1、BRITISH STANDARD BS EN ISO 14939:2001 Animal feeding stuffs Determination of carbadox content Method using high-performance liquid chromatography The European Standard EN ISO 14939:2001 has the status of a British Standard ICS 65.120 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT
2、 LAWBS EN ISO 14939:2001 This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 18 October 2001 BSI 18 October 2001 ISBN 0 580 3856
3、9 8 National foreword This British Standard is the official English language version of EN ISO 14939:2001. It is identical with ISO 14939:2001. The UK participation in its preparation was entrusted to Technical Committee AW/10, Animal feeding stuffs, which has the responsibility to: A list of organi
4、zations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standard
5、s Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard
6、does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European de
7、velopments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, EN ISO foreword page, ISO title page, pages ii to iv, pages 1 to 16, an inside back cover and a back cover. The BSI copyright date displayed in this documen
8、t indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsEUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM ENISO14939 August2001 ICS65.120 Englishversion AnimalfeedingstuffsDeterminationofcarbadoxcontent Methodusinghighperformanceliquidchromatography(ISO
9、14939:2001) AlimentsdesanimauxDterminationdelateneuren carbadoxMthodeparchromatographieliquidehaute performance(ISO14939:2001) FuttermittelBestimmungdesCarbadoxgehaltes HochleistungsflssigchromatographischesVerfahren(ISO 14939:2001) ThisEuropeanStandardwasapprovedbyCENon15August2001. CENmembersarebo
10、undtocomplywiththeCEN/CENELECInternalRegulationswhichstipulatetheconditionsforgivingthisEurope an Standardthestatusofanationalstandardwithoutanyalteration.Uptodatelistsandbibliographicalreferencesconcernings uchnational standardsmaybeobtainedonapplicationtotheManagementCentreortoanyCENmember. ThisEu
11、ropeanStandardexistsinthreeofficialversions(English,French,German).Aversioninanyotherlanguagemadebytra nslation undertheresponsibilityofaCENmemberintoitsownlanguageandnotifiedtotheManagementCentrehasthesamestatusasthe official versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,CzechR
12、epublic,Denmark,Finland,France,Germany,Greece, Iceland,Ireland,Italy,Luxembourg,Netherlands,Norway,Portugal,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050Brussels 2001CEN All
13、rightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.ENISO14939:2001E2 ENISO14939:2001(E) Foreword ThetextoftheInternationalStandardISO14939:2001hasbeenpreparedbyTechnical CommitteeISO/TC34“Agriculturalfoodproducts“incollaborationwithTechnicalCommittee CEN/TC327“
14、AnimalfeedingstuffsMethodsofsamplingandanalysis“,thesecretariatof whichisheldbyNEN. ThisEuropeanStandardshallbegiventhestatusofanationalstandard,eitherbypublication ofanidenticaltextorbyendorsement,atthelatestbyFebruary2002,andconflictingnational standardsshallbewithdrawnatthelatestbyFebruary2002. A
15、ccordingtotheCEN/CENELECInternalRegulations,thenationalstandardsorganizationsof thefollowingcountriesareboundtoimplementthisEuropeanStandard:Austria,Belgium, CzechRepublic,Denmark,Finland,France,Germany,Greece,Iceland,Ireland,Italy, Luxembourg,Netherlands,Norway,Portugal,Spain,Sweden,Switzerlandandt
16、heUnited Kingdom. Endorsementnotice ThetextoftheInternationalStandardISO14939:2001wasapprovedbyCENasaEuropean Standardwithoutanymodification. ENISO14939:2001 Reference number ISO 14939:2001(E) INTERNATIONAL STANDARD ISO 14939 First edition 2001-08-15 Animal feeding stuffs Determination of carbadox c
17、ontent Method using high- performance liquid chromatography Aliments des animaux Dtermination de la teneur en carbadox Mthode par chromatographie liquide haute performance 1002:93941OSINESBENISO14939:2001 ii ENISO14939:2001 iii Contents Page Foreword.iv 1 Scope 1 2 Normative reference 1 3 Principle1
18、 4 Reagents.2 5 Apparatus .4 6 Sampling.5 7 Preparation of test sample5 8 Procedure .5 8.1 General5 8.2 Preparation of spiked sample.5 8.3 Extraction .6 8.4 Column chromatography7 8.5 HPLC analysis7 9 Confirmation.8 9.1 General8 9.2 Co-chromatography.8 9.3 Diode array detector9 9.4 Post-column deriv
19、atization.10 10 Calculation of results 10 10.1 General10 10.2 Feeding stuffs containing 0,1 mg/kg to 10 mg/kg of carbadox.10 10.3 Feeding stuffs containing 10 mg/kg to 100 mg/kg of carbadox11 10.4 Premixtures containing up to 10 % of carbadox 11 11 Precision.11 11.1 Interlaboratory test 11 11.2 Repe
20、atability.11 11.3 Reproducibility.12 12 Test report 12 Annex A (informative) Flow chart 13 Annex B (informative) Results of interlaboratory test.14 Bibliography16 1002:93941OSINESBENISO14939:2001 iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of nation
21、al standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Internatio
22、nal organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rule
23、s given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the poss
24、ibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 14939 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs. Annexes A and B of this International Standard are for information only. 1002:93941OSINESBENISO14939:2001
