1、Designation: D7789 12Standard Practice forCollection of Fungal Material from Surfaces by Swab1This standard is issued under the fixed designation D7789; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A n
2、umber in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 The purpose of this standard practice is to describe theprocedures for collection of surface samples using sterileswabs.1.2 The purpose
3、of this standard practice is to support thefield investigator in differentiating fungal materials from non-fungal material such as scuffs, soot deposits, stains, pigments,dust, efflorescence, adhesives, dust, and water stains.1.3 This practice does not address building occupantexposures, or occupant
4、 health risks.1.4 The samples collected by this practice are appropriatefor culture, direct microscopy, and/or biochemical analysis.1.5 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establ
5、ish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD6044 Guide for Representative Sampling for Managementof Waste and Contamin
6、ated Media3. Terminology3.1 Definitions:3.1.1 sample, na portion of a population intended to berepresentative of the whole. a portion of a population.Aportionof material that is taken for testing or record purposes. D60443.1.2 sampling, na process consisting of the withdrawal orisolation of a fracti
7、onal part of the whole. D13563.2 Definitions of Terms Specific to This Standard:3.2.1 area (surface, sampled), na defined, measured ex-tent of surface sampled.3.2.2 biochemical analysis, nact of assessing chemicalsubstances (that is, chitin, enzyme activity, ergosterol,mycotoxin, DNA) originating fr
8、om living or dead organisms.3.2.3 chain of custody (COC), na document that providesfor the traceable transfer of field samples to the analyticallaboratory. It may or may not be combined with the Field DataSheet.3.2.4 culture analysis, nact of assessing microorganisms(if present) capable of growing o
9、n selected media underartificial and controlled conditions.3.2.5 direct microscopy analysis, nact of assessing micro-organisms (if present) using an optical compound microscope.3.2.6 field data sheet, na record of varying names thatprovides a reference document for information directly relatedto the
10、 sample collection event, including pre- and post-calibration data.3.2.7 fungi, neukaryotic, heterotrophic, absorptive organ-isms that develop a rather diffuse, branched, tubular body (thatis, network of hyphae) and reproduce by means of spores.3Theterms mold and mildew are frequently used by layper
11、sonswhen referring to various fungal colonization. For purposes ofthis standard, these terms have no technical meaning.3.2.8 fungal material, nfungal spores, hyphae and repro-ductive structures.4. Significance and Use4.1 This practice provides a procedure for collecting surfacematerial using a steri
12、le swab.4.2 A swab sample collected according to this practice isintended to be used to assess fungal material on surfaces.4.3 A swab sample collected from an area with defineddimensions can be used to quantify fungal material per unitarea or for qualitative analysis.4.4 Aswab sample collected from
13、point(s) of interest can beused for qualitative analysis or to quantify fungal material persample.1This practice is under the jurisdiction of ASTM Committee on Air Quality andis the direct responsibility of Subcommittee D22.08 on Sampling and Analysis ofMold.Current edition approved Aug. 15, 2012. P
14、ublished November 2012. DOI:10.1520/D778912.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Kendrick, Bryce,
15、 The Fifth Kingdom, 1992, 3rd Edition.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States14.5 A swab sample collected according to this standardpractice can be analyzed by direct microscopy, culture, orbiochemical analysis.5. Materials an
16、d Supplies5.1 Swabs5.1.1 Must be sterile and have a sterile transport container.5.1.2 May be used dry or wetted.5.1.2.1 Wetting/transport media (not required for dry swabs)must be appropriate for the intended analysis.5.2 Ruler, template or equivalent for measuring sample area.5.3 Permanent marking
17、pen or labels for sample identifica-tion.5.4 Chain of Custody (COC).5.5 Field Data Sheet, if separate from the COC.5.6 Temperature controlled transportation container, as ap-propriate.6. Procedure6.1 Document sample location/surface.6.2 Document the dimensions of the sampled area, ifappropriate.6.3
18、Select appropriate swab type (dry or wetted) to achieveoptimum collection efficiency.6.4 Remove swab from container and maintain sterility bytaking care not to contact any surface, except the area to besampled.6.4.1 For a dimensional area sample, rub swab acrosssampling area in a back and forth moti
19、on, both horizontally andvertically, slightly turning the swab with each pass until entirearea is sampled and the maximum material has been collectedfrom the surface.6.4.2 For a point of interest sample, touch the swab to thesurface.6.5 Return swab to sterile container for transport.6.6 Label the sa
20、mple container with a unique identifier.6.7 Complete Field Data Sheet in a physical or electronicformat.6.7.1 At a minimum, the sample field data sheet shallcontain the following information:6.7.1.1 Sampling date;6.7.1.2 Project name;6.7.1.3 Investigators name(s);6.7.1.4 Unique sample identifier;6.7
21、.1.5 Location of sample within structure; and6.7.1.6 Surface area dimensions if appropriate.6.8 Submit samples for analysis with Chain of Custody(COC), or Analysis Request Form, or both.6.8.1 At a minimum include:6.8.1.1 Name and signature of investigator;6.8.1.2 Date, time and signature of releasin
22、g party;6.8.1.3 Name and contact information of responsible party(that is, investigators employer);6.8.1.4 List unique sample identifier(s);6.8.1.5 Contact information for analytical report receipt, ifdifferent than submitters information;6.8.1.6 Specify analytical method(s);6.8.1.7 Turn-around time
23、(s) requested.NOTE 1Appendix X1 provides an example of the combined FieldData Sheet and Chain-of-Custody.6.8.2 Prepare samples for transport to the laboratory andsubmit to the laboratory as soon as practical following sam-pling.6.8.2.1 All samples should be packaged to protect fromdamage and tempera
24、ture extremes during transit.6.8.2.2 Package wet samples for culture or direct micro-scopic analysis with an ice pack (or similar) in an insulatedcontainer to stabilize the sample during shipping.6.8.2.3 Package samples for biochemical analysis as appro-priate for analysis.6.8.2.4 Ship samples via a
25、 traceable carrier, if not handdelivered.7. Interferences and Limitations7.1 The collection efficiency is a function of the texture ofthe surface sampled, the loading of the swab and whether theswab is dry or wetted.7.2 Not all types of fungal material are retained to the samedegree by the swab.7.3
26、This practice does not distinguish between the collectionefficiency of dry and wetted swabs.7.4 Swabs may lose material in the transport container.8. Keywords8.1 biochemical; culture; fungi; mold; mould; microscopy;particulate; surface sampling; swabD7789 122APPENDIX(Nonmandatory Information)X1. EXA
27、MPLE FIELD DATA SHEET/CHAIN-OF-CUSTODYX1.1 See Fig. X1.1.ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentioned inthis standard. Users of this standard are expressly advised that determination of the validity of any such paten
28、t rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited eithe
29、r for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair he
30、aring you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standar
31、d may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).D7789 123FIG. X1.1 Example Field Data Sheet/Chain-of-CustodyD7789124
