1、BSI Standards PublicationBS EN ISO 10519:2015Rapeseed Determinationof chlorophyll content Spectrometric methodBS EN ISO 10519:2015Incorporating corrigendum October 2015BS EN ISO 10519:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO 10519:2015. It supers
2、edes BS EN ISO 10519:2001 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/307, Oilseeds, animal and vegetable fats and oils and their by-products.A list of organizations represented on this committee can be obtained on request to its secretary.This
3、publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2015. Published by BSI Standards Limited 2015ISBN 978 0 580 92204 6ICS 67.200.20Compliance with a British Standard cannot confer im
4、munity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 October 2015.Amendments/corrigenda issued since publicationDate Text affected31 October 2015 ISO pages correctedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM
5、 EN ISO 10519 September 2015 ICS 67.200.20 Supersedes EN ISO 10519:2000English Version Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015) Graines de colza - Dtermination de la teneur en chlorophylle - Mthode spectromtrique (ISO 10519:2015) Rapssamen - Bestimmung
6、des Chlorophyllgehaltes - Spektrometrisches Verfahren (ISO 10519:2015) This European Standard was approved by CEN on 5 September 2015. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national s
7、tandard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version i
8、n any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
9、Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROP
10、EAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10519:2015 EB
11、S EN ISO 10519:2015EN ISO 10519:2015 (E) 3 European foreword This document (EN ISO 10519:2015) has been prepared by Technical Committee ISO/TC 34 “Foodproducts” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fatsand oils and their by-products - Methods of sampli
12、ng and analysis” the secretariat of which is held byAFNOR. This European Standard shall be given the status of a national standard, either by publication of anidentical text or by endorsement, at the latest by March 2016, and conflicting national standards shallbe withdrawn at the latest by March 20
13、16. Attention is drawn to the possibility that some of the elements of this document may be the subject ofpatent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patentrights. This document supersedes EN ISO 10519:2000. According to the CEN-CENELEC Internal Re
14、gulations, the national standards organizations of thefollowing countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,France, Germany, Greece, Hungary, Iceland, Ireland, It
15、aly, Latvia, Lithuania, Luxembourg, Malta,Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,Turkey and the United Kingdom. Endorsement notice The text of ISO 10519:2015 has been approved by CEN as EN ISO 10519:2015 without any modification. BS EN ISO 1051
16、9:2015EN ISO 10519:2015 (E) 3 European foreword This document (EN ISO 10519:2015) has been prepared by Technical Committee ISO/TC 34 “Foodproducts” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fatsand oils and their by-products - Methods of sampling and analys
17、is” the secret riat of which is held byAFNOR. This European Standard shall be given the status of a national standard, either by publication of anidentical text or by endorsement, at the latest by March 2016, and conflicting nation standards shallbe withdrawn at the latest by March 2016. Attention i
18、s drawn to the possibility that some of the elements of this documen may be the subject ofpatent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patentrights. This document supersedes EN ISO 10519:2000. According to the CEN-CENELEC Internal Regulations, the n
19、ational standards orga izations of thefollowing countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lit
20、huania, Luxembourg, Malta,Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,Turkey and the United Kingdom. Endorsement notice The text of ISO 10519:2015 has been approved by CEN as EN ISO 10519:2015 without any modification. BS EN ISO 10519:2015EN ISO 105
21、19:2015 (E) 3 European fore ord This docu ent (EN ISO 10519:2015) has been prepared by Technical Co it ee ISO/TC 34 “Foodproducts” in col aboration with Technical Co ittee CEN/TC 307 “Oilseeds, vegetable and ani al f tsand oils and their by-products - ethods of sampling and analysis” the secretariat
22、 of which is held byAFNOR. This European Standard shal be given the status of a national standard, either by publication of anidentical text or by endorse ent, at the latest by arch 2016, and conflicting national standards shalbe withdrawn at the latest by March 2016. At ention is dra n to the possi
23、bility that so e of the ele ents of this docu ent may be the subject ofpatent rights. CEN and/or CENELEC shall not be held responsible for identifying any or al such patentrights. This document supersedes EN ISO 10519:2000. According to the CEN-CENELEC Internal Regulations, the national standards or
24、ga izations of thefol owing countries are bound to i plement this European Standard: Austria, Bel iu , Bulgaria,Croatia, Cyprus, Czech Republic, Den ark, Estonia, Finland, For er Yugoslav Republic of Macedonia,France, Ger any, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxe bourg,
25、Malta,Netherlands, Norway, Poland, Portugal, Ro ania, Slovakia, Slovenia, Spain, S eden, S itzerl nd,Turkey and the United Kingdo . Endorse e t notice The text of ISO 10519:2015 has been approved by CEN as EN ISO 10519:2015 wit out any modification. ISO 2015Rapeseed Determination of chlorophyll cont
26、ent Spectrometric methodGraines de colza Dtermination de la teneur en chlorophylle Mthode spectromtriqueINTERNATIONAL STANDARDISO10519Third edition2015-09-15Reference numberISO 10519:2015(E)BS EN ISO 10519:2015ISO 10519:2015(E)ii ISO 2015 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2015, Pub
27、lished in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permis
28、sion can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS EN ISO 10519:2015ISO 10519:2015(E)Fo
29、reword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 15 Reagent . 15.1 Extraction solvent . 16 Apparatus . 17 Sampling 28 Preparation of test sample . 29 Procedure. 39.1 Test portions . 39.2 Extraction 39.3 Determination . 310 Expression of results 311 Precision . 411.1
30、 Repeatability . 411.2 Reproducibility . 412 Test report . 4Annex A (informative) Results of interlaboratory test 5Bibliography 6 ISO 2015 All rights reserved iiiContents PageBS EN ISO 10519:2015ISO 10519:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federati
31、on of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee
32、. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and tho
33、se intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (
34、see www.iso.org/directives). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the do
35、cument will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents). Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms a
36、nd expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 34, Food products, Subcommittee
37、 SC 2, Oleaginous seeds and fruits and oilseed meals.This third edition cancels and replaces the second edition (ISO 10519:1997), of which it constitutes a minor revision.Annex A of this International Standard is for information only.iv ISO 2015 All rights reservedBS EN ISO 10519:2015INTERNATIONAL S
38、TANDARD ISO 10519:2015(E)Rapeseed Determination of chlorophyll content Spectrometric method1 ScopeThis International Standard specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.2 Normative re
39、ferencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
40、ISO 648, Laboratory glassware Single-volume pipettesISO 664, Oilseeds Reduction of laboratory sample to test sampleISO 665, Oilseeds Determination of moisture and volatile matter content3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1chlorophyll
41、 contentmass fraction of substances in the sample contributing to the absorption band at a wavelength near 665 nm, as determined under the operating conditions specified in this International Standard and measured as chlorophyll ANote 1 to entry: The chlorophyll content is expressed in milligrams pe
42、r kilogram.4 PrincipleExtraction of a test portion in a suitable apparatus with a specified extraction solvent. Spectrometric determination of the chlorophyll content of the extracted solution.5 ReagentUse only reagents of recognized analytical grade unless otherwise stated.5.1 Extraction solventTra
43、nsfer to a 500 ml beaker 100 ml of anhydrous ethanol. Add to the contents of the beaker 300 ml of anhydrous iso-octane (2,2,5-trimethylpentane) or anhydrous technical n-heptane or anhydrous petroleum ether (essentially composed of C7hydrocarbons, with a boiling range between 90 C and 100 C).6 Appara
44、tusUsual laboratory apparatus and, in particular, are the following. ISO 2015 All rights reserved 1BS EN ISO 10519:2015ISO 10519:2015(E)6.1 Analytical balance, capable of weighing to the nearest 0,001 g.6.2 Mechanical grinder, blade type, or coffee mill or equivalent.6.3 Mechanical microgrinder (see
45、 Figure 1), comprising stainless-steel tubes of approximately 50 ml volume which can be securely stoppered, stainless-steel ball-bearings ( 16 mm), and an apparatus to shake the securely stoppered tubes horizontally at a frequency of 240 min1, with a horizontal displacement of 3,5 cm, or a commercia
46、l ball mill.Dimensions in millimetresKey1 neoprene or fluorosilicone stopper (No. 3)2 steel casing3 stainless-steel bearings, 16 mmFigure 1 Mechanical microgrinder6.4 Filter paper, medium speed, V-folded.6.5 Spectrometer (preferably with wavelength scanning), suitable for carrying out absorbance mea
47、surements at wavelengths between 600 nm and 700 nm, with a spectral bandwidth of 2 nm.6.6 Optical cells, having a path length of at least 1 cm.6.7 Pipettes, of 30 ml capacity, complying with the requirements of ISO 648, class A, or a repetitive dispenser capable of dispensing 30 ml with an error of
48、less than 1 %.6.8 Culture tubes, of 20 ml capacity, provided with stoppers.7 SamplingSampling is not part of the method specified in this International Standard. A recommended sampling method is given in ISO 542.It is important that the laboratory receive a sample which is truly representative and h
49、as not been damaged or changed during transport and storage.8 Preparation of test samplePrepare a test sample in accordance with ISO 664 from the laboratory sample as received, after separation of impurities.2 ISO 2015 All rights reservedBS EN ISO 10519:2015ISO 10519:2015(E)Dry seeds with a moisture content of greater than 10 % (m/m) for 12 h at 45 C to reduce the moisture level to 10 % (m/m) or less in order to reduce the risk of destroying chlorophyll pigments.Transfer 50 g of the test sample to the mechanical grind
