1、Designation: E 237 02 (Reapproved 2007)Standard Specification forLaboratory Glass Microvolumetric Vessels (VolumetricFlasks and Centrifuge Tubes)1This standard is issued under the fixed designation E 237; the number immediately following the designation indicates the year oforiginal adoption or, in
2、the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers volumetric flasks and fourtypes of centrifuge tubes, wide
3、ly used in microchemistry.NOTE 1Specifications for several items listed below were developedby the Committee on Microchemical Apparatus, Division of AnalyticalChemistry, American Chemical Society.21.2 Product with a stated capacity not listed in this standardmay be specified Class A tolerance when p
4、roduct conforms tothe tolerance range of the next smaller volumetric standardproduct listed in Table 1.2. Referenced Documents2.1 ASTM Standards:3E 438 Specification for Glasses in Laboratory ApparatusE 542 Practice for Calibration of Laboratory VolumetricApparatusE 671 Specification for Maximum Per
5、missible Thermal Re-sidual Stress in Annealed Glass Laboratory ApparatusE 694 Specification for Laboratory Glass Volumetric Appa-ratusE 920 Specification for Commercially Packaged LaboratoryApparatusE 921 Specification for Export Packaged Laboratory Appa-ratusE 1133 Practice for Performance Testing
6、of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE 1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3. Volumetric Flasks3.1 The volumetric flasks shall be of either Style I or StyleII of Fig. 1 and shall conform to the requirements of Fig. 1 andTa
7、ble 1. Each flask shall be marked with the symbol “TC” (or“IN”), the capacity, “20C”, and a 16 mm2marking area. (Allin an optional location on the flask.)4. Centrifuge Tubes4.1 Centrifuge tubes of four types shall conform to therequirements prescribed in Tables 2-4 and Figs. 2-5. The typesshown fit
8、into the metal shields supplied with commerciallyavailable centrifuges. Removal of the glass stoppers from thecentrifuge tubes shown in Fig. 3 and Fig. 4 is advisable beforecentrifugation. The four types of centrifuge tubes specified areas follows:4.1.1 Conical bottom, plain (Fig. 2).4.1.2 Conical b
9、ottom, stoppered (Fig. 3).4.1.3 Conical bottom, stoppered and graduated (Fig. 4).4.1.4 Cylindrical bottom, plain (Fig. 5).5. Sampling and Testing5.1 Refer to Specification E 1157.6. Calibration6.1 Flasks and graduated tubes shall be calibrated in accor-dance with Practice E 542 and conform to applic
10、able require-ments of Specification E 694. Glass shall be Type I, Class A ofSpecification E 438 and annealing shall be in accordance withSpecification E 671.7. Packaging7.1 Select from Specification E 920, Specification E 921,orPractice E 1133.8. Keywords8.1 glass, laboratory; microvolumetric; vesse
11、ls1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2007. Published December 2007. Originallyapproved in 1964. Last previous edition approved in 2002 as E 237
12、 02.2Committee on Microchemical Apparatus, Division of Analytical Chemistry,American Chemical Society, “Report on Recommended Specifications for Micro-chemical Apparatus. Volumetric Glassware: Flasks, Pipets, and Centrifuge Tubes,”Analytical Chemistry, Vol 28, 1956, p. 1993.3For referenced ASTM stan
13、dards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken,
14、PA 19428-2959, United States.TABLE 1 Volumetric Flask (see Fig. 1)Style Capacity, mLClass A/ Tolerance,6 mLA, ID, max/mm B, ID, ref/mm C, max/mm D, max/mm Stopper No.I 1 0.010 5.3 8.25 70 100 8II 1 0.010 5.3 95 125 8I 2 0.015 6.4 10.75 70 100 8I 3 0.015 6.4 13.50 72 100 8I 4 0.020 7.3 14.00 75 100 8
15、I 5 0.020 7.3 15.75 75 100 8I 10 0.020 8.25 18.00 110 135 9I 25 0.030 8.7 26.00 140 165 9NOTE 1The shape of the base may be either round or hexagonalFIG. 1 Volumetric Flasks (see Table 1)TABLE 2 Centrifuge Tubes, Conical Bottom, Plain (see Fig. 2)NominalCapacity,mLA,Height,mmB,ODCylindricalPortion,
16、mmC,ODTopFinish,mmD, Lengthof Taper, mmE,ODatBottom, mm0.5 586 26.06 0.25 13.0 6 1.0 30 6 23.56 0.51616 2 8.256 0.25 13.0 6 1.0 30 6 6 0.5266 2 10.75 6 0.25 13.5 6 1.0 30 6 2406 0.53746 2 10.75 6 0.25 13.5 6 1.0 30 6 .6 0.55 101 6 2 13.00 6 0.50 16.25 6 0.75 40 6 6 0.5TABLE 3 Centrifuge Tubes, Conic
17、al Bottom, Stoppered (see Fig. 3)NominalCapacity,mLA,Height,mmB,ODCylindricalPortion, mmC,ODTopFinish,mmD, Lengthof Taper,mmE,ODatBottom, mmF,Stopper No.0.5 66 6 26.06 0.25 13.0 6 1.0 30 6 23.56 0.5 detail G1696 2 8.25 6 0.25 13.0 6 1.0 30 6 6 0.5 detail H2806 2 10.75 6 0.25 13.5 6 1.0 30 6 2406 0.5
18、 93886 2 10.75 6 0.25 13.5 6 1.0 30 6 .6 0.5 951156 2 13.0 6 0.50 13.5 6 1.0 40 6 6 0.5 9E 237 02 (2007)2TABLE 4 Volumetric Tolerances of Centrifuge Tube,Conical Bottom, Stoppered and Graduated, mLAt 0.1 60.01At 0.2 60.02At 0.3 60.03At 0.4 60.05Above 0.4 60.075NOTE 1All dimensions are in millimetres
19、.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Interior of bottoms shall be rounded.FIG. 2 Centrifuge Tubes, Conical Bottom, Plain (see Table 2)NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Interior of bottoms shall
20、be rounded.FIG. 3 Centrifuge Tubes, Conical Bottom, Stoppered (see Table 3)E 237 02 (2007)3ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination
21、 of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdraw
22、n. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your co
23、mments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single o
24、r multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness shall be approximately 1 mm.N
25、OTE 3Interior of bottom shall be rounded.FIG. 4 Centrifuge Tube, Conical Bottom, Stoppered andGraduatedNOTE 1Capacity approximately 2.6 mL.NOTE 2All dimensions are in millimetres.NOTE 3Wall thickness shall be approximately 1 mm.NOTE 4Interior of bottom shall be rounded.FIG. 5 Centrifuge Tube, Cylindrical Bottom, PlainE 237 02 (2007)4
