1、BS ISO12872:2010ICS 67.200.10NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDOlive oils andolive-pomace oils Determinationof the 2-glycerylmonopalmitate contentBS EN ISO 12872:2014Copyright British Standards Institution Provided by IHS under license with BSI - U
2、ncontrolled Copy Not for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 12872:2014ISBN 978 0 580 83969 6Amendments/corrigenda issued since publicationDate Comments30 April 2014 This corrigendum renumbers BS ISO 12872:2010 as BS EN ISO 12872:2014This British Stan
3、dard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2010 The British Standards Institution 2014. Published by BSI Standards Limited 2014National forewordThis British Standard is the UK implementation of EN ISO 12872:2014. It is identical to ISO 12872:2010
4、. It supersedes BS ISO 12872:2010, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/307, Oilseeds, animal and vegetable fats and oils and their by-products.A list of organizations represented on this committee can be obtained on request to its secret
5、ary.The publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunity from legal obligations.Copyright British Standards Institution Provided by IHS under license with BS
6、I - Uncontrolled Copy Not for ResaleNo reproduction or networking permitted without license from IHS-,-,-EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 12872 April 2014 ICS 67.200.10 English Version Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (I
7、SO 12872:2010) Huiles dolive et huiles de grignons dolive - Dtermination de la teneur en 2-glycryl monopalmitate (ISO 12872:2010)Olivenle und Oliventresterle - Verfahren zur Bestimmung des Prozentanteiles von 2-Glyceryl Monopalmitat in Olivenlen (ISO 12872:2010) This European Standard was approved b
8、y CEN on 11 April 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standa
9、rds may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and not
10、ified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, I
11、celand, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Ma
12、nagement Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 12872:2014 ECopyright British Standards Institution Provided by IHS under license with BSI - Uncontrolled Copy Not for Resa
13、leNo reproduction or networking permitted without license from IHS-,-,-BS ISO 12872:2010ISO 12872:2010(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are
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18、blished in Switzerland ii ISO 2010 All rights reservedEN ISO 12872:2014 (E) 3 Foreword The text of ISO 12872:2010 has been prepared by Technical Committee ISO/TC 34 “Food products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 12872:2014 by Technical C
19、ommittee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement
20、, at the latest by October 2014, and conflicting national standards shall be withdrawn at the latest by October 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying
21、 any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Re
22、public of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 12872:2010 has been
23、approved by CEN as EN ISO 12872:2014 without any modification. BS EN ISO 12872:2014EN ISO 12872:2014 (E)Copyright British Standards Institution Provided by IHS under license with BSI - Uncontrolled Copy Not for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 12872:2
24、010ISO 12872:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword iv Introduction.v 1 Scope1 2 Normative references1 3 Terms and definitions .1 4 Principle1 5 Reagents.1 6 Apparatus.2 7 Sampling.3 8 Preparation of the test sample.3 9 Procedure.4 9.1 Preparatory steps 4 9.2 Column chromatog
25、raphy4 9.3 Hydrolysis with pancreatic lipase4 9.4 Preparation of the silylated derivatives and gas chromatography 5 9.5 Gas chromatography 5 10 Expression of results5 11 Precision.6 11.1 Interlaboratory test6 11.2 Repeatability 6 11.3 Reproducibility 6 12 Test report6 Annex A (informative) Chromatog
26、rams 7 Annex B (informative) Results of an interlaboratory test .10 Annex C (informative) Preparation and activity of the lipase.11 Bibliography13 BS ISO 12872:2010ISO 12872:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword ivIntroduction.v1 Scope2 Normative references.3 Terms and defin
27、itions .4 Principle5 Reagents.16 Apparatus.27 Sampling.8 Preparation of the test sample.39 Procedure.4 9.1 Preparatory steps 4 9.2 Column chromatography4 9.3 Hydrolysis with pancreatic lipase4 9.4 Preparation of the silylated derivatives and gas chromatography 5 9.5 Gas chromatography 10 Expression
28、of results511 Precision.6 11.1 Interlaboratory test6 11.2 Repeatability 6 11.3 Reproducibility 12 Test report6A Chromatograms 7B Results of an interlaboratory test .10Annex C (informative) Preparation and activity of the lipase.11Bibliography13 BS EN ISO 12872:2014ISO 12872:2010(E)Copyright British
29、Standards Institution Provided by IHS under license with BSI - Uncontrolled Copy Not for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 12872:2010ISO 12872:2010(E) iv ISO 2010 All rights reservedForeword ISO (the International Organization for Standardization) is a
30、 worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represente
31、d on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are draf
32、ted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Sta
33、ndard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 12872 was prepared
34、by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils. BS ISO 12872:2010ISO 12872:2010(E) ISO 2010 All rights reserved vIntroduction As part of the Trade standard applying to olive oils and olive-pomace oils, the International Olive Council OC) publi
35、shed COI/T.20/Doc. 23:20066. COI/T.20/Doc. 23 was applicable to olive and olive-pomace oils and was used to distinguish between lampante virgin olive oils and crude olive-pomace oils. Olive pomace is the residual paste which still contains a variable amount of water and oil after pressing or centrif
36、uging. In 2008, the IOC submitted the document to ISO/TC 34/SC 11 for adoption as an International Standard. BS EN ISO 12872:2014ISO 12872:2010(E)Copyright British Standards Institution Provided by IHS under license with BSI - Uncontrolled Copy Not for ResaleNo reproduction or networking permitted w
37、ithout license from IHS-,-,-BS ISO 12872:2010INTERNATIONAL STANDARD ISO 12872:2010(E) ISO 2010 All rights reserved 1Olive oils and olive-pomace oils Determination of the 2-glyceryl monopalmitate content 1 Scope This International Standard specifies a procedure for the determination of the content, a
38、s a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 C). NOTE This International Standard is based on COI/T.20/Doc. 23:20066. 2 Normative references The following referenced documents are indispensable for the applic
39、ation of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 661, Animal and vegetable fats and oils Preparation of test sample 3 Terms and definitions For the purposes of t
40、his document, the following terms and definitions apply. 3.1 2-glyceryl monopalmitate content mass fraction of 2-glyceryl monopalmitate in the monoacylglycerol fraction, determined according to the method specified in this International Standard NOTE The 2-glyceryl monopalmitate content is expressed
41、 as a percentage. 4 Principle The oil, after suitable preparation, is subjected to the action of pancreatic lipase. A partial hydrolysis takes place that is specific for positions 1 and 3 of the triacylglycerol molecule so that 2-monoacylglycerols are obtained as reaction products. The percentage of
42、 2-glyceryl monopalmitate in the monoacylglycerol fraction is determined, after silylation, by capillary gas chromatography. 5 Reagents WARNING Comply with any local regulations which specify the handling of hazardous substances. Technical, organizational and personal safety measures shall be follow
43、ed. During the analysis, unless otherwise stated, use only reagents of recognized analytical grade, and distilled or demineralized water or water of equivalent purity. BS EN ISO 12872:2014ISO 12872:2010(E)Copyright British Standards Institution Provided by IHS under license with BSI - Uncontrolled C
44、opy Not for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 12872:2010ISO 12872:2010(E) 2 ISO 2010 All rights reserved5.1 Silica gel, with a particle size of 0,063 mm to 0,200 mm (70/280 mesh), prepared as follows: put the silica gel into a porcelain cup, dry in an
45、oven at 160 C for 4 h, then cool at ambient temperature in a desiccator. Add a volume of water equivalent to 5 % of the mass of the silica gel as follows: weigh 152 g of silica gel into a 500 ml Erlenmeyer flask, add 8 g of water, stopper and homogenize carefully. Leave to settle for at least 12 h b
46、efore using. 5.2 n-Hexane, chromatography grade. 5.3 Isopropanol. 5.4 Isopropanol-water mixture, volume fractions 50 ml/100 ml. 5.5 Pancreatic lipase, activity between 2,0 and 10 lipase units per milligram (see Annex C). 5.6 Buffer solution of tris(hydroxymethyl)aminomethane: prepare an aqueous solu
47、tion (1 mol/l) with pH 8 and mix with concentrated HCl, volume fractions 50 ml/100 ml. 5.7 Sodium cholate, special enzyme grade, aqueous solution, mass fraction 0,1 g/100 g. Use this solution within 15 days of preparation. 5.8 Calcium chloride, aqueous solution, mass fraction 22 g/100 g. 5.9 Diethyl
48、 ether, chromatography grade. 5.10 Elution solvent: mixture of n-hexane-diethyl ether, volume fraction of n-hexane 87 ml/100 ml and of diethyl ether 13 ml/100 ml. 5.11 Sodium hydroxide, aqueous solution, mass fraction 12 g/100 g. 5.12 Phenolphthalein, ethanolic solution, mass concentration 1 g/100 m
49、l. 5.13 Carrier gas: hydrogen or helium, gas chromatography grade. 5.14 Auxiliary gases: hydrogen, free from moisture and organic substances, and synthetic air, gas chromatography grade. 5.15 Silylation reagent: mixture of pyridine, hexamethyldisilazane (HMDS) and trimethylchlorosilane (TMCS); volume fractions: 9 ml/13 ml, 3 ml/
