BS EN 1659-1997 In vitro diagnostic systems - Culture media for microbiology - Terms and definitions《实验室诊断系统 微生物培养基 术语和定义》.pdf

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1、| | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | BRITISH STANDARD BS EN 1659 : 1997 The Eur

2、opean Standard EN 1659 : 1996 has the status of a British Standard ICS 01.040.07; 07.100.10 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW In vitro diagnostic systems Culture media for microbiology Terms and definitionsBS EN 1659 : 1997 This British Standard, having been prep

3、ared under the direction of the Health and Environment Sector Board, was published under the authority of the Standards Board and comes into effect on 15 May 1997 BSI 1997 The following BSI references relate to the work on this standard: Committee reference CH/69 Draft for comment 94/506731 DC ISBN

4、0 580 27395 4 Amendments issued since publication Amd. No. Date Text affected Committees responsible for this British Standard The preparation of this British Standard was entrusted to Technical Committee CH/69, In vitro diagnostic systems, upon which the following bodies were represented: Associati

5、on of Clinical Biochemists Association of Clinical Pathologists BLWA Ltd. (The Association of the Laboratory Supply Industry) British Blood Transfusion Society British In Vitro Diagnostics Association British Society for Antimicrobial Chemotherapy British Society for Haematology Department of Health

6、 Health and Safety Executive Institute of Biomedical Science National Biological Standards Board Public Health Laboratory Service Royal College of PathologistsBS EN 1659 : 1997 BSI 1997 i Contents Page Committees responsible Inside front cover National foreword ii Foreword 2 Text of EN 1659 3ii BSI

7、1997 BS EN 1659 : 1997 National foreword This British Standard has been prepared by Technical Committee CH/69 and is the English language version of EN 1659 : 1996 In vitro diagnostic systems Culture media for microbiology Terms and definitions, published by the European Committee for Standardizatio

8、n (CEN). Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, the EN title page, pages 2 to 4, an inside back cover and a back cover.CEN European Committee for Stan

9、dardization Comite Europe en de Normalisation Europa isches Komitee fu r Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1996 Copyright reserved to CEN members. Ref. No. EN 1659 : 1996 E EUROPEAN STANDARD EN 1659 NORME EUROPE ENNE EUROPA ISCHE NORM November 1996 ICS 01.040.07; 07.10

10、0.10 Descriptors: Medicine, bioassay, microbiological analysis, culture media, vocabulary English version In vitro diagnostic systems Culture media for microbiology Terms and definitions Syste mes de diagnostic in vitro Milieux de culture de microbiologie Termes et de finitions In-vitro-Diagnostik/D

11、iagnostika Kulturmedien fu r die Mikrobiologie Begriffe This European Standard was approved by CEN on 1996-10-19. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any a

12、lteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by tr

13、anslation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxemb

14、ourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.Page 2 EN 1659 : 1996 BSI 1997 Foreword This European Standard has been prepared by Technical Committee CEN/TC 140, ln vitro diagnostic systems, the secretariat of which is held by DIN. This European Standard shall be

15、 given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 1997, and conflicting national standards shall be withdrawn at the latest by May 1997. According to the CEN/CENELEC Internal Regulations, the national standards organizations

16、of the following countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom.Page 3 EN 1659 : 1996 BSI 1997 1 Scope This Eur

17、opean Standard provides terms for different classifications of culture media used in microbiology (bacteriology and mycology). 2 Definitions 2.1 culture medium Formulation of substances, in liquid, semi-solid or in solid form, which contain natural and/or synthetic constituents intended to support t

18、he multiplication, or to preserve the viability, of micro-organisms. NOTE. When used in connection with compound words, this term is often shortened into medium (e.g. enrichment medium). 2.2 Culture media classified by composition 2.2.1 chemically defined culture medium Culture medium consisting of

19、chemically defined constituents (i.e. of known molecular structure and degree of purity) only. 2.2.2 chemically incompletely defined culture medium Culture medium consisting entirely or partly of natural raw materials, the chemical compositions of which are incompletely defined. 2.3 Culture media cl

20、assified by consistency 2.3.1 liquid culture medium Culture medium consisting of an aqueous solution of one or more constituents (e.g. peptone water, nutrient broth). NOTE 1. In some cases, solid particles are added to the liquid culture medium (e.g. Tarrozzi broth, Cooked Meat broth). NOTE 2. Liqui

21、d media in tubes, flasks or bottles are commonly called broth. 2.3.2 solid culture medium and semi-solid culture medium Liquid culture medium containing solidifying materials (e.g. agar-agar, gelatine, etc.) in different concentrations. NOTE. Due to the world-wide use of culture media solidified wit

22、h agar-agar, the shortened term agar is often used synonymously for solid culture media and therefore in connection with nouns, e.g. Endo Agar or Sabouraud agar etc. Solid culture media poured into Petri dishes are commonly called plates. Solid culture media poured into tubes that are kept in slante

23、d positions while solidifying the media are often called slants. 2.4 Culture media classified by intent of use 2.4.1 transport medium Culture medium designed to preserve and maintain the viability of micro-organisms for the time period between sample collection and laboratory processing of the sampl

24、e. NOTE. Transport media usually contain substances that do not permit multiplication of micro-organisms but ensure their preservation (e.g. Stuarts or Amies transport medium). 2.4.2 preservation medium Culture medium designed to preserve and maintain the viability of micro-organisms over an extende

25、d period, to protect them against the adverse influences which may occur during long-term storage and to allow recovery after this period. 2.4.3 resuscitation medium Culture medium enabling stressed and damaged micro-organisms to repair and recover their capacity for normal growth without necessaril

26、y promoting their multiplication. 2.4.4 enrichment medium Predominantly liquid culture medium which, due to its composition, provides particularly favourable conditions for multiplication of micro-organisms. 2.4.4.1 selective enrichment medium Enrichment medium which supports the multiplication of s

27、pecific micro-organisms while inhibiting other micro-organisms (e.g. selenite broth). 2.4.4.2 non-selective enrichment medium Enrichment medium which is not devised to selectively inhibit micro-organisms (e.g. nutrient broth). 2.4.5 isolation medium Solid or semi-solid culture medium which supports

28、growth and/or the formation of colonies of micro-organisms. 2.4.5.1 selective isolation medium Isolation medium which supports growth of specific micro-organisms, while inhibiting other micro-organisms (e.g. Leifson agar, MacConkey agar). 2.4.5.2 non-selective isolation medium Isolation medium which

29、 is not devised to selectively inhibit micro-organisms (e.g. nutrient agar). 2.4.6 differential medium Culture medium which permits the testing of one or more physiological/biochemical characteristics of the micro-organisms for their identification (e.g. Urea medium, Kligler agar). NOTE. Differentia

30、l media which can be used as isolation media are referred to as isolation/differential media (e.g. xylose lysine deoxycholate (XLD) agar). 2.4.7 Identification medium Culture medium designed to produce a specific identification reaction which does not require any further confirmatory test. NOTE. Ide

31、ntification media which can be used as isolation media are referred to as isolation/identification media. 2.4.8 Media with multiple intents of use Certain culture media may be assigned to several categories, e.g. Blood agar is a resuscitation medium according to 2.4.3, an isolation medium according

32、to 2.4.5 and a differential medium according to 2.4.6 used for detection of haemolysis.Page 4 EN 1659 : 1996 BSI 1997 2.5 CuIture media classified according to the form of product 2.5.1 dehydrated medium Culture medium in dry form which is not ready for immediate use (e.g. powders, granules, lyophil

33、ized products). NOTE. Most often, rehydration will make a partially completed medium. 2.5.2 partially completed medium Culture medium which still requires one or more additional working steps before its intended use (e.g. melting, pouring, portioning, supplementing). NOTE. In manufacturing terms, th

34、ese media are often called half- finished or semi-finished. 2.5.3 ready-to-use medium Culture medium which is supplied in containers in ready-to-use form (e.g. Petri dishes or tubes or other carriers).blankBSI 389 Chiswick High Road London W4 4AL | | | | | | | | | | | | | | | | | | | | | | | | | | |

35、 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | BSI British Standards Institution BSI is the independent national body responsible for preparing Br

36、itish Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It i

37、s the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front

38、 cover. Tel: 020 8996 9000. Fax: 020 8996 7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be address

39、ed to Customer Services. Tel: 020 8996 9001. Fax: 020 8996 7001. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been published as British Standards, unless otherwise requested. Information on standards BSI provides a wide range

40、 of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also available which give details on all its products and services. Contact the Information Centre. Tel: 020 8996 7111. F

41、ax: 020 8996 7048. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits contact Membership Administration. Tel: 020 8996 7002. Fax: 020 8996 7001. Copyright Copyright s

42、ubsists in all BSI publications. BSI also holds the copyright, in the UK, of the publications of the international standardization bodies. Except as permitted under the Copyright, Designs and Patents Act 1988 no extract may be reproduced, stored in a retrieval system or transmitted in any form or by

43、 any means electronic, photocopying, recording or otherwise without prior written permission from BSI. This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained. If permission is granted, the terms may include royalty payments or a licensing agreement. Details and advice can be obtained from the Copyright Manager. Tel: 020 8996 7070.

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