1、BRITISH STANDARD BS EN 20-1:1992 Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part 1: Application by surface treatment (laboratory method) The European Standard EN20-1:1992 has the status of a British Standard UDC 674.048.4:620.193.87BSEN20-1:19
2、92 This British Standard, having been prepared under the directionof the Technical Sector Board for Building and Civil Engineering, was published underthe authority of the Standards Board and comes into effect on 15 September 1992 BSI 11-1999 The following BSI references relate to the work on this s
3、tandard: Committee reference B/515 Draft for comment 89/55393 DC ISBN 0 580 20646 7 Cooperating organizations The European Committee for Standardization (CEN), under whose supervision this European Standard was prepared, comprises the national standards organizations of the following countries. Aust
4、ria Oesterreichisches Normungsinstitut Belgium Institut belge de normalisation Denmark Dansk Standardiseringsraad Finland Suomen Standardisoimisliito, r.y. France Association franaise de normalisation Germany Deutsches Institut fr Normung e.V. Greece Hellenic Organization for Standardization Iceland
5、 Technological Institute of Iceland Ireland National Standards Authority of Ireland Italy Ente Nazionale Italiano di Unificazione Luxembourg Inspection du Travail et des Mines Netherlands Nederlands Normalisatie-instituut Norway Norges Standardiseringsforbund Portugal Instituto Portugus da Qualidade
6、 Spain Asociacin Espaola de Normalizacin y Certificacin Sweden Standardiseringskommissionen i Sverige Switzerland Association suisse de normalisation United Kingdom British Standards Institution Amendments issued since publication Amd. No. Date CommentsBSEN20-1:1992 BSI 11-1999 i Contents Page Coope
7、rating organizations Inside front cover National foreword ii Foreword 2 Text of EN 20-1 3 National annex NA (informative) Committees responsible Inside back cover National annex NB (informative) Cross-references Inside back coverBSEN20-1:1992 ii BSI 11-1999 National foreword This Part of BSEN20 has
8、been prepared under the direction of the Technical Sector Board for Building and Civil Engineering and is the English language version of EN20-1 “Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part1: Application by surface treatment (laboratory me
9、thod)”, published by the European Committee for Standardization (CEN). EN20-1 was produced as a result of international discussion in which the UnitedKingdom took an active part. BSEN20-1 supersedes BS5217:1975, which is withdrawn. It is intended that BSEN20 will consist of the following Parts: Part
10、 1: Application by surface treatment (laboratory method); Part 2: Application by impregnation (laboratory method). Part 2 will be identical with EN20-2, which is in preparation. The principal differences between this Part of BSEN20 and BS5217:1975 include: enrichment of the test specimens with a nut
11、rient solution; a change in size of the test specimens from100mm 40mm 15mm to50mm 25mm 15mm; the preservative is applied by pipette to each face instead of dipping each specimen; each test specimen is exposed to only four male and four female insects instead of at least five of each; the use of 2-na
12、phthol has been removed; the minimum time of exposure of the test specimens to the insects is specified; the procedure for the examination of the test specimens has been changed; the conditions for assessing the validity have been changed. CAUTION. Attention is drawn to the Health and Safety at Work
13、 etc. Act1974, and the need for ensuring that the method specified in this Part of BSEN20 is carried out with suitable precautions. The procedure described in this Part of BSEN20 is intended to be carried out by appropriately qualified and experienced persons or other suitably trained and/or supervi
14、sed personnel. Attention is drawn to the precautions given in the introduction,5.2.5 and5.3.4. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does
15、not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, theEN title page, pages 2 to 14, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments
16、 incorporated. This will be indicated in the amendment table on the inside front cover.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 20-1 July 1992 UDC 674.048.4:620.193.87 Together with EN20-2 supersedes EN20:1974 Descriptors: Wood, wood preservatives, pesticides, insecticides, protection ag
17、ainst pests, laboratory tests, determination, effectiveness, prevention, lyctus English version Wood preservatives Determination of the protective effectiveness against Lyctus brunneus (Stephens) Part1:Application by surface treatment (laboratory method) Produits de prservation du bois Dtermination
18、de lefficacit protectrice vis-vis de Lyctus brunneus (Stephens) Partie1: Application par traitement de surface (Mthode de laboratoire) Holzschutzmittel Bestimmung der vorbeugenden Wirkung gegenber Lyctus brunneus (Stephens) Teil1:Oberflchenbehandlung (Laboratoriumsverfahren) This European Standard w
19、as approved by CEN on 1992-07-01. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such natio
20、nal standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and not
21、ified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and UnitedKingdo
22、m. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels CEN 1992 Copyright reserved to CEN members Ref. No. EN 20-1:1992 EEN20-1:1992 BSI 11-1999 2 Foreword This Part of this European Standar
23、d has been prepared by Technical Committee CEN/TC38 “Durability of wood and wood-based products”, the Secretariat of which is held by AFNOR. This Part of EN20 together with EN20-2 replaces EN20:1974. This Part of EN20 is required to enable effectiveness assessments of preservatives which are intende
24、d to be applied by surface treatment. National standards identical to this European Standard shall be published at the latest by 93-01-31 and conflicting national standards shall be withdrawn at the latest by93-01-31. This Part of this EN20 was adopted by CEN in accordance with the CEN/CENELEC Inter
25、nal Regulations. The following countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and UnitedKingdom. Contents Page Foreword 2 Introduction
26、 3 1 Scope 3 2 Normative references 3 3 Definitions 3 4 Principle 3 5 Test materials and apparatus 4 6 Sampling 4 7 Test specimens 4 8 Procedure 5 9 Validity of test 7 10 Expression of results 7 11 Test report 7 Annex A (informative) Example of a test report 9 Annex B (informative) Technique for cul
27、turing Lyctus brunneus 11 Annex C (informative) Principal parasites and predators of Lyctus 13 Annex D (informative) Bibliography 14 Figure B.1 Checking for the presence of sufficient starch content in European oak using Lugol reagent sufficient starch content 11 Figure B.2 Checking for the presence
28、 of sufficient starch content in European oak using Lugol reagent insufficient starch content 12 Figure B.3 Last ventral segment of the abdomen of Lyctus brunneus for the identification of sex 13 Table A.1 Results 10EN20-1:1992 BSI 11-1999 3 Introduction This Part of this EN20 describes a laboratory
29、 method of test which gives a basis for assessment of the protective effectiveness of a wood preservative, when applied as a surface treatment, against Lyctus brunneus. It allows the determination of the concentration at which the product prevents the development of infestation from egg-laying. It c
30、an also be used with formulations ready for use. The species Lyctus brunneus is chosen because of its particular practical relevance and because it can be used easily in laboratory tests. The method can be used with other lyctid species, but the results may not be comparable with those obtained with
31、 Lyctus brunneus. The test specimens are enriched with a defined nutrient solution, before exposure to egg-laying, in order to ensure uniformity of nutrient quality of test specimens between different laboratories. This laboratory method provides one criterion by which the value of a product can be
32、assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When produ
33、cts which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use
34、 of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel. 1 Scope This Part of EN20 specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Lyctus brunneu
35、s (Stephens) when the product is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides, or, organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or, organic water-dispe
36、rsible formulations as supplied or as prepared in the laboratory by dilution of concentrates, or water-soluble materials, for example salts. NOTEThis method may be used in conjuction with ageing procedures, which do not remove the added nutrient. 2 Normative references This European Standard incorpo
37、rates by dated or undated reference, provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this Europe
38、an Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies. ISO 835-1:1981, Laboratory glassware Graduated pipettes Part1: General requirements. ISO 3696:1987, Water for analytical laboratory use Specification a
39、nd test methods. 3 Definitions For the purposes of this Part of EN20, the following definitions apply. 3.1 representative sample a sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier the sponsor of
40、the test 4 Principle Depending on the test being carried out either a set of test specimens of a susceptible wood species is impregnated with a nutrient solution and then surface treated with a solution of the preservative; or if toxic values are to be determined, several sets of test specimens of a
41、 susceptible wood species are impregnated with a nutrient solution and then surface treated with a series of solutions in which the concentration of preservative is ranged in a given progession. The treated test specimens are exposed to adult Lyctus brunneus and the resulting attack compared with th
42、at in untreated controls. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared with that in solvent or diluent treated controls.EN20-1:1992 4 BSI 11-1999 5 Test materials and apparatus 5.1 Biological
43、material Lyctus brunneus (Stephens), insects emerged from cultures not more than48h before use in the test, reared for at least two generations on non-enriched oak or no more than three generations on enriched oak. NOTEThe culturing of Lyctus brunneus requires care in order to obtain a regular suppl
44、y of adults which have not already laid eggs. The culturing technique, which experience has shown to be suitable, is described inAnnex B. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase.
45、NOTEParaffin wax with a setting point of52 C to54 C has been found suitable. 5.2.2 Gelatin, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-tox
46、ic to Lyctus and insoluble in the product under test. NOTESodium carboxymethylcellulose, food grade, has been found suitable. 5.2.4 Water, complying with grade3 of ISO3696. 5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the w
47、ood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION. Do not use benzene or other solvents which pose a health risk. 5.2.6 Peptone, prepared as an enzymatic hydrolysate of meat. 5.2.7 D(+) glucose 5.2.8 Filter paper ordinary quality, medium-fast gr
48、ade 5.2.9 Fine cloth of cotton or linen, with a mesh aperture of less than0,3mm. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (26 1) C, and at relative humidity(75 5)%. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and relative humidity(65 5)%. NOT
49、EThe conditioning of specimens may be carried out in the laboratory work area (see5.3.4) provided that this has the conditions specified for the conditioning chamber (see5.3.2). 5.3.3 Drying chamber, well ventilated, controlled at(302) C. 5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION. It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvent
