1、16265:2012ICS 13.060.50NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISHSTANDARDWaterqualityDeterminationofthemethyleneblueactivesubstances(MBAS)indexMethodusingcontinuousflowanalysis(CFA)BS EN ISOCopyright European Committee for Standardization Provided by IHS under lice
2、nse with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-National forewordThis British Standard is the UK implementation of EN ISO 16265:2012. It is identical to ISO 16265:2009. It supersedes BS ISO 16265:2009, which is withdrawn.The UK participation in its prep
3、aration was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/2, Physical chemical and biochemical methods.A list of organizations represented on this subcommittee can be obtained on request to its secretary.This publication does not purport to include all the necessary prov
4、isions of a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunity from legal obligations.BS EN ISO 16265:2012This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 August2009 The British
5、 Standards Institution 2012. Published by BSI Standards Limited 2012.Amendments/corrigenda issued since publicationDate Comments 30 April 2012 This corrigendum renumbers BS ISO 16265:2009 as BS EN ISO 16265:2012.ISBN 978 0 580 75430 2Copyright European Committee for Standardization Provided by IHS u
6、nder license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 16265 February 2012 ICS 13.060.50 English Version Water quality - Determination of the methylene blue active substances (MBAS) index - Meth
7、od using continuous flow analysis (CFA) (ISO 16265:2009) Qualit de leau - Mesurage de lindice des substances actives au bleu de mthylne (SABM) - Mthode par analyse en flux continu (CFA) (ISO 16265:2009) Wasserbeschaffenheit - Bestimmung des Indexes von methylenblauaktiven Substanzen (MBAS) - Verfahr
8、en mittels kontinuierlicher Durchflussanalyse (CFA) (ISO 16265:2009) This European Standard was approved by CEN on 15 January 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national stan
9、dard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in a
10、ny other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Rep
11、ublic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT E
12、UROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16265:2012: ECopyright European Committee for Standardization Prov
13、ided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Foreword The text of ISO 16265:2009 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken
14、over as EN ISO 16265:2012 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by August 2012, and conflicting n
15、ational standards shall be withdrawn at the latest by August 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CE
16、N/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Li
17、thuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 16265:2009 has been approved by CEN as a EN ISO 16265:2012 without any modification. BS EN ISO 16265:2012 EN I
18、SO 16265:2012 (E)ii ISO 2012Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Contents PageForeword viIntroduction.vii1Scope. 12Normative references. 13Interferences . 14Principle.
19、 25Reagents26Apparatus 47Sampling and sample pretreatment58Procedure 59Calculation of the results. 710 Expression of results. 811 Precision 812 Test report.8Annex A (informative) Example of a flow diagram for the determination of MBAS index bycontinuous flow analysis (CFA) 9Annex B (informative) Pre
20、cision data. 10Bibliography. 11BS EN ISO 16265:2012 EN ISO 16265:2012 (E) ISO 2012 iiiCopyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Foreword ISO (the International Organization
21、 for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a tec hnical committee has beenestablished has
22、the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closelywith the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Interna
23、tional Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standardsadopted by the technical committees are circulated to the member bodies for voting. Publication
24、as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
25、ISO 16265 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,chemical and biochemical methods. BS EN ISO 16265:2012 EN ISO 16265:2012 (E) ISO 2012iv Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reprodu
26、ction or networking permitted without license from IHS-,-,-Introduction Methodsusing flow analysis automate wet chemical procedures and are particularly suitable for the processing of many analytes in water in large numbers of samples at a high analysis frequency (up to 100 samples per hour).A diffe
27、rentiation is made between flow injection analysis (FIA) 1, 2 and continuous flow analysis (CFA) 3. Both methods share the feature of an automatic dosa ge of the sample into a flow system (manifold) where theanalytes in the sample react with the reagent solutions on their waythrough the manifold. Th
28、e samplepreparation maybe integrated in the manifold. The reaction product is measured in a flow detector (e.g. a photometer). The detector produces a signal from which the concentration of the parameter is calculated. The MBAS (methylene blue active substances)index is an analytical convention (a m
29、ethod-definedparameter) used for water quality control purposes. It measures surfactants and other substances that reactwith methylene blue under specified conditions. The user should be aware that particular problems could require the specification of additional conditions. BS EN ISO 16265:2012 EN
30、ISO 16265:2012 (E)v ISO 2012Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleN
31、o reproduction or networking permitted without license from IHS-,-,-INTERNATIONAL STANDA RD 1Water quality Determination of the methylene blue activesubstances (MBAS) index Method using continuous flowanalysis (CFA)WARNING Persons using this International Standard should be familiar with normal labo
32、ratorypractice. This standard does not p urport to address all of the safety problems, if any, associated withits use. It is the responsibility of the user to establish appropriate safetyand health practices and toensure compliance with any national regulatory conditions. IMPORTANT It is absolutely
33、essential that tests conducted in accordance with this InternationalStandard be carried out by suitably trained staff.Trichloromethane and methanol waste solutions should be disposed of properly. 1 Scope This International Standard specifies a procedure for the determination of the methylene b lue a
34、ctivesubstances (MBAS)index, in the ranges 0,05mg/l to 0,5 mg/l and 0,5 mg/l to 5,0 mg/l, invarious watersamples (e.g. ground water, drinking water,surface water, waste water and leachates). Anionic surfactantsare the most important substances showing methylene blue activity. This method is therefor
35、e useful forestimating the anionic surfactant content including anionic surfactants with carboxylate groups (e.g. soaps) ofwater. Other types of substance mayalso show methylene blue activityand contribute to the result. On a case-by-case basis, the range of the analysis may be changed and the metho
36、d used for other concentrationranges provided they cover exactly one decade of concentration units.2 NormativereferencesThe following referenced documents are indispensable for the application of this document. For datedreferences, only the edition cited applies. For undated references, the latested
37、ition of the reference document(including anyamendments) applies.ISO 648, Laboratory glassware Single-volume pipettesISO 1042, Laboratory glassware One-mark volumetric flasksISO 3696, Water for analytical laboratory use Specification and test methods3 Interferences The following substances can inter
38、fere with the analysis: Cationic compounds able to form strong ion-pairswith the active substances in methylene blue. Humic acids in concentrations 20 mg/l. Chemicals with a high surface activity (e.g. non methylene blue active surfactants in concentrations 50 mg/l).EN ISO 16265:2012 (E)BS EN ISO 16
39、265:2012 ISO 2012Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-2 Chemicals with a strong reductive potential for the oxidation of methylene blue (e.g. S 2 , 23SO , 223SO ,OCl )
40、. These chemicals shall be removed (e.g. with H2 O 2 ) prior to analysis. High concentrations of inorganic anions (e.g. nitrate, chloride, bromide) may cause positive bias. Chemicals which reactwith anyof the reagents used to form a coloured compound soluble in trichloromethane (CHCl 3 ) (excluded a
41、re methylene blue active substances).Filtration of the sample before analysis is advisable for samples containingparticles larger than 100 m in size. Otherwise the particles in the sample may clog the transport tubes. If filtration isnecessary, significant losses of anionic surfactants due to adsorb
42、ance effects are possible.The absorbance due to the colour of the sample can be compensated for by a blank analysis which omits the methylene blue from the reagents. The responses of the sample with and without reagent addition are determined. The difference between the two responses is used in Equa
43、tions (3) and (4).Samples with an MBAS index concentration 5 mg/l shall be diluted before analysis. 4 Principle The sample is mixed, in a continuously flowing stream, with an alkaline methylene blue solution, formingcoloured ion-pairs with certain types of organic substance (e.g. anionic surfactants
44、) contained in the sample. The ion-pairs are extracted with trichloromethane. The organic phase is treated with an acidic methylene bluesolution and its absorbance determined photometricallyat a wavelength of 650 nm 10 nm. The result is expressed in terms of sodium dodecyl sulfate concentration. 5 R
45、eagentsUnless otherwise stated, use only reagents of recognized analytical grade. Degas reagents if air bubbles appear spontaneously. Unless otherwise specified, degas bypassing heliumgas at 20 l/h through the reagent for 15 min. Add detergent after degassing. Avoid using reagents showing anyturbidi
46、ty, filtering them if necessary. 5.1 Water, complying with grade 1 as defined in ISO 3696.5.2 Sodium hydroxide, NaOH. 5.3 Sodium tetraborate decahydrate, Na2 B 4 O 7 10H2 O.5.4Sodium dihydrogen phosphate monohydrate, NaH 2 PO4 H2 O. 5.5 Potassium dihydrogen phosphate monohydrate , KH 2 PO4 H2 O. 5.6
47、 Sulfuric acid, H 2 SO4 , (H2 SO4 ) = 1,84 g/ml. 5.7 Hydrochloricacid , HCl, (HCl) = 1,18 g/ml. 5.8 Methylene blue , C 16H 18N 3 SCl2H2 O. 5.9 Methanol, CH3 OH. 5.10 Ethanol , C2 H 5 OH, w (C2 H 5 OH) 96 %.5.11 Trichloromethane , CHCl3 . Trichloromethane is stable for 3 months if stored in a dark pl
48、ace. BS EN ISO 16265:2012 EN ISO 16265:2012 (E) ISO 2012Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-3Before use, degas the trichloromethane for 30 min by purgingwith a stream of He or by using an ultrasonic bath. 5.12 Sodium dodecyl sulfate (sodium lauryl sulfate), CH3 (CH 2 ) 11OSO 3 Na. 5.13 Petroleum ether . 5.14 Poly(ethylene glycol) dodecyl ether , HO(CH2 CH2 O)n C 12H 25, aqueous solution, w =
