1、Designation: E237 02 (Reapproved 2012)Standard Specification forLaboratory Glass Microvolumetric Vessels (VolumetricFlasks and Centrifuge Tubes)1This standard is issued under the fixed designation E237; the number immediately following the designation indicates the year oforiginal adoption or, in th
2、e case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers volumetric flasks and fourtypes of centrifuge tubes, widely
3、used in microchemistry.NOTE 1Specifications for several items listed below were developedby the Committee on Microchemical Apparatus, Division of AnalyticalChemistry, American Chemical Society.21.2 Product with a stated capacity not listed in this standardmay be specified Class A tolerance when prod
4、uct conforms tothe tolerance range of the next smaller volumetric standardproduct listed in Table 1.2. Referenced Documents2.1 ASTM Standards:3E438 Specification for Glasses in Laboratory ApparatusE542 Practice for Calibration of Laboratory VolumetricApparatusE671 Specification for Maximum Permissib
5、le Thermal Re-sidual Stress in Annealed Glass Laboratory ApparatusE694 Specification for Laboratory Glass Volumetric Appa-ratusE920 Specification for Commercially Packaged LaboratoryApparatusE921 Specification for Export Packaged Laboratory Appa-ratusE1133 Practice for Performance Testing of Package
6、d Labo-ratory Apparatus for United States Government Procure-mentsE1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3. Volumetric Flasks3.1 The volumetric flasks shall be of either Style I or StyleII of Fig. 1 and shall conform to the requirements of Fig. 1 andTable 1. Each
7、 flask shall be marked with the symbol “TC” (or“IN”), the capacity, “20C”, and a 16 mm2marking area. (Allin an optional location on the flask.)4. Centrifuge Tubes4.1 Centrifuge tubes of four types shall conform to therequirements prescribed in Tables 2-4 and Figs. 2-5. The typesshown fit into the me
8、tal shields supplied with commerciallyavailable centrifuges. Removal of the glass stoppers from thecentrifuge tubes shown in Fig. 3 and Fig. 4 is advisable beforecentrifugation. The four types of centrifuge tubes specified areas follows:4.1.1 Conical bottom, plain (Fig. 2).4.1.2 Conical bottom, stop
9、pered (Fig. 3).4.1.3 Conical bottom, stoppered and graduated (Fig. 4).4.1.4 Cylindrical bottom, plain (Fig. 5).5. Sampling and Testing5.1 Refer to Specification E1157.6. Calibration6.1 Flasks and graduated tubes shall be calibrated in accor-dance with Practice E542 and conform to applicable require-
10、ments of Specification E694. Glass shall be Type I, Class A ofSpecification E438 and annealing shall be in accordance withSpecification E671.7. Packaging7.1 Select from Specification E920, Specification E921,orPractice E1133.8. Keywords8.1 glass, laboratory; microvolumetric; vessels1This specificati
11、on is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2012. Published November 2012. Originallyapproved in 1964. Last previous edition approved in 2007 as E237 02(2007). DOI:10.15
12、20/E0237-02R12.2Committee on Microchemical Apparatus, Division of Analytical Chemistry,American Chemical Society, “Report on Recommended Specifications for Micro-chemical Apparatus. Volumetric Glassware: Flasks, Pipets, and Centrifuge Tubes,”Analytical Chemistry, Vol 28, 1956, p. 1993.3For reference
13、d ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Cons
14、hohocken, PA 19428-2959. United States1TABLE 1 Volumetric Flask (see Fig. 1)Style Capacity, mLClass A/ Tolerance,mLA, ID, max/mm B, ID, ref/mm C, max/mm D, max/mm Stopper No.I 1 0.010 5.3 8.25 70 100 8II 1 0.010 5.3 95 125 8I 2 0.015 6.4 10.75 70 100 8I 3 0.015 6.4 13.50 72 100 8I 4 0.020 7.3 14.00
15、75 100 8I 5 0.020 7.3 15.75 75 100 8I 10 0.020 8.25 18.00 110 135 9I 25 0.030 8.7 26.00 140 165 9NOTE 1The shape of the base may be either round or hexagonalFIG. 1 Volumetric Flasks (see Table 1)TABLE 2 Centrifuge Tubes, Conical Bottom, Plain (see Fig. 2)NominalCapacity,mLA,Height,mmB,ODCylindricalP
16、ortion, mmC,ODTopFinish,mmD, Lengthof Taper, mmE,ODatBottom, mm0.5 582 6.00.25 13.01.0 302 3.50.51 61 2 8.25 0.25 13.0 1.0 30 2 3.5 0.52 662 10.750.25 13.51.0 302 4.00.53 742 10.750.25 13.51.0 302 4.00.55 1012 13.000.50 16.250.75 402 4.00.5TABLE 3 Centrifuge Tubes, Conical Bottom, Stoppered (see Fig
17、. 3)NominalCapacity,mLA,Height,mmB,ODCylindricalPortion, mmC,ODTopFinish,mmD, Lengthof Taper,mmE,ODatBottom, mmF,Stopper No.0.5 66 2 6.0 0.25 13.0 1.0 30 2 3.5 0.5 detail G1 692 8.250.25 13.01.0 302 3.50.5 detailH2 802 10.750.25 13.51.0 302 4.00.5 93 882 10.750.25 13.51.0 302 4.00.5 95 1152 13.00.50
18、 13.51.0 402 4.00.5 9E237 02 (2012)2TABLE 4 Volumetric Tolerances of Centrifuge Tube,Conical Bottom, Stoppered and Graduated, mLAt 0.1 0.01At 0.2 0.02At 0.3 0.03At 0.4 0.05Above 0.4 0.075NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Inter
19、ior of bottoms shall be rounded.FIG. 2 Centrifuge Tubes, Conical Bottom, Plain (see Table 2)NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Interior of bottoms shall be rounded.FIG. 3 Centrifuge Tubes, Conical Bottom, Stoppered (see Table 3
20、)E237 02 (2012)3ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of
21、such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for ad
22、ditional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to
23、the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at t
24、he aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).NOTE 1All dimensions are in millimetres.NOTE 2Wall t
25、hickness shall be approximately 1 mm.NOTE 3Interior of bottom shall be rounded.FIG. 4 Centrifuge Tube, Conical Bottom, Stoppered and GraduatedNOTE 1Capacity approximately 2.6 mL.NOTE 2All dimensions are in millimetres.NOTE 3Wall thickness shall be approximately 1 mm.NOTE 4Interior of bottom shall be rounded.FIG. 5 Centrifuge Tube, Cylindrical Bottom, PlainE237 02 (2012)4
