ASTM E1482-2004 Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations《杀病毒剂功效评价中杀病毒剂中和作用的标准试验方法》.pdf
《ASTM E1482-2004 Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations《杀病毒剂功效评价中杀病毒剂中和作用的标准试验方法》.pdf》由会员分享,可在线阅读,更多相关《ASTM E1482-2004 Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations《杀病毒剂功效评价中杀病毒剂中和作用的标准试验方法》.pdf(3页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: E 1482 04Standard Test Method forNeutralization of Virucidal Agents in Virucidal EfficacyEvaluations1This standard is issued under the fixed designation E 1482; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year
2、of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers the use, in conjunction withevaluations of the virucidal efficacy, of disinfectant solutio
3、ns orpressurized disinfectant spray products intended for use oninanimate nonporous environmental surfaces or for otherspecial applications. The test method may be employed with allviruses and host systems.1.2 This test method should be performed only by personstrained in microbiology and virology.1
4、.3 This test method utilizes gel filtration technology. Theeffectiveness of the test method is dependent on the ratio of gelbed volume to sample size and uniformity in the preparation ofcolumns and centrifugation conditions. The effectiveness ofthis test method is maximized by investigator practice
5、andexperience with gel filtration techniques.1.4 This test method will reduce, but not necessarily elimi-nate, disinfectant toxicity while preserving the titer of inputvirus.1.5 The values stated in SI units are to be regarded as thestandard.1.6 This standard does not purport to address all of thesa
6、fety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2E 1052 Test Method for Efficac
7、y of Antimicrobial AgentsAgainst Viruses in SuspensionE 1053 Test Method for Efficacy of Virucidal Agents In-tended for Inanimate Environmental Surfaces3. Summary of Test Method3.1 After the exposure of a virus to a disinfectant, thevirus-disinfectant suspension is applied to a column of Sepha-dex3L
8、H60-120, or Sephacryl S-1000 Superfine. The column isplaced in a centrifuge and centrifuged to separate the virusfrom the disinfectant by gel filtration. The filtrate (the columnflow-through that contains the virus) is assayed in the appro-priate host system. The untreated virus control suspension i
9、ssimilarly gel filtered, and the virus titer of the filtrate isdetermined by assay of infectivity. The residual cytotoxicity ofthe disinfectant is determined by gel filtration of the disinfec-tant control under the same conditions. Results for the virusinactivation and disinfectant cytotoxicity of g
10、el filtrates arerecorded in the same manner as described in Test MethodsE 1052 and E 1053. The gel filtration procedures described inthis test method are a modification of the method of Blackwelland Chen.44. Significance and Use4.1 This test method is to be used for the removal ofvirucidal agents fr
11、om agent-virus mixtures, or from agent-neutralizer-virus mixtures, after the contact period and beforethe inoculation of these mixtures into host systems for assay ofinfectivity.4.2 The purpose of the test method is to reduce the concen-tration of agents and neutralizers in order to permit theevalua
12、tion of viral infectivity at dilutions that would otherwisebe toxic to the host.4.3 The test method is applicable to the testing of liquid andpressurized disinfectant products.4.4 This test method is compatible with organic soil loads,hard water, disinfectants containing organic solvents, andchemica
13、l neutralizers.5. Reagents and Materials5.1 Reagents:1This test method is under the jurisdiction of ASTM Committee E35 onPesticides and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.15 on Antimicrobial Agents.Current edition approved Oct. 1, 2004. Published October 2
14、004. Originallyapproved in 1992. Last previous edition approved in 2004 as E 1482 - 92 (2004).2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document
15、 Summary page onthe ASTM website.3Sephadex is a registered trademark of Amersham Biosciences.4Blackwell, H. H., and Chen, J. H. S., “Effects of Various Germicidal Chemicalson H.EP.2 Cell Culture and Herpes simplex Virus,” Journal of the AOAC, Vol 53,1970, pp. 12291236.1Copyright ASTM International,
16、100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.5.1.1 Purity of ReagentsReagent grade chemicals shall beused in all tests. Unless otherwise indicated, it is intended thatall reagents shall conform to the specifications of the Commit-tee on Analytical Reagents of t
17、he American Chemical Society,where such specifications are available.5Other grades may beused, provided it is first ascertained that the reagent is ofsufficiently high purity to permit its use without lessening theaccuracy of the determination.5.1.2 Phosphate Buffered Saline (PBS) (see Dulbecco andV
18、ogt6).5.1.3 Sterile Distilled or Deionized Water.5.2 Sephadex Gel Filtration:5.2.1 Sephadex LH-60-120, compatible with organic sol-vents. (Sephacryl S-1000 Superfine may be substituted.)5.2.2 Syringe, 5 cc or 10 cc, disposable.5.2.3 Glass Wool, sterilized.5.2.4 Centrifuge Tube, 15 and 20 mL, conical
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