1、Designation: E 2360 04Standard Specification forCryptolaemus montrouzieri Mulsant (Coleoptera:Coccinellidae)1This standard is issued under the fixed designation E 2360; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of la
2、st revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers information on and the testmethod for determining purity, sex ratio, and number of adultsin s
3、hipments of the predatory beetle Cryptolaemus montrouz-ieri.2. Referenced Documents2.1 ASTM Standards:2E 2200 Specification for Information Included With Pack-aging of Multi-cellular Biological Control Organisms3. Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 carriershredded pa
4、per.3.1.2 life stage when shippedadult.3.1.3 name of productCryptolaemus montrouzieri Mul-sant.3.1.4 preferred host preymealybugs.4. Classification4.1 PhylumArthropoda.4.2 ClassInsecta.4.3 OrderColeoptera.4.4 FamilyCoccinellidae.4.5 GenusCryptolaemus.4.6 Speciesmontrouzieri.TEST METHODDETERMINING TH
5、E PURITY, SEXRATIO, AND NUMBER OF ADULTS IN SHIPMENTSOF C. montrouzieri5. Summary of Test Method5.1 This test method describes a method of counting thenumber of C. montrouzieri beetles packaged in a carrier.5.2 The number of C. montrouzieri will be determined byexamining a minimum of three units and
6、 determining the meanvalue. In each container, live contaminants will be identifiedand recorded.6. Significance and Use6.1 The efficacy of mealybug control by C. montrouzieridepends on accurate release numbers of beetles, presence ofmale and female beetles, and absence of live product contami-nants.
7、 Accurate packaging and maintenance of purity andviability of C. montrouzieri shipments is, therefore, essentialfor the effective management of the target pest. This testmethod is intended for use by producers and end-users of thespecified biological control agent. It is complementary to thequality
8、guidelines for Cryptolaemus montrouzieri3.7. Materials7.1 Dissecting microscope or headband magnifier (7 to103).7.2 30 DR snap-top plastic vial.7.3 Pharmaceutical pill counting tray.7.4 Fine-pointed No. 5/0 paint brush.8. Test Unit8.1 A single container with C. montrouzieri is considered atest unit.
9、 A minimum of three containers per shipment will berandomly selected from the shipping box.9. Pre-Test Conditions9.1 If samples must be held before testing, keep at 15C,relative humidity 60 to 90 %, for a maximum of 24 h.9.2 Chill beetles at 10C for one hour prior to counting.10. Sampling10.1 Specif
10、y the number of adults that are expected in eachtest unit as indicated on the package before commencing test.11. Counting Procedure and Sex Ratio Determination11.1 Carefully remove any packing material by hand, allow-ing the beetles to drop back into container. If beetles have been1This specificatio
11、n is under the jurisdiction of ASTM Committee E35 onPesticides and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.30 on Natural Multi-Cellular (Metazoan) Biological ControlOrganisms.Current edition approved April 1, 2004. Published April 2004.2For referenced ASTM stan
12、dards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Developed by the International Organization of Biological Control and pub-lished in L
13、enteren, J. C. van (ed.), 2003.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.shipped in a large container, it is advisable to transfer thebeetles to a snap-top vial or bottle for ease in handling. Gentlytap a portion of the beetles
14、 onto the counting tray andimmediately start counting beetles. Record the count, thenbrush the counted beetles into the pill tray reservoir. Store thecounted beetles in a second container with a snap-on lid.Repeat the process until all the beetles are counted. Whilecounting the beetles, record the p
15、resence of any live arthropodsother than adult Cryptolaemus beetles. Since the beetles start towarm during the counting process, the sample container mustbe repeatedly tapped on a hard surface to cause the beetles todrop to the bottom of container to prevent escape. Forquantities greater than 500 be
16、etles, sub-sampling 10 % byweight or volume is recommended. For determining sex ratio,beetles can be examined chilled or dead. The ventral surface ofeach beetle is examined and the sex recorded. The sex isdetermined by the color of the anterior femur: orange for malesand black for females. Count num
17、ber of females and males ina minimum sample size of 100 beetles.12. Calculations and Interpretation of Results12.1 The following formula is used to estimate the meannumber of adults per test unit:12.1.1 Estimation of mean number of adults released pertest unit:x 5Snwhere:x = mean number of adults pr
18、oduced per test unit,S = sum of counts of each test unit, andn = total number of samples examined.12.2 Interpretation of ResultsThe mean of the three testunits should be equal to or greater than the number specified onthe package. Sex ratio should be equal to or greater than 40 %females. No contamin
19、ants should be present except acceptablefood source such as sterile or frozen moth eggs.13. Precision and Bias13.1 The precision and bias of this test method has not beendetermined.14. Keywords14.1 Cryptolaemus montrouzieri; mealybugs; predatorybeetleASTM International takes no position respecting t
20、he validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard
21、is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International H
22、eadquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This
23、standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).E2360042
