1、BRITISH STANDARD BS1982-3: 1990 Fungal resistance of panel products made of or containing materials of organic origin Part 3: Methods for determination of resistance to mould or mildew IMPORTANT NOTE. It is essential that this Part of BS1982 is read in conjunction with Part0, which is published sepa
2、rately.BS1982-3:1990 This British Standard, having been prepared under the directionof the Wood PreservationStandards PolicyCommittee, was publishedunder the authorityofthe Board ofBSIandcomes intoeffect on 31October1990 BSI08-1999 First published as BS1982 June1953 Second edition September1968 Thir
3、d edition as BS1982-3 October1990 The following BSI references relate to the work on this standard: Committee reference WPC/10 Draft for comment87/52053DC ISBN 0 580 18441 2 Committees responsible for this British Standard The preparation of this British Standard was entrusted by the Wood Preservati
4、on Standards Policy Committee (WPC/-) to Technical Committee WPC/10, upon which the following bodies were represented: Association of Consulting Scientists British Pest Control Association British Wood Preserving and Damp-proofing Association Chemical Industries Association Department of the Environ
5、ment (Building Research Establishment) Timber Research and Development Association Amendments issued since publication Amd. No. Date CommentsBS1982-3:1990 BSI 08-1999 i Contents Page Committees responsible Inside front cover Foreword ii 1 Scope 1 2 Principle 1 3 Materials and reagents 1 4 Apparatus
6、and facilities 2 5 Sampling 2 6 Test specimens 2 7 Procedure 2 8 Assessment 3 9 Validity of the test 3 10 Test report 3 Appendix A Example of test report including table of results 4 Publications referred to Inside back coverBS1982-3:1990 ii BSI 08-1999 Foreword This Part of BS1982 has been prepared
7、 under the direction of the Wood Preservation Standards Policy Committee. BS1982 was published in1968 as asingle standard including three methods of test. This revision provides a fuller consideration of the possible hazards to organic based panel products and has been divided into Parts to allow ea
8、ch method to be kept up-to-date separately. The following Parts supersede BS1982:1968 which is withdrawn. Part0: Guide to methods for determination; Part1: Method for determination of resistance to wood-rotting Basidiomycetes; Part2: Method for determination of resistance to cellulose-decomposing mi
9、crofungi; Part3: Methods for determination of resistance to mould or mildew. In this Part, two additional test fungi have been added to the corresponding method in the1968 edition. Two different test procedures are now used: MethodL is similar to the test in the1968 edition in which the sample is he
10、ld over a humidifying medium at high relative humidity and Method H, in which the sample is held directly in contact with an agar medium. The extent of attack is now assessed by a scale rating based on the percentage coverage of the sample with mould growth. WARNING. This standard calls for the use
11、of substances and procedures that may be injurious to health if adequate precautions are not taken. It refers only to technical suitability and does not absolve the user from legal obligations relating to health and safety at any stage. The procedures described in this standard are intended to be ca
12、rried out by qualified or other suitably trained and/or supervised personnel. Attention is also drawn to the comments on health and safety in Part0 of this standard and to the caution in clause2 of this Part. Technical Committee38 Durability of wood and wood-based products of the European Committee
13、for Standardization (CEN) has just commenced work, under a mandate from the Commission of the European Economic Community (EEC), on the classification of biological hazards and durability of timber, performance of treated timber, and the performance testing of preservatives. With the publication of
14、European Standards arising from this work, this Part of BS1982 will be amended, revised or withdrawn so as to remove any conflicting aspects. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct applic
15、ation. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pagesi andii, pages1 to4, an inside back cover and a back cover. This standard has been updated (see copyright date) and
16、 may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.BS1982-3:1990 BSI 08-1999 1 1 Scope This Part of BS1982 describes two methods of test for the determination of the resistance of panel products to the growth of surface mould or mildew. The
17、 methods are applicable in particular to building materials required to present a decorative finish including particleboard, plywood and plasterboard. Method L is applicable to situations where the material is exposed to high humidity conditions but without wetting from liquid water (low moisture) a
18、nd method H is applicable to situations where the material is exposed to direct wetting (high moisture) for prolonged periods. The tests described are also applicable to products that have received a preservative treatment. NOTEThe titles of the publications referred to in this standard are listed o
19、n the inside back cover. 2 Principle Test pieces are inoculated with spores of moulds that commonly attack manufactured building materials and are placed in dishes either over a humidifying medium (method L) or in direct contact with an agar medium (method H). The test pieces are then incubated at a
20、 temperature and humidity optimum for the growth of moulds and subsequently examined visually for the presence of mould growth. CAUTION. The test procedures involve handling and working with micro-organisms. It is important that personnel trained in microbiology should perform those parts of the tes
21、t involving handling organisms and infected test specimens. It is essential that such personnel are familiar with the general recommendations on personnel safety given in BS2011-2.2J, in particular National appendix Z, and have appropriate equipment and facilities available. 3 Materials and reagents
22、 3.1 Biological materials 3.1.1 Test fungi 3.1.1.1 Obligatory test fungi. The following fungi shall be included in every test. 3.1.1.2 Optional test fungi. If other mould species are relevant to specific circumstances, these shall be obtained from a recognized culture collection. They shall be used
23、in a parallel test. NOTEPure cultures recently isolated from naturally infected material are also relevant. These should be identified and deposited in a recognized culture collection. 3.1.1.3 Maintenance of strains. The strains shall be maintained and treated in accordance with the instructions of
24、their collection of origin. Cultures shall be used for preparing the spore suspensions when they are between14 and28 days old, preferably between14 and21 days old. NOTECultures less than21 days old may also be used after having been stored, securely stoppered, in a refrigerator at a temperature of6
25、3 C for up to3 months. Cultures from previously unopened containers shall be used to make each batch of suspension and the stoppers shall not be removed until the spore suspension is about to be made. 3.1.2 Reference materials (optional). These shall be panel products or other materials of establish
26、ed performance in practice (see8.3 of BS1982-0:1990). 3.2 Other materials and reagents Water complying with grade3 of BS3978 shall be used throughout. 3.2.1 Humidifying substrate. An hydrated, laminar, aluminium-iron-magnesium silicate 1) , exfoliated to yield particles up to3mm diameter, with a bul
27、k density of80kg/m 3to90kg/m 3 . Particles less than1mm in diameter shall be removed by sieving. 3.2.2 Culture medium. The culture medium shall consist of a mineral salt agar with the following composition in distilled or deionized water. Species Strain no. a Aspergillus versicolor IMI45554 (Vuillem
28、in) Tiroboschi Chaetomium globosum IMI45550 Kunze ex Steudel Cladosporium cladosporioides IMI178517 (Fresenius) de Vries Paecilomyces variotii IMI108007 Bainer Penicillium pinophilum IMI114933 Hedgecock Stachybotrys atra IMI82021 Corda Trichoderma viride IMI45553 Persoon ex S F Gray a The acronym IM
29、I refers to strains held by CAB International Mycological Institute (CMI), Ferry Lane, Kew, Surrey. 1) Vermiculite is suitable. Sodium nitrate (NaNO 3 ) 3.0g/L Potassium dihydrogen 1.0g/L orthophosphate (KH 2 PO 4 ) Magnesium sulphate heptahydrate 0.25g/L (MgSO 4 .7H 2 O) Potassium chloride (KCl) 0.
30、25g/L Agar powder 10.0g/LBS1982-3:1990 2 BSI 08-1999 NOTEMedia having this composition are available from commercial suppliers. 3.2.3 Wetting agent solution. Water containing0.5g/L of a wetting agent based on dioctyl sodium sulphosuccinate shall be used. 4 Apparatus and facilities Ordinary laborator
31、y apparatus and in particular the following are required. 4.1 Culture chamber. A closed but not airtight vessel with a domed lid which allows air circulation but avoids droplets of condensation falling on the test samples. NOTEA500mm 350mm seed propagator with a transparent plastics lid, or a large
32、desiccator with the tap open are both suitable. 4.2 Culture vessels. Sterile90mm plastics or glass petri dishes without lids. 4.3 Incubation chamber. Incubator or room, dark and capable of being controlled at24 1 C and705%r.h. 4.4 Microscope. Equipped for magnification of 10 to 50. 4.5 Hand lens. Ma
33、gnification 10. 5 Sampling A minimum of three replicate sheets of the panel product under test shall be sampled. Ensure that they are clean and as free as possible from contaminants that might otherwise support growth and give misleading results. 6 Test specimens 6.1 Sample specimens Reject from eac
34、h sheet of the panel product undertest (clause5) the300mm nearest to eachedge.Cuta sufficient number of test specimens,40mm 40mm, from each sheet usingthe whole thickness of the product, to provide twotest specimens from each sheet for exposure using each test method. Reject any specimens that show
35、defects such as gaps, knot voids, veneer rupture, or discontinuous adhesion. 6.2 Control specimens A40mm disc of filter paper 2)shall be included in every test to check the virulence of the spore suspension. 6.3 Reference materials (optional) Reference specimens shall be cut from reference materials
36、 as described in6.1. 7 Procedure 7.1 Method L: low moisture test conditions 7.1.1 Preparation of culture chamber Mix a suitable quantity of the humidifying substrate(3.2.1) with3 to4 times its mass of water (avoiding excess free water) and autoclave at115 C for 30 min. Place the prepared medium in t
37、he culture chamber(4.1) to give a depth of10mm. 7.1.2 Installation of test and control specimens Place the specimens in sterile petri dishes(4.2) and place the dishes without lids on the humidifying substrate in the culture chamber. Close the culture chamber and place it in the incubation chamber(4.
38、3) for12h prior to inoculation. 7.1.3 Preparation of spore suspension Gently add10mL of the wetting agent solution(3.2.3) to a prepared subculture of each test strain. Sterilize a platinum or a nichrome wire by heating to red heat in a flame and allowing to cool. Use this wire to gently scrape the s
39、urface of the culture to liberate spores. Agitate the liquid slightly to disperse the spores without detaching mycelial fragments. Gently decant the spore suspensions from all the test fungi into a flask containing a few glass beads(2mm to5mm diameter). Mix the contents of the flask vigorously to br
40、eak up any lumps of spores. Filter the suspension through a sterile cotton or glass wool plug into a clean flask. Use the suspension on the day on which it is prepared and do not store it for future use. 7.1.4 Inoculation of test and control specimens Remove the lid from the culture chamber and even
41、ly spray the face and the edges of each specimen with about0.5mL of the spore suspension(7.1.3) and replace the lid. 7.1.5 Incubation of test and control specimens Place the culture chamber containing the inoculated specimens in the incubation chamber(4.3) and incubate at24 1 C for4 weeks. Check the
42、 humidifying substrate at intervals and add water as necessary to compensate for any drying out that may have occurred. 7.2 Method H: High moisture test conditions 7.2.1 Preparation of culture chamber Use the procedure described in7.1.1. 2) Whatman No.1 filter paper or similar is suitable.BS1982-3:1
43、990 BSI 08-1999 3 7.2.2 Preparation of culture vessels Prepare the mineral salt agar medium(3.2.2) and pour the prepared medium into the petri dishes under aseptic conditions to give a layer3mm to4mm in depth. 7.2.3 Installation of test and control specimens Place the specimens on the agar medium in
44、 the petri dishes, and place the dishes without lids on the humidifying substrate in the culture chamber. Close the culture chamber and place it in the incubation chamber for12h prior to inoculation. 7.2.4 Preparation of spore suspension Use the procedure described in7.1.3. 7.2.5 Inoculation of test
45、 and control specimens Use the procedure described in7.1.4. 7.2.6 Incubation of test and control specimens Place the culture chamber containing the inoculated specimens in the incubation chamber(4.3) and incubate at24 1 C for4 weeks. Check the humidifying substrate at intervals and add water as nece
46、ssary to compensate for any drying out that may have occurred. 8 Assessment For either method, at the end of the4week incubation period remove the culture chamber from the incubation chamber and place it in a suitable safety cabinet or fume cupboard equipped with air extraction facilities. Open the
47、chamber and remove the specimens. Examine the upper face and edges of each specimen using the hand lens(4.5) or the microscope(4.4) as necessary to recognize mould growth. Assess the extent of surface growth of mould and classify it in accordance with the following scale. Calculate the notional mean
48、 rating for all six replicate test specimens. 9 Validity of the test The results shall be accepted as valid provided that all the control specimens have a rating of5. 10 Test report The report shall include at least the following (seeAppendix A for an example): a) the number and date of this Part of
49、 this BritishStandard, i.e.BS1982-3:1990; b) the title of this method; c) the title of the procedure used; d) the name of the applicant; e) the name of the test product and a description including as much of the following information as is available together with the basis, or source of the information: 1) type, e.g.plywood, particleboard; 2) identifying mark(s), e.g.batch number; 3) country of origin and producer; 4) main constituents including for plywood, the species of timber in each veneer; 5) the nature of any bonding agents used; 6) product thick
