected ResultEnterococcus faecium 335667 FluorescenceSerratia marcescens (g, ) 43862 No fluorescenceAerococcus viridians (g, +) 10400 No fluorescence12. Procedure12.1 Presence/AbsenceSee package
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
本资源只提供5页预览,全部文档请下载后查看!喜欢就下载吧,查找使用更方便
5000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- ASTMD650319992009402STANDARDTESTMETHODFORENTEROCOCCIINWATERUSINGENTEROLERT 结肠 水中 球菌 标准 试验 方法 PDF

链接地址:http://www.mydoc123.com/p-522704.html