1、Designation: E2360 04 (Reapproved 2010)Standard Specification forCryptolaemus montrouzieri Mulsant (Coleoptera:Coccinellidae)1This standard is issued under the fixed designation E2360; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision
2、 the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers information on and the testmethod for determining purity, sex ratio, and numbe
3、r of adultsin shipments of the predatory beetle Cryptolaemus montrouz-ieri.1.2 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.2. Referenced Documents2.1 ASTM Standards:2E2200 Specification for Information Included with Packag-i
4、ng of Multi-Cellular Biological Control Organisms33. Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 carriershredded paper.3.1.2 life stage when shippedadult.3.1.3 name of productCryptolaemus montrouzieri Mul-sant.3.1.4 preferred host preymealybugs.4. Classification4.1 PhylumArth
5、ropoda.4.2 ClassInsecta.4.3 OrderColeoptera.4.4 FamilyCoccinellidae.4.5 GenusCryptolaemus.4.6 Speciesmontrouzieri.TEST METHODDETERMINING THE PURITY, SEXRATIO, AND NUMBER OF ADULTS IN SHIPMENTSOF C. montrouzieri5. Summary of Test Method5.1 This test method describes a method of counting thenumber of
6、C. montrouzieri beetles packaged in a carrier.5.2 The number of C. montrouzieri will be determined byexamining a minimum of three units and determining the meanvalue. In each container, live contaminants will be identifiedand recorded.6. Significance and Use6.1 The efficacy of mealybug control by C.
7、 montrouzieridepends on accurate release numbers of beetles, presence ofmale and female beetles, and absence of live product contami-nants. Accurate packaging and maintenance of purity andviability of C. montrouzieri shipments is, therefore, essentialfor the effective management of the target pest.
8、This testmethod is intended for use by producers and end-users of thespecified biological control agent. It is complementary to thequality guidelines for Cryptolaemus montrouzieri4.7. Materials7.1 Dissecting microscope or headband magnifier (7 to103).7.2 30 DR snap-top plastic vial.7.3 Pharmaceutica
9、l pill counting tray.7.4 Fine-pointed No. 5/0 paint brush.8. Test Unit8.1 A single container with C. montrouzieri is considered atest unit. A minimum of three containers per shipment will berandomly selected from the shipping box.9. Pre-Test Conditions9.1 If samples must be held before testing, keep
10、 at 15C,relative humidity 60 to 90 %, for a maximum of 24 h.1This specification is under the jurisdiction of ASTM Committee E35 onPesticides and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.30 on Natural Multi-Cellular (Metazoan) Biological ControlOrganisms.Current
11、edition approved Oct. 1, 2010. Published November 2010. Originallyapproved in 2004. Last previous edition approved in 2004 as E2360 04. DOI:10.1520/E2360-04R10.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of
12、 ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Withdrawn. The last approved version of this historical standard is referencedon www.astm.org.4Developed by the International Organization of Biological Control and pub-lished in Lenteren, J. C. van (
13、ed.), 2003.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.9.2 Chill beetles at 10C for one hour prior to counting.10. Sampling10.1 Specify the number of adults that are expected in eachtest unit as indicated on the package before co
14、mmencing test.11. Counting Procedure and Sex Ratio Determination11.1 Carefully remove any packing material by hand, allow-ing the beetles to drop back into container. If beetles have beenshipped in a large container, it is advisable to transfer thebeetles to a snap-top vial or bottle for ease in han
15、dling. Gentlytap a portion of the beetles onto the counting tray andimmediately start counting beetles. Record the count, thenbrush the counted beetles into the pill tray reservoir. Store thecounted beetles in a second container with a snap-on lid.Repeat the process until all the beetles are counted
16、 Whilecounting the beetles, record the presence of any live arthropodsother than adult Cryptolaemus beetles. Since the beetles start towarm during the counting process, the sample container mustbe repeatedly tapped on a hard surface to cause the beetles todrop to the bottom of container to prevent
17、escape. Forquantities greater than 500 beetles, sub-sampling 10 % byweight or volume is recommended. For determining sex ratio,beetles can be examined chilled or dead. The ventral surface ofeach beetle is examined and the sex recorded. The sex isdetermined by the color of the anterior femur: orange
18、for malesand black for females. Count number of females and males ina minimum sample size of 100 beetles.12. Calculations and Interpretation of Results12.1 The following formula is used to estimate the meannumber of adults per test unit:12.1.1 Estimation of mean number of adults released pertest uni
19、t:x 5Snwhere:x = mean number of adults produced per test unit,S = sum of counts of each test unit, andn = total number of samples examined.12.2 Interpretation of ResultsThe mean of the three testunits should be equal to or greater than the number specified onthe package. Sex ratio should be equal to
20、 or greater than 40 %females. No contaminants should be present except acceptablefood source such as sterile or frozen moth eggs.13. Precision and Bias13.1 The precision and bias of this test method has not beendetermined.14. Keywords14.1 Cryptolaemus montrouzieri; mealybugs; predatorybeetleASTM Int
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24、andards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).E2360 04 (2010)2
