1、BRITISH STANDARD CONFIRMED MARCH 1985 BS 3996:1978 Specification for Colour coding for one-mark and graduated pipettes (including requirements for the service performance of the colour coding enamels) UDC 542.3:531.732.004.922BS3996:1978 This British Standard, having been prepared under the directio
2、nof the Laboratory Apparatus Standards Committee,was published underthe authority of the Executive Board on 28 February 1978 BSI 09-1999 First published March 1966 First revision February 1978 The following BSI references relate to the work on this standard: Committee references LBC/8, LBC/27 Draft
3、for comment 71/54684 ISBN 0 580 09929 6 Cooperating organizations The Laboratory Apparatus Standards Committee, under whose direction this British Standard was prepared, consists of representatives from the following Government departments and scientific and industrial organizations: Agricultural Re
4、search Council Association for Science Education British Laboratory Ware Association* British Lampblown Scientific Glassware Manufacturers Association Ltd.* British Pharmacopoeia Commission Chemical Industries Association* Chemical Society Chemical Society, Analytical Division Department of Health a
5、nd Social Security* Department of Industry, Laboratory of The Government Chemist* Department of Industry, National Physical Laboratory Glass Manufacturers Federation* Institute of Petroleum Ministry of Defence Pharmaceutical Society of Great Britain Scientific Instrument Manufacturers Association* S
6、ociety of Chemical Industry* Society of Glass Technology* Standardization of Tar Products Tests Committee The organizations marked with an asterisk in the above list, together with the following, were directly represented on the committee entrusted with the preparation of this British Standard: Asso
7、ciation of Clinical Biochemists British Glass Industry Research Association British Society of Scientific Glassblowers Institute of Medical Laboratory Sciences Royal Institute of Chemistry Amendments issued since publication Amd. No. Date of issue CommentsBS3996:1978 BSI 09-1999 i Contents Page Coop
8、erating organizations Inside front cover Foreword ii Section 1. General 1 Scope and field of application 1 2 References 1 Section 2. Colour coding 3 Coding 1 4 Colours 1 5 Method of marking 1 6 Identification of type 1 graduated pipettes 1 Section 3. Durability 7 Requirements 1 8 Test report on dura
9、bility 1 Appendix A Tests for durability of colour enamels 3 Table 1 Colour coding for one-mark pipettes 2 Table 2 Colour coding for graduated pipettes 2 Publications referred to Inside back coverBS3996:1978 ii BSI 09-1999 Foreword This British Standard, prepared under the direction of the Laborator
10、y Apparatus Standards Committee, specifies a system of colour coding for one-mark pipettes and graduated pipettes, and describes test methods for assessing the service performance of the colour coding enamels. The committee responsible for the first edition, which was published in1966, took into acc
11、ount work in the international field so that the system adopted in the British Standard was identical with that published in the ISO Recommendation ISO/R1769 (now International Standard ISO1769), although the range was restricted to codes for those pipettes which were used in the UnitedKingdom. The
12、growth of international trade now makes it appropriate to include in the present edition the full international range, but each code for pipettes which are not known to be used in the UnitedKingdom is marked inTable 1 andTable 2 with an asterisk. As was originally intended, standard test methods for
13、 assessing the service performance of the enamels have now been developed, and they are described inAppendix A of this edition. These methods also are aligned with those under discussion internationally. The coding system is designed to facilitate sorting of large batches of mixed pipettes, mainly b
14、y unskilled operatives. It is not intended to be memorized, and its use to the analyst is likely to be no more than a help to select quickly several similar pipettes from a mixed batch. Pipettes of different capacities or subdivisions are immediately distinguishable by the code if they are of simila
15、r visual appearance; if they bear the same code they are clearly distinguishable by shape and size. It is not intended to recommend the general adoption of colour coding for pipettes or to make colour coding a mandatory requirement of British Standard specifications for pipettes; if, however, pipett
16、es complying with the requirements of the following British Standards are colour coded: BS696, BS700, BS1428-D4, BS1583, then it is recommended that the codes used should comply with the requirements of this British Standard. A British Standard does not purport to include all the necessary provision
17、s of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pagesi andii, pages1 to4, an inside
18、back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.BS3996:1978 BSI 09-1999 1 Section 1. General 1 Scope and field of application 1.1 This British Standard p
19、rovides a system of coding, by means of coloured bands, for one-mark pipettes for identification of nominal capacities, and for graduated pipettes for identification of nominal capacities and units of subdivision. 1.2 Test methods are described for the assessment of the service performance of the en
20、amels, in terms of whether an enamel will withstand attack under the test conditions described inAppendix A. However, the enamels are not classified by degree of resistance. 2 References The titles of the publications referred to in this standard are listed on the inside back cover. Section 2. Colou
21、r coding 3 Coding The colour coding for one-mark pipettes shall be as detailed inTable 1, and for graduated pipettes shall be as detailed inTable 2. 4 Colours In addition to black and white, five colours are specified in this standard. Variations in the enamels used and in the methods of application
22、 appropriate for pipettes made from different types of glass inevitably result in minor variations of colour, and it is therefore not possible to specify exactly the colours to be used. Nevertheless it is recommended that the colours on the pipettes, other than black and white, should approximate to
23、 the following, which are selected from the series of strong colours in BS381C:1964. 5 Method of marking The code shall take the form of colour bands extending at least150 around the circumference of the pipette, and situated not more than70mm from the top of the pipette and not less than20mm above
24、the nearest graduated line. In the case of pipettes intended for mouth use, the minimum distance from the top of the pipette to the upper edge of the nearest colour band shall be12mm. It is recommended that a single band should be6mm to10mm wide and that in the case of two bands each should be3mm to
25、5mm wide and they should be spaced2mm to3mm apart. 6 Identification of type 1 graduated pipettes If required an additional narrow band of the same colour,1mm to1.5mm wide and placed2mm to3mm above the upper edge of the main coding band, may be used to identify type1 graduated pipettes complying with
26、 the requirements of BS700, i.e. pipettes adjusted for delivery from the zero line down to any graduation line. Section 3. Durability 7 Requirements 7.1 Coloured band(s). The coloured band(s) shall be of fired-on enamel and shall comply with the requirements of7.2 and7.3. 7.2 Resistance to detergent
27、 solutions. A colour coding enamel, when tested by the procedure described inA.4, shall not change in colour, with or without loss of gloss, to the extent that its identity is lost or that it can be confused with any other colour used for colour coding. 7.3 Resistance to acid solutions. A colour cod
28、ing enamel, when tested by the procedure described inA.5, shall not change in colour, with or without loss of gloss, to the extent that its identity is lost or that it can be confused with any other colour used for colour coding. NOTEIt is recommended that, if the pipette is cleaned under drastic co
29、nditions, the colour band(s) should not be immersed in the cleaning solution(s). 8 Test report on durability If a test report on the durability of the colour coding enamels is required, the following minimum information shall be given: a) size and description of the batch or consignment of pipettes
30、from which the sample was taken; b) number of test pieces in the sample; c) colour of the enamels tested; d) results of test for resistance to detergent solutions and of test for resistance to acid solutions; e) date of test. Yellow No. 355 (Lemon) Orange No. 557 (Light Orange) Red No. 538 (Post Off
31、ice Red) Green No. 218 (Grass Green) Blue No. 166 (French Blue)BS3996:1978 2 BSI 09-1999 Table 1 Colour coding for one-mark pipettes Table 2 Colour coding for graduated pipettes Nominal capacity Colour code bands British Standards in which these sizes of pipettes are specified ml 0.001 1 Blue 0.002
32、2 Red 0.003 1 Yellow 0.004 2 Green 0.005 1 White 1428-D4 0.01 1 Orange 1428-D4 0.015 2 Blue 0.02 1 Black 1428-D4 0.025 2 White 0.03 2 Yellow 0.035 2 Black 0.04 2 Red 0.05 1 Green 1428-D4 0.075 2 Orange 0.1 1 Blue 0.15 1 White 0.2 1 Red 0.25 2 Green 0.3 1 Yellow 0.4 2 Red 0.5 2 Black 1583 1 1 Blue 69
33、6-1:1583 2 1 Orange 1583 3 1 Black 1583 4 2 Red 1583 5 1 White 1583 6 2 Orange 7 2 Green 8 1 Blue 9 1 Black 10 1 Red 696-1:1583 15 1 Green 1583 20 1 Yellow 1583 25 1 Blue 1583 30 1 Black 40 1 White 50 1 Red 1583 75 1 Green 100 1 Yellow 150 2 Black a 200 1 Blue 1583 a This code is not known to be use
34、d in the United Kingdom. Nominal capacity Sub- division Colour code bands British Standards in which these sizes of pipettes are specified ml ml 0.01 0.001 1 Blue 0.05 0.001 1 Yellow 0.1 0.001 2 Green 0.005 1 Red 1428-D4 0.01 1 White 0.05 2 Orange 1428-D4 0.125 0.125 2 Yellow a 0.2 0.001 2 Blue 0.00
35、2 2 White 0.01 1 Black 1428-D4 0.1 1 Orange 1428-D4 0.5 0.005 1 Green 0.01 2 Yellow 0.02 2 Red 1428-D4 0.05 2 Black 0.25 2 Green a 1 0.01 1 Yellow 700 0.05 2 Green a 0.1 1 Red 1.5 0.01 2 Red a 2 0.01 2 White 0.02 1 Black 700 0.05 2 Orange a 0.1 1 Green 3 0.01 2 Blue a 5 0.05 1 Red 700 0.1 1 Blue 10
36、0.1 1 Orange 700 15 0.1 2 Green a 20 0.1 2 Yellow 25 0.1 1 White 0.2 1 Green 700 50 0.1 2 Orange a 0.2 1 Black a 100 0.2 1 Red a a These codes are not known to be used in the United Kingdom.BS3996:1978 BSI 09-1999 3 Appendix A Tests for durability of colour enamels A.1 Reagents Analytical grade reag
37、ents shall be used throughout. A.1.1 Water, distilled or deionized. Complying with the requirements of BS3978. A.1.2 Detergent solution. Dissolve50g of tetrasodium pyrophosphate (Na 4 P 2 O 7 ) and5g of sodium dodecylbenzene sulphonate (C 18 H 29 SO 3 Na), in1litre of water. A.1.3 Hydrochloric acid.
38、 2N. A.1.4 Acetone A.2 Apparatus A.2.1 Beaker. 1 litre capacity. A.2.2 Clock glass. Of diameter sufficient to cover the1litre beaker. A.2.3 Thermometer. One complying with the requirements of BS1704 would be suitable. A.2.4 Heating bath. Of about5litres capacity, with suitable heating equipment whic
39、h enables a test solution to be maintained at a constant temperature of80 1 C. A.2.5 Sample holder. Suitable for holding30mm lengths of pipette stem, and made from a material resistant to the test solutions, e.g. polypropylene or platinum wire. A.2.6 Stoppered storage vessels A.3 Sample A.3.1 Prepar
40、ation. Cut test pieces about30mm long from the stems of the pipettes so as to include the colour coding enamel. Wash each test piece three times in separate portions of cold water, and then three times in separate portions of acetone. Finally wipe the test pieces with a soft, dry, lintless cloth, an
41、d transfer them to a stoppered storage vessel unless proceeding immediately with testing. A.3.2 Number of pieces. The number of test pieces in a sample will depend on the size of the batch of pipettes examined, but not more than10test pieces either from the same or different samples shall be immerse
42、d in the same test solution, in order to avoid the possibility of the concentration of dissolved enamel in the solution being high enough to change the conditions of test. A.4 Test procedure for resistance to detergent solutions Heat 700ml of the detergent solution to80 1 C in the beaker using the h
43、eating bath. Suspend the sample with the aid of the sample holder, in the hot test solution, so that the test pieces are freely in contact with the solution on all sides. Cover the beaker with the clock glass. Maintain the temperature of the solution at80 1 C for2h from the time of immersion. After
44、this period, remove the test pieces from the solution, wash them thoroughly with water, wipe with a soft, dry, lintless cloth, and then rinse three times in fresh portions of acetone. Allow to drain dry. A.5 Test procedure for resistance to acid solutions Transfer about 700ml of the cold hydrochlori
45、c acid to the clean beaker and allow to stand until it reaches room temperature (20 5 C). Suspend the sample, with the aid of the sample holder, in the hydrochloric acid so that the test pieces are freely in contact with the acid on all sides, and cover with the clock glass. Allow to stand for1h at
46、room temperature. After this period, remove the test pieces from the acid, wash them thoroughly with water, wipe with a soft, dry, lintless cloth, and then rinse three times in fresh portions of acetone. Allow to drain dry. A.6 Examination of sample At the end of each test, compare the colour of the
47、 enamel on each of the treated pieces with that on a similar test piece which has been prepared in accordance withA.3 and then stored without further treatment. Disregard any loss of gloss in the treated test pieces, but note any change or loss of colour such as might lead to confusion with any othe
48、r colour used in colour coding of pipettes (seeclause4).4 blankBS3996:1978 BSI 09-1999 Publications referred to BS 381C, Colours for specific purposes. BS 696, Gerber method for the determination of fat in milk and milk products. BS 696-1, Apparatus. BS 700, Graduated pipettes (including blowout pip
49、ettes). BS 1428, Microchemical apparatus. BS 1428-D4, Capillary pipettes. BS 1583, One-mark pipettes. BS 1704, General purpose thermometers. BS 3978, Water for laboratory use. ISO 1769, Laboratory glassware Pipettes Colour coding. BS 3996:1978 BSI 389 Chiswick High Road London W4 4AL BSIBritishStandardsInstitution BSI is the independent national body responsible for preparing BritishStandards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions BritishStandards are updated by am
