1、BS ISO 14715:2010ICS 71.100.60NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDOil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L.This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 30 April2010
2、BSI 2010ISBN 978 0 580 58059 8Amendments/corrigenda issued since publicationDate CommentsBS ISO 14715:2010National forewordThis British Standard is the UK implementation of ISO 14715:2010.The UK participation in its preparation was entrusted to TechnicalCommittee AW/54, Essential oils.A list of orga
3、nizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal obligations
4、.BS ISO 14715:2010Reference numberISO 14715:2010(E)ISO 2010INTERNATIONAL STANDARD ISO14715Second edition2010-02-01Oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L. Huile essentielle de thym thymol, type Espagne Thymus zygis (Loefl.) L. BS ISO 14715:2010ISO 14715:2010(E) PDF discl
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9、mber body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2010 All rights reservedBS ISO 14715:2010ISO 14715:2010(E) ISO 2010 All rights reserv
10、ed iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for whi
11、ch a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all m
12、atters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are cir
13、culated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held respon
14、sible for identifying any or all such patent rights. ISO 14715 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 14715:1999), which has been technically revised. BS ISO 14715:2010BS ISO 14715:2010INTERNATIONAL STANDARD ISO
15、14715:2010(E) ISO 2010 All rights reserved 1Oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L. 1 Scope This International Standard specifies certain characteristics of the essential oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L., with a view to facilitating
16、the assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) appl
17、ies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essentia
18、l oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in
19、 standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of thyme containing
20、 thymol, Spanish type essential oil obtained by steam distillation of the flowering tops of Thymus zygis (Loefl.) L., of the Lamiaceae family, growing mainly in Spain NOTE For information on the CAS number, see ISO/TR 210922. BS ISO 14715:2010ISO 14715:2010(E) 2 ISO 2010 All rights reserved4 Require
21、ments 4.1 Appearance Mobile liquid. 4.2 Colour Yellowish to red. NOTE The essential oil obtained by steam distillation in a stainless steel kettle would have a much lighter colour than that distilled in other materials. The rectification of red thyme leads to the so called “white thyme” with similar
22、 characteristics. 4.3 Odour Characteristic, aromatic, phenolic (thymol), with a slightly spicy base. 4.4 Relative density at 20 C, 2020d Minimum: 0,910. Maximum: 0,937. 4.5 Refractive index at 20 C Minimum: 1,494. Maximum: 1,504. 4.6 Optical rotation at 20 C Between 0 and 6. NOTE Generally laevorota
23、tory. Most frequently impossible to measure due to its colour. 4.7 Miscibility in 80 % (volume fraction) ethanol at 20 C It shall not be necessary to use more than 3 volumes of 80 % (volume fraction) ethanol to obtain a clear solution with 1 volume of essential oil. 4.8 Chromatographic profile Carry
24、 out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained the representative and characteristic components listed in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic
25、 profile of the essential oil. BS ISO 14715:2010ISO 14715:2010(E) ISO 2010 All rights reserved 3Table 1 Chromatographic profile Component Minimum % Maximum % -Thujene 0,2 1,5 -Pinene 0,5 2,5 Myrcene 1,0 2,8 -Terpinene 0,9 2,6 -Terpinene 4,0 11,0 p-Cymene 14,0 28,0 trans-Sabinene hydrate traces 0,5 L
26、inalool 3,0 6,5 Terpinen-4-ol 0,1 2,5 Methyl ether of carvacrol 0,1 1,5 Thymol 37,0 55,0 Carvacrol 0,5 5,5 -Caryophyllene 0,5 2,0 NOTE The chromatographic profile is normative, contrary to the typical chromatograms given for information in Annex A. 4.9 Flashpoint Information on the flashpoint is giv
27、en in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 2020d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6
28、.3 Optical rotation at 20 C See ISO 592. BS ISO 14715:2010ISO 14715:2010(E) 4 ISO 2010 All rights reserved6.4 Miscibility in 80 % (volume fraction) ethanol at 20 C See ISO 875. 6.5 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and
29、 ISO/TR 211. BS ISO 14715:2010ISO 14715:2010(E) ISO 2010 All rights reserved 5Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of thyme, containing thymol, Spanish type Thymus zygis (Loefl.) L. Peak identification Operating conditions 1 -Thujene
30、2 -Pinene 3 Myrcene 4 -Terpinene 5 p-Cymene 6 -Terpinene 7 trans-Sabinene hydrate 8 Linalool 9 Terpinen-4-ol 10 Methyl ether of carvacrol 11 Thymol 12 Carvacrol 13 -Caryophyllene Column: capillary, fused silica, length 50 m, internal diameter 0,30 mm Stationary phase: poly(methylsiloxane) Film thick
31、ness: 0,25 m Oven temperature: temperature programming from 65 C to 220 C at a rate of 2 C/minInjector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0,35 m/s aprox. Split ratio: 1:100 t time Figure A
32、.1 Typical chromatogram taken on an apolar column BS ISO 14715:2010ISO 14715:2010(E) 6 ISO 2010 All rights reservedPeak identification Operating conditions 1 -Thujene 2 -Pinene 3 Myrcene 4 -Terpinene 5 -Terpinene 6 p-Cymene 7 trans-Sabinene hydrate 8 Linalool 9 -Caryophyllene 10 Terpinen-4-ol 11 Met
33、hyl ether of carvacrol 12 Thymol 13 Carvacrol Column: capillary, fused silica, length 25 m, internal diameter 0,20 mm Stationary phase: polyethylene glycol SP-20 0001) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 200 C at a rate of 5 C/minInjector temperature: 240 C
34、Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0,35 m/s aprox. Split ratio: 1:100 t time Figure A.2 Typical chromatogram taken on a polar column 1) SP-20 000 is an example of a suitable product available commercially. T
35、his information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. BS ISO 14715:2010ISO 14715:2010(E) ISO 2010 All rights reserved 7Annex B (informative) Flashpoint B.1 General information For safety reasons, transport
36、companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend a sin
37、gle apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed for certain test equipment is incompatible with the high price of essential oils; as there are different types of equipment which can be us
38、ed for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the request of the interested parties. The equipment with which this value
39、 was obtained has to be specified. For further information, see ISO/TR 110181. B.2 Flashpoint of the essential oil of thyme, containing thymol, Spanish type Thymus zygis (Loefl.) L. The mean value is +60 C. NOTE Value obtained with Setaflash2)equipment. 2) Equipment available commercially. This info
40、rmation is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. BS ISO 14715:2010ISO 14715:2010(E) 8 ISO 2010 All rights reservedBibliography 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint
41、 2 ISO/TR 21092, Essential oils Characterization BS ISO 14715:2010BS ISO 14715:2010ISO 14715:2010(E) ICS 71.100.60 Price based on 8 pages ISO 2010 All rights reserved BS ISO 14715:2010This page has been intentionally left blankBS ISO14715:2010BSI GroupHeadquarters 389Chiswick High Road,London, W4 4A
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