1、BRITISH STANDARD BS5732:1985 Specification for Glass disposable Pasteur pipettes UDC 615.473:666.11/.28462BS5732:1985 This British Standard, having been prepared under the directionof the Laboratory Apparatus Standards Committee, was published under the authorityof the Board of BSI and comes into ef
2、fect on 31January1985 BSI 10-1999 First edition July 1979 First revision January 1985 The following BSI references relate to the work on this standard: Committee reference LBC/27 Draft for comment82/53535 DC ISBN 0 580 14186 1 Committees responsible for this British Standard The preparation of this
3、British Standard was entrusted by the Laboratory Apparatus Standards Committee (LBC/-) to Technical Committee LBC/27, upon which the following bodies were represented: Association of Clinical Biochemists British Laboratory Ware Association British Lampblown Scientific Glassware Manufacturers Associa
4、tion Ltd Department of Health and Social Security Department of Trade and Industry (Laboratory of the Government Chemist) Department of Trade and Industry (National Weights and MeasuresLaboratory) Glass Manufacturers Federation Institute of Medical Laboratory Sciences Medical Sterile Products Associ
5、ation Ministry of Defence Society of Chemical Industry Amendments issued since publication Amd. No. Date of issue CommentsBS5732:1985 BSI 10-1999 i Contents Page Committees responsible Inside front cover Foreword ii 1 Scope 1 2 Definition 1 3 Dimensions 1 4 Designation 1 5 Construction 1 6 Performan
6、ce 1 7 Cleanliness 1 8 Packaging and marking 2 Figure 1 General form of glass disposable Pasteur pipettes 2 Table 1 Nominal dimensions of glass disposable Pasteur pipettes 2 Publication referred to Inside back coverBS5732:1985 ii BSI 10-1999 Foreword This revision of this British Standard has been p
7、repared under the direction of the Laboratory Apparatus Standards Committee and supersedes BS5732:1979, which is withdrawn. The main change from the previous edition BS5732:1979 is the deletion of the160mm size pipette, which is no longer marketed in the United Kingdom, and has only a very limited r
8、elevance in the export market. Correspondence with International Standards. This British Standard is technically equivalent to ISO7712 “Laboratory glassware Disposable Pasteur pipettes” published by the International Organization for Standardization (ISO), differing only in editorial style and in th
9、e replacement of subjective requirements for the material of construction and for freedom from defects and stress, by the objective requirements in5.1 and by the note to that clause, respectively. A British Standard does not purport to include all the necessary provisions of a contract. Users of Bri
10、tish Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pagesi andii, pages1 and2, an inside back cover and a back cover.
11、 This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.BS5732:1985 BSI 10-1999 1 1 Scope This British Standard specifies requirements for the dimensions, construction, cleanliness, packagi
12、ng and marking for the two most commonly used sizes of glass disposable Pasteur pipettes, i.e.nominal sizes145 and230, representing their nominal overall length, expressed in millimetres. The pipettes are uncalibrated and are intended for the transfer and replicate dispensing of drops of solutions a
13、nd of suspensions of biological materials. NOTEThe title of the publication referred to in this standard is given on the inside back cover. 2 Definition For the purposes of this British Standard the following definition applies. disposable Pasteur pipettes pasteur pipettes intended to be used once o
14、nly, or for one series of replicates and then discarded 3 Dimensions 3.1 Sizes The sizes of the pipettes shall be as given in Table 1. NOTEThe general form of the pipettes is illustrated inFigure 1. 3.2 Tolerances The dimensions of the pipettes in any one batch shall not differ by more than the foll
15、owing tolerances: NOTE 1Actual manufacturing techniques usually result in tolerances tighter than those specified in this clause. NOTE 2The wall thicknesses given in Table 1, if uniform, are considered adequate to provide sufficient strength for the pipettes to withstand normal usage and transport w
16、hen packed as specified in8.1 and8.2. 4 Designation The size of the pipettes shall be designated by a number representing their nominal overall length, expressed in millimetres, i.e.sizes145 and230. 5 Construction 5.1 Material The pipettes shall be made of colourless soda-lime-silica, neutral or bor
17、osilicate glass in accordance with class4 or better of BS3473-2. NOTEThe pipettes should be as free as possible from visible defects, and should be reasonably free from internal stresses. 5.2 Top of pipette The tops of the pipettes shall be finished square with their axes and shall be fire-polished.
18、 5.3 Delivery jet The external diameter of the delivery jet shall taper sharply from the shoulder to form a long fine jet (seeFigure 1) for reaching into test tubes of small internal diameter. The tip of the jet shall be cut square with the axis. 5.4 Constriction A constriction shall be provided fro
19、m the top of the pipette, at the distance specified in Table 1, and shall be adequate to retain a plug of fibrous material such as non-absorbent cotton wool. NOTEIf the pipettes are supplied with plugs, the material and quality of the plugs should be agreed between the purchaser and the vendor. 6 Pe
20、rformance 6.1 Resistance to sterilization When subjected to any of the following procedures, the pipettes shall not be affected so as to bring them outside the requirements of clauses3 and5. a) exposure to dry heat for60 2min at160 1 C; b) exposure to saturated steam, in an autoclave, for at least20
21、min at121 1 C; c) exposure to saturated steam, in an autoclave, for at least3.5min at134 1 C. NOTEThe pipettes should otherwise be suitable for subjection to these procedures. 7 Cleanliness The pipettes shall be supplied clean and free from visible dirt and stains. length: 3% wall thickness: 10%BS57
22、32:1985 2 BSI 10-1999 8 Packaging and marking 8.1 Pipettes shall be packed in cartons each containing preferably250 pipettes, and these cartons may be grouped in an outer carton. 8.2 The packaging shall afford protection against the normal hazards of handling, transport and storage. 8.3 Neither the
23、cartons nor the packing materials shall be made of friable materials so that the pipettes will not be contaminated during transport or handling. 8.4 Each carton of250 pipettes (see8.1) and any outer carton shall be clearly marked with the following information: a) manufacturers name and/or registere
24、d trade mark; b) product description, e.g.disposable Pasteur pipettes, size145; c) number of pipettes in the package; d) batch number or date of manufacture; e) number and date of this British Standard, i.e.BS5732:1985 1) . Table 1 Nominal dimensions of glass disposable Pasteur pipettes 1) Marking B
25、S5732:1985 on or in relation to a product is a claim by the manufacturer that the product has been manufactured to the requirements of the standard. The accuracy of such a claim is therefore solely the manufacturers responsibility. Enquiries as to the availability of third party certification to sup
26、port such claims should be addressed to the Director, Quality Assurance Division, BSI, Maylands Avenue, Hemel Hempstead, Herts HP24SQ for certification marks administered by BSI or to the appropriate authority for other certification marks. Size designation Overall length Body length Body external d
27、iameter Body wall thickness Distance of constriction from top Jet tip diameter external internal A B C mm mm mm mm mm mm mm 145 145 to150 85 to100 6.5 to8 0.5 to0.6 20 to30 1.2 to1.8 0.7 to1.5 230 225 to230 Figure 1 General form of glass disposable Pasteur pipettesBS5732:1985 BSI 10-1999 Publication
28、 referred to BS3473, Chemical resistance of glass used in the production of laboratory glassware. BS3473-2, Method for determination of hydrolytic resistance of glass grains at98 C. BS5732:1985 BSI 389 Chiswick High Road London W4 4AL BSIBritishStandardsInstitution BSI is the independent national bo
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