1、BS EN ISO8381:2008ICS 67.100.99; 67.230NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDMilk-based infantfoods Determinationof fat content Gravimetric method(Reference method)(ISO 8381:2008)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08,
2、Uncontrolled Copy, (c) BSIThis British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 January2009 BSI 2009ISBN 978 0 580 63534 2Amendments/corrigenda issued since publicationDate CommentsBS EN ISO 8381:2008National forewordThis British Standard is the UK
3、implementation of EN ISO 8381:2008. Itsupersedes BS EN ISO 8381:2000 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee AW/5, Chemical analysis of milk and milk products.A list of organizations represented on this committee can be obtained onrequest to its
4、 secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal obligations.Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08
5、, Uncontrolled Copy, (c) BSIEUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 8381October 2008ICS 67.230 Supersedes EN ISO 8381:2000 English VersionMilk-based infant foods - Determination of fat content -Gravimetric method (Reference method) (ISO 8381:2008)Aliments base de lait pour enfants en b
6、as ge -Dtermination de la teneur en matire grasse - Mthodegravimtrique (Mthode de rfrence) (ISO 8381:2008)Suglingsnahrung auf Milchbasis - Bestimmung desFettgehaltes - Gravimetrisches Verfahren(Referenzverfahren) (ISO 8381:2008)This European Standard was approved by CEN on 14 October 2008.CEN member
7、s are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application t
8、o the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the sam
9、e status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Roman
10、ia, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2008 CEN All rights of exploitation in any form and by any means reservedworl
11、dwide for CEN national Members.Ref. No. EN ISO 8381:2008: ELicensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008EN ISO 8381:2008 (E) 3 Foreword This document (EN ISO 8381:2008) has been prepared by Technical Committee ISO/TC 34 “Agricu
12、ltural food products“ in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of sampling and analysis” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by en
13、dorsement, at the latest by April 2009, and conflicting national standards shall be withdrawn at the latest by April 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identi
14、fying any or all such patent rights. This document supersedes EN ISO 8381:2000. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmar
15、k, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 8381:2008 has been approved
16、by CEN as a EN ISO 8381:2008 without any modification. Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008ISO 8381:2008(E) IDF 123:2008(E) ISO and IDF 2008 All rights reserved iiiContents Page Foreword iv Foreword. v 1 Scope 1 2 Norm
17、ative references 1 3 Terms and definitions .1 4 Principle2 5 Reagents.2 6 Apparatus .2 7 Sampling.3 8 Preparation of test sample4 8.1 Liquid products4 8.2 Viscous or pasty products4 8.3 Dried products .4 9 Procedure .4 9.1 Test portion 4 9.2 Blank tests5 9.3 Preparation of fat-collecting vessel.5 9.
18、4 Determination.5 10 Calculation and expression of results.8 10.1 Calculation8 10.2 Expression of results 8 11 Precision.8 11.1 Interlaboratory test 8 11.2 Repeatability.8 11.3 Reproducibility.9 12 Test report 9 Annex A (informative) Notes on procedures 10 Annex B (informative) Alternative procedure
19、 using fat-extraction tubes with siphon or wash-bottle fittings .12 Bibliography 15 Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008ISO 8381:2008(E) IDF 123:2008(E) iv ISO and IDF 2008 All rights reservedForeword ISO (the Internat
20、ional Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has bee
21、n established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical stand
22、ardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for
23、 voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all
24、 such patent rights. ISO 8381IDF 123 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO and IDF. This third edition of ISO 8381IDF 123 cancels and replaces the sec
25、ond edition (ISO 8381:2000), of which it constitutes a minor revision. Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008ISO 8381:2008(E) IDF 123:2008(E) ISO and IDF 2008 All rights reserved vForeword IDF (the International Dairy Fe
26、deration) is a non-profit organization representing the dairy sector worldwide. IDF membership comprises National Committees in every member country as well as regional dairy associations having signed a formal agreement on cooperation with IDF. All members of IDF have the right to be represented at
27、 the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO in the development of standard methods of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committ
28、ees for voting. Publication as an International Standard requires approval by at least 50 % of the IDF National Committees casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IDF shall not be held responsible for ident
29、ifying any or all such patent rights. ISO 8381IDF 123 was prepared by the International Dairy Federation (IDF) and Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF and ISO. All work was carried out by the Joint ISO-IDF Acti
30、on Team Fat of the Standing Committee on Main components in milk under the aegis of its project leader, Mr G.J. Beutick (NL). This edition of ISO 8381IDF 123 cancels and replaces IDF 123:1988, of which it constitutes a minor revision. Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009
31、 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008INTERNATIONAL STANDARD ISO 8381:2008(E)IDF 123:2008(E) ISO and IDF 2008 All rights reserved 1Milk-based infant foods Determinatio
32、n of fat content Gravimetric method (Reference method) WARNING The use of this International Standard may involve hazardous materials, operations and equipment. This International Standard does not purport to address all the safety problems associated with its use. It is the responsibility of the us
33、er of this International Standard to establish safety and health practices and determine the applicability of regulatory limitations prior to use. 1 Scope This International Standard specifies the reference method for the determination of the fat content of milk-based infant foods. The method is app
34、licable to liquid, concentrated and dried milk-based infant foods with no, or not more than a mass fraction of 5 % (dry matter) of such added matter as starch, dextrin, vegetables, fruit, and meat. NOTE 1 Malto-dextrins without higher molecular mass dextrins, which are often present in infant foods,
35、 do not disturb the Rse-Gottlieb extraction even when present in high concentrations. The method is not applicable to products which do not dissolve completely in ammonia owing to the presence of starch or dextrin at mass fractions of more than a few percent, or to the presence of hard lumps. The me
36、thod is also not applicable to products which contain free fatty acids in significant quantities. The results obtained for these products are too low. NOTE 2 For such products, a method utilizing the Weibull-Berntrop principle is suitable (see ISO 8262-1IDF 124-13). 2 Normative references The follow
37、ing referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 835, Laboratory glassware Graduated pipettes ISO 1042,
38、Laboratory glassware One-mark volumetric flasks ISO 3889IDF 219, Milk and milk products Specification of Mojonnier-type fat extraction flasks ISO 4788, Laboratory glassware Graduated measuring cylinders 3 Terms and definitions For the purposes of this document, the following terms and definitions ap
39、ply. 3.1 fat content of milk mass fraction of substances determined by the procedure specified in this International Standard NOTE The fat content is expressed as a percentage mass fraction. Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO
40、8381:2008ISO 8381:2008(E) IDF 123:2008(E) 2 ISO and IDF 2008 All rights reserved4 Principle An ammoniacal ethanolic solution of a test portion is extracted with diethyl ether and light petroleum. The solvents are removed by distillation or evaporation. The mass of the substances extracted is determi
41、ned. NOTE This is usually known as the Rse-Gottlieb principle. 5 Reagents Use only reagents of recognized analytical grade, unless otherwise specified, and only distilled or demineralized water or water of equivalent purity. The reagents shall leave no appreciable residue when the determination is c
42、arried out by the method specified (see 9.2.2). 5.1 Ammonia solution, containing a mass fraction of NH3of approximately 25 % (20= 910 g/l). NOTE If ammonia solution of this concentration is not available, a more concentrated solution of known concentration may be used (see 9.4.2). 5.2 Ethanol (C2H5O
43、H), or ethanol denatured by methanol, containing a volume fraction of ethanol of at least 94 %. (See Clause A.5.) 5.3 Congo red solution Dissolve 1 g of Congo red (C32H22N6Na2O6S2) in water in a 100 ml one-mark volumetric flask (6.14). Make up to the mark with water. NOTE The use of this solution, w
44、hich allows the interface between the solvent and aqueous layers to be seen more clearly, is optional (see 9.4.4). Other aqueous indicator solutions can be used provided that they do not affect the result of the determination. 5.4 Diethyl ether (C2H5OC2H5), free from peroxides (see Clause A.3), cont
45、aining no more than 2 mg/kg of antioxidants, and complying with the requirements for the blank test (see 9.2.2, Clauses A.1 and A.4). WARNING The use of diethyl ether can lead to hazardous situations. Observe current safety precautions for handling, use, and disposal. 5.5 Light petroleum, with any b
46、oiling range between 30 C and 60 C or, as equivalent, pentane (CH3CH23CH3) with a boiling point of 36 C and complying with the requirements for the blank test (see 9.2.2, Clauses A.1 and A.4). The use of pentane is recommended because of its higher purity and consistent quality. 5.6 Mixed solvent Sh
47、ortly before use, mix equal volumes of diethyl ether (5.4) and light petroleum (5.5). 6 Apparatus WARNING Since the determination involves the use of volatile flammable solvents, all electrical apparatus employed shall comply with legislation relating to the hazards in using such solvents. Usual lab
48、oratory equipment and, in particular, the following. 6.1 Analytical balance, capable of weighing to the nearest 1 mg, with a readability of 0,1 mg. Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 18/05/2009 09:08, Uncontrolled Copy, (c) BSIBS EN ISO 8381:2008ISO 8381:2008(E) IDF 123:2008(E) I
49、SO and IDF 2008 All rights reserved 36.2 Centrifuge, capable of holding the fat-extraction flasks or tubes (6.6) and capable of spinning at a rotational frequency of 500 min1to 600 min1to produce a radial acceleration of 80g to 90g at the outer end of the flasks or tubes. The use of the centrifuge is optional but recommended (see 9.4.7). 6.3 Distillation or evaporation apparatus, for distilling the solvents and ethanol from the boiling or conical flasks, or evaporating from beakers and dishes (see 9.4.14) at
