1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 7827:2012Water quality Evaluation ofthe “ready“, “ultimate“ aerobicbiodegradability of organiccompounds in an aqueousmedium Method by analysisof dissolved organic carbo
2、n(DOC) (ISO 7827:2010)Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 7827:2012 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN IS
3、O 7827:2012.It is identical to ISO 7827:2010. It supersedes BS EN ISO 7827:1996which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee EH/3/5, Biological Methods.A list of organizations represented on this committee can beobtained on request to its secretary.Th
4、is publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 65910 2ICS 13.060.70Compliance with a British Standard cannot confer im
5、munity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 January 2013.Amendments issued since publicationDate Text affectedCopyright European Committee for Standardization Provided by IHS under license with CENNot for Re
6、saleNo reproduction or networking permitted without license from IHS-,-,-EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 7827 December 2012 ICS 13.060.70 Supersedes EN ISO 7827:1995English Version Water quality - Evaluation of the “ready“, “ultimate“ aerobic biodegradability of organic comp
7、ounds in an aqueous medium -Method by analysis of dissolved organic carbon (DOC) (ISO 7827:2010) Qualit de leau - valuation de la biodgradabilit arobie “facile“, “ultime“ des composs organiques en milieu aqueux - Mthode par analyse du carbone organique dissous (COD) (ISO 7827:2010) Wasserbeschaffenh
8、eit - Bestimmung der leichten, vollstndigen aeroben biologischen Abbaubarkeit organischer Stoffe in einem wssrigen Medium - Verfahren mittels Analyse des gelsten organischen Kohlenstoffs (DOC) (ISO 7827:2010) This European Standard was approved by CEN on 8 December 2012. CEN members are bound to com
9、ply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELE
10、C Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the s
11、ame status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembour
12、g, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All
13、rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 7827:2012: ECopyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN
14、 ISO 7827:2012EN ISO 7827:2012 (E) 3 Foreword The text of ISO 7827:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 7827:2012 by Technical Committee CEN/TC 230 “Water analysis” the s
15、ecretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2013, and conflicting national standards shall be withdrawn at the latest by June 2013. Attention is drawn
16、to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 7827:1995. According to the CEN/CENELEC Internal Regulations, the nationa
17、l standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithua
18、nia, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 7827:2010 has been approved by CEN as a EN ISO 7827:2012 without any modification. Copyright European Committee fo
19、r Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 7827:2012ISO 7827:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword iv 1 Scope1 2 Normative references1 3 Terms and definitions .2 4 Princ
20、iple .3 5 Test environment.3 6 Reagents 3 7 Apparatus.4 8 Procedure.5 9 Calculation and expression of results 7 10 Validity of the test8 11 Test report9 Annex A (informative) Typical degradation curve .10 Annex B (informative) Interpretation of results .11 Bibliography13 Copyright European Committee
21、 for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 7827:2012ISO 7827:2010(E) iv ISO 2010 All rights reservedForeword ISO (the International Organization for Standardization) is a worldwide federation
22、 of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee.
23、International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance wit
24、h the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approv
25、al by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 7827 was prepared by Technical Committee
26、 ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. This third edition cancels and replaces the second edition (ISO 7827:1994), which has been technically revised. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or n
27、etworking permitted without license from IHS-,-,-BS EN ISO 7827:2012INTERNATIONAL STANDARD ISO 7827:2010(E) ISO 2010 All rights reserved 1Water quality Evaluation of the “ready”, “ultimate” aerobic biodegradability of organic compounds in an aqueous medium Method by analysis of dissolved organic car
28、bon (DOC) WARNING Persons using this International Standard should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health pract
29、ices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to this International Standard be carried out by suitably trained staff. SAFETY PRECAUTIONS Activated sludge and sewage contain potentially pathogenic organisms.
30、 Therefore take appropriate precautions when handling them. Handle toxic test compounds and those whose properties are unknown with care. 1 Scope This International Standard specifies a method for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of
31、concentrations by aerobic microorganisms. In this context, this International Standard also gives specific definitions for the terms “ready” and “ultimate”. The method applies to organic compounds which are: a) soluble at the concentration used under the conditions of the test dissolved organic carb
32、on (DOC) concentrations of 10 mg/l to 40 mg/l; b) non-volatile or having a negligible vapour pressure under the conditions of the test; c) not significantly adsorbable on glass and activated sludge; d) not inhibitory to the test microorganisms at the concentration chosen for the test. The method is
33、not suitable for waste waters, as they usually contain significant amounts of water-insoluble organic carbon, which is not included in DOC measurements. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the ed
34、ition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 8245, Water quality Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC) ISO 9408, Water quality Evaluation of ultimate aero
35、bic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 7
36、827:2012ISO 7827:2010(E) 2 ISO 2010 All rights reservedISO 9439, Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Carbon dioxide evolution test 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.
37、1 degradation time t2time from the end of the lag time, t1, until the time that about 90 % of the maximum level of biodegradation has been reached NOTE Degradation time is expressed in days. 3.2 inherent biodegradation level of biodegradation achieved which indicates the test compound is unlikely to
38、 be persistent in the environment NOTE See Annex B. 3.3 lag time t1time from the start of the test until 10 % biodegradation has been reached NOTE Lag time is expressed in days. 3.4 maximum level of biodegradation degree of biodegradation of a chemical compound or organic matter in a test above whic
39、h no further biodegradation takes place during the test 3.5 primary biodegradation structural change (transformation) of a chemical compound by microorganisms resulting in the loss of a specific property of that compound 3.6 “ready” biodegradation level of biodegradation achieved under defined condi
40、tions which indicates the test compound is considered likely to degrade rapidly and completely under aerobic aquatic environmental conditions NOTE See Annex B. 3.7 suspended solids activated sludge solid material within activated sludge with a particle diameter of 45 m NOTE The concentration of susp
41、ended solids is obtained by filtration or centrifugation of a known volume of sludge under specified conditions, drying at 105 C, and correcting for the volume of sample. The concentration of suspended solids is expressed in milligrams per litre. 3.8 “ultimate” biodegradation breakdown of a chemical
42、 compound or organic matter by microorganisms to carbon dioxide, water and mineral salts of any other elements present (mineralization), and the production of new biomass Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networkin
43、g permitted without license from IHS-,-,-BS EN ISO 7827:2012ISO 7827:2010(E) ISO 2010 All rights reserved 34 Principle The biodegradation of organic compounds by aerobic microorganisms in a mineral medium is determined by measurement of the DOC concentration. The organic compound is the sole source
44、of carbon in the medium. The concentration of the compound used is such that the initial DOC in the medium is between 10 mg/l and 40 mg/l. If necessary, concentrations greater than 40 mg/l may be used. The test solution is aerated in the dark or diffuse light at 22 C 2 C. Biodegradation is monitored
45、 by measurement of the DOC at the start (day 0), at the end of the test (day 28 or longer if necessary), and at a minimum of three intermediate time intervals. The percentage removal of DOC is calculated at each time interval, and the biodegradability of the organic compound based on these data. Spe
46、cific analysis can give additional information on primary biodegradation. The test is not suitable for compounds which are inhibitory at the concentration used in the test. Inhibitory effects can be determined as specified in 8.3 or by using any other method for determining the inhibitory effect on
47、bacteria of a substance (e.g. ISO 81921). The conditions specified in this International Standard do not necessarily correspond to the optimal conditions allowing the maximum degree of biodegradation to occur. Tests for ready biodegradability have very stringent conditions and a substance which pass
48、es these is considered likely to be rapidly and completely degraded in any aerobic aquatic environmental compartment, especially in waste water treatment plants. For alternative biodegradation methods, see ISO/TR 154626. See Annex B for information on interpretation of results. 5 Test environment Incubation shall take place in the dark or in diffused light in an enclosure which is maintained at 22 C 2 C and which is free from vapours that are toxic to microorganisms. 6 Reagents Use only reagents of recognized analytical grade (where applicable). 6.1 Water, distilled or deminerali
