1、Soil quality Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditionsBS ISO 14239:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06 ISO 2017Soil quality Laboratory incubation systems for measuring the min
2、eralization of organic chemicals in soil under aerobic conditionsQualit du sol Systmes dincubation de laboratoire destins la mesure de la minralisation de produits chimiques organiques dans le sol en conditions arobiesINTERNATIONALSTANDARDISO14239Second edition2017-07Reference numberISO 14239:2017(E
3、)National forewordThis British Standard is the UK implementation of ISO 14239:2017. It supersedes BS 77554.4.4:1997 (dual numbered as ISO 14239:1997), which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee EH/4, Soil quality.A list of organizations represente
4、d on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017ISBN 978 0 580 932
5、50 2ICS 13.080.30Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 September 2017.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDA
6、RDBS ISO 14239:2017 ISO 2017Soil quality Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditionsQualit du sol Systmes dincubation de laboratoire destins la mesure de la minralisation de produits chimiques organiques dans le sol en conditio
7、ns arobiesINTERNATIONAL STANDARDISO14239Second edition2017-07Reference numberISO 14239:2017(E)BS ISO 14239:2017ISO 14239:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication m
8、ay be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of th
9、e requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 14239:2017ISO 14239:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights
10、 reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from eith
11、er ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgISO 14239:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative refer
12、ences 13 Terms and definitions . 14 Methods . 14.1 General requirements . 14.1.1 Soil collection and characterization 24.1.2 Test material 24.1.3 Incubation conditions 24.2 Choice of incubation systems . 24.3 Flow-through system . 44.3.1 Principle 44.3.2 Materials and reagents . 54.3.3 Apparatus and
13、 glassware . 64.3.4 Procedure . 64.4 Soda-lime column system 74.4.1 Principle 74.4.2 Materials and reagents . 84.4.3 Apparatus and glassware . 94.4.4 Procedure . 94.5 Biometer system . 124.5.1 Principle . 124.5.2 Materials and reagents 134.5.3 Apparatus and glassware 144.5.4 Procedure 144.6 Radiores
14、pirometer . 144.6.1 Principle . 144.6.2 Materials and reagents 154.6.3 Apparatus, glass- and plastic-ware .154.6.4 Procedure 154.7 Microradiorespirometer 164.7.1 Principle . 164.7.2 Materials and reagents 164.7.3 Apparatus, and plastic-ware. 164.7.4 Procedure 174.8 Miniaturized respirometer 174.8.1
15、Principle . 174.8.2 Materials and reagents 184.8.3 Apparatus, and plastic-ware . 184.8.4 Procedure 195 Calculation and expression of results 195.1 For unlabelled test materials . 195.2 For 14C-labelled test materials . 206 Test report 20Bibliography .21 ISO 2017 All rights reserved iiiContents PageB
16、S ISO 14239:2017ISO 14239:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body inter
17、ested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
18、 Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO docume
19、nts should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsi
20、ble for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given
21、for the convenience of users and does not constitute an endorsement.For an explanation on the voluntary nature of standards, on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barri
22、ers to Trade (TBT) see the following URL: Foreword - Supplementary informationThis document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods.This second edition cancels and replaces the first edition (ISO 14239:1997), which has been technically revi
23、sed. The main changes are the inclusion of two additional incubation systems.iv ISO 2017 All rights reservedBS ISO 14239:2017ISO 14239:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of prepa
24、ring International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in l
25、iaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC D
26、irectives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility t
27、hat some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent
28、declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the voluntary nature of standards, on the meaning of ISO specific terms and expressions related to co
29、nformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThis document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods
30、.This second edition cancels and replaces the first edition (ISO 14239:1997), which has been technically revised. The main changes are the inclusion of two additional incubation systems.iv ISO 2017 All rights reserved ISO 14239:2017(E)IntroductionThis document describes incubation systems for determ
31、ining the mineralization of organic compounds in soil under aerobic conditions.Mineralization is only one of the parameters which can be used to assess the biodegradation of organic compounds in soil. If mineralization is not extensive, this does not necessarily mean that the test material is not bi
32、odegradable. Material balance studies to assess the production of metabolites, in addition to mineralization studies, provide a comprehensive assessment of biodegradation.It is essential that this document be used in conjunction with ISO 11266, which gives general guidance on the information needed
33、to assess the potential of an organic compound to be degraded in soil.Depending on the aim of the study, it is feasible to use a range of incubation conditions, described below, and different methods of analysis. ISO 2017 All rights reserved vBS ISO 14239:2017Soil quality Laboratory incubation syste
34、ms for measuring the mineralization of organic chemicals in soil under aerobic conditionsWARNING The methods in this document use several materials of a hazardous nature. Due care is necessary in their handling and disposal. In particular, all pertinent national regulations should be complied with.1
35、 ScopeThis document specifies six suitable incubation systems for measuring the rates and extent of mineralization of organic compounds in soil by measurement of carbon dioxide (CO2) evolution. All incubation systems are applicable to soluble or insoluble compounds but choice of system depends on th
36、e overall purposes of the study.This document does not apply to the use of such systems for material balance studies, which are often test-substance specific.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its
37、application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 11266, Soil quality Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditionsI
38、SO 11269-2:2012, Soil quality Determination of the effects of pollutants on soil flora Part 2: Effects of contaminated soil on the emergence and early growth of higher plantsISO 11274, Soil quality Determination of the water-retention characteristic Laboratory methods ISO 18400-206,1)Soil quality Sa
39、mpling Part 206: Guidance on the collection, handling and storage of soil for the assessment of biological functional and structural endpoints in the laboratory3 Terms and definitionsNo terms and definitions are listed in this document.ISO and IEC maintain terminological databases for use in standar
40、dization at the following addresses: ISO Online browsing platform: available at h t t p :/ www .iso .org/ obp IEC Electropedia: available at h t t p :/ www .electropedia .org/ 4 Methods4.1 General requirementsThe following procedures shall be followed, whichever incubation system is selected.1) Unde
41、r preparation. Stage at the time of publication: ISO/DIS 18400-206:2017.INTERNATIONAL STANDARD ISO 14239:2017(E) ISO 2017 All rights reserved 1BS ISO 14239:2017Soil quality Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditionsWARNING The
42、 methods in this document use several materials of a hazardous nature. Due care is necessary in their handling and disposal. In particular, all pertinent national regulations should be complied with.1 ScopeThis document specifies six suitable incubation systems for measuring the rates and extent of
43、mineralization of organic compounds in soil by measurement of carbon dioxide (CO2) evolution. All incubation systems are applicable to soluble or insoluble compounds but choice of system depends on the overall purposes of the study.This document does not apply to the use of such systems for material
44、 balance studies, which are often test-substance specific.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the lates
45、t edition of the referenced document (including any amendments) applies.ISO 11266, Soil quality Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditionsISO 11269-2:2012, Soil quality Determination of the effects of pollutants on soil flora Part 2: Effec
46、ts of contaminated soil on the emergence and early growth of higher plantsISO 11274, Soil quality Determination of the water-retention characteristic Laboratory methods ISO 18400-206,1)Soil quality Sampling Part 206: Guidance on the collection, handling and storage of soil for the assessment of biol
47、ogical functional and structural endpoints in the laboratory3 Terms and definitionsNo terms and definitions are listed in this document.ISO and IEC maintain terminological databases for use in standardization at the following addresses: ISO Online browsing platform: available at h t t p :/ www .iso
48、.org/ obp IEC Electropedia: available at h t t p :/ www .electropedia .org/ 4 Methods4.1 General requirementsThe following procedures shall be followed, whichever incubation system is selected.1) Under preparation. Stage at the time of publication: ISO/DIS 18400-206:2017.INTERNATIONAL STANDARD ISO 1
49、4239:2017(E) ISO 2017 All rights reserved 1 with ISO 11274 (or between 40 % and 60 % max. water holding capacity (WHC measured to 5 %) in accordance with ISO 11269-2:2012, Annex A) Incubation: in the darkThe incubation conditions should be reported in the test report. If they differ from those above, the reasons for changing them should also be reported in the test report.A temperature of (20 2) C has been chosen as a standard for comparative purposes and because it gives re
