1、BSI Standards PublicationBS ISO 16385:2014Essential oil of molle (Schinus areira L.), Argentinean typeBS ISO 16385:2014 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 16385:2014. The UK participation in its preparation was entrusted to TechnicalCommittee AW/54
2、, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2014.Publis
3、hed by BSI Standards Limited 2014ISBN 978 0 580 82020 5ICS 71.100.60Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 September 2014.Amendments/corrigenda issue
4、d since publicationDate T e x t a f f e c t e dBS ISO 16385:2014 ISO 2014Essential oil of molle (Schinus areira L.), Argentinean typeHuile essentielle de baie rose (Schinus areira L.), type ArgentineINTERNATIONAL STANDARDISO16385First edition2014-09-01Reference numberISO 16385:2014(E)BS ISO 16385:20
5、14ISO 16385:2014(E)ii ISO 2014 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2014All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the
6、 internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb
7、www.iso.orgPublished in SwitzerlandBS ISO 16385:2014ISO 16385:2014(E) ISO 2014 All rights reserved iiiContents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 14.1 Appearance 14.2 Colour 14.3 Odour . 14.4 Relative density at 20 C, d202024.5 R
8、efractive index at 20 C . 24.6 Optical rotation at 20 C 24.7 Miscibility in ethanol 90 % (volume fraction) at 20 C 24.8 Chromatographic profile 24.9 Flashpoint 25 Sampling 26 Test methods . 36.1 Relative density at 20 C, d202036.2 Refractive index at 20 C . 36.3 Optical rotation at 20 C 36.4 Miscibi
9、lity in ethanol 90 % (volume fraction) at 20 C 36.5 Chromatographic profile 37 Packaging, labelling, marking, and storage 3Annex A (informative) Typical chromatogram of the analysis by gas chromatography of the essential oil of molle (Schinus areira L.) Argentinean type . 4Annex B (informative) Flas
10、hpoint . 6Bibliography 7BS ISO 16385:2014ISO 16385:2014(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committee
11、s. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Inter
12、national Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the diff
13、erent types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO sha
14、ll not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document i
15、s information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT)
16、 see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 54, Essential oils.iv ISO 2014 All rights reservedBS ISO 16385:2014ISO 16385:2014(E)IntroductionThis International Standard considers the requirements that the essential oil obtained by
17、water vapor distillation of the fruits of molle (Schinus areira L.) Argentinean type shall meet.It is a natural product with an intraspecific variability characterized by the preponderance of phellandrenes and limonene as major components. The table of essential oil composition reflects this feature
18、. ISO 2014 All rights reserved vBS ISO 16385:2014BS ISO 16385:2014Essential oil of molle (Schinus areira L.), Argentinean type1 ScopeThis International Standard specifies certain characteristics of the essential oil of molle (Schinus areira L.), Argentinean type, intended for facilitating the assess
19、ment of its quality.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (
20、including any amendments) applies.ISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of
21、miscibility in ethanolISO 11024 (all parts), Essential oils General guidance on chromatographic profilesISO/TR 210, Essential oils General rules for packaging, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containers3 Terms and definitionsFor the purpo
22、ses of this document, the following terms and definitions apply.3.1essential oil of molle (Schinus areira L.)essential oil obtained by steam distillation of the fruits of Schinus areira L., of the Anacardiaceae familyNote 1 to entry: For information on CAS number, see ISO/TR 210922.4 Requirements4.1
23、 AppearanceClear mobile liquid.4.2 ColourLight yellow.4.3 OdourFresh and characteristic of phellandrene.INTERNATIONAL STANDARD ISO 16385:2014(E) ISO 2014 All rights reserved 1BS ISO 16385:2014ISO 16385:2014(E)4.4 Relative density at 20 C, d2020Minimum: 0,850Maximum: 0,8854.5 Refractive index at 20 C
24、Minimum: 1,475 0Maximum: 1,488 04.6 Optical rotation at 20 CBetween +30 and +75.4.7 Miscibility in ethanol 90 % (volume fraction) at 20 CIt shall not be necessary to use less than 4,5 volumes or more than 6,5 volumes of ethanol 90 % (volume fraction) to obtain a clear solution with 1 volume of essen
25、tial oil.4.8 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify, in the chromatogram obtained, the representative and characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Tab
26、le 1. This constitutes the chromatographic profile of the essential oil.Table 1 Chromatographic profileComponentMinimum%Maximum%Myrcene 1,0 14,0Phellandrene (+) 35,0 60,0Limonene 10,0 25,0Sabinene n.d.a8,0Terpinen-4-ol n.d.a1,5-Cadinol 0,5 3,0Germacrene D 0,2 0,5-Humulene 0,0 0,5Elemol 0,0 0,5NOTE T
27、he chromatographic profile is normative, contrary to the typical chromatogram given for information in Annex A.aNot detectable.4.9 FlashpointInformation on the flashpoint is given in Annex B.5 SamplingSampling shall be performed in accordance with ISO 212.2 ISO 2014 All rights reservedBS ISO 16385:2
28、014ISO 16385:2014(E)Minimum volume of test sample: 25 ml.NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.6 Test methods6.1 Relative density at 20 C, d2020Determine the relative density in accordance with ISO 279.6.2 Refractive index
29、at 20 CDetermine the refractive index in accordance with ISO 280.6.3 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592.6.4 Miscibility in ethanol 90 % (volume fraction) at 20 CDetermine the miscibility in ethanol in accordance with ISO 875.6.5 Chromatographic profileD
30、etermine the chromatographic profile in accordance with ISO 11024.7 Packaging, labelling, marking, and storageThese items shall be in accordance with ISO/TR 210 and ISO/TR 211. ISO 2014 All rights reserved 3BS ISO 16385:2014ISO 16385:2014(E)Annex A (informative) Typical chromatogram of the analysis
31、by gas chromatography of the essential oil of molle (Schinus areira L.) Argentinean typePeak identification Operating conditions1 -Pinene Column: capillary, fused silica, length 60 m, internal diameter 0,25 mmStationary phase: polyethylene glycol SP-20 000aFilm thickness: 0,25 mOven temperature: tem
32、perature programming from 90 C to 225 C at a rate of 3 C/minInjector temperature: 255 CDetector temperature: 275 CDetector: flame ionization typeCarrier gas: HeliumVolume injected: 0,2 l of a 10 % dilution in ethanolCarrier gas flow rate: 1,87 ml/minSplit ratio: 1/1002 -Pinene3 Sabinene4 Myrcene5 -P
33、hellandrene6 Limonene7 -Phellandrene8 Terpinen-4-ol9 -Humulene10 Germacrene D11 Elemol12 Epi-Cadinol13 -CadinolaSP-20 000 is an example of a suitable product available commercially. This information is given for the convenience of users of this International Standard and does not constitute an endor
34、sement by ISO of this product.Figure A.1 Typical chromatogram taken on a polar column4 ISO 2014 All rights reservedBS ISO 16385:2014ISO 16385:2014(E)Peak identification Operating conditions1 -Pinene Column: capillary, fused silica, length 60 m, internal diameter 0,25 mmStationary phase: 5 % phenyl 9
35、5 % methyl siliconeFilm thickness: 0,25 mOven temperature: temperature programming from 90 C to 225 C at a rate of 3 C/minInjector temperature: 255 CDetector temperature: 275 CDetector: flame ionization typeCarrier gas: HeliumVolume injected: 0,2 l of a 10 % dilution in ethanolCarrier gas flow rate:
36、 1,87 ml/minSplit ratio: 1/1002 Sabinene3 Myrcene4 -Pinene5 -Phellandrene6 Limonene7 -Phellandrene8 Terpinen-4-ol9 -Humulene10 Germacrene D11 Elemol12 Epi-Cadinol13 -CadinolFigure A.2 Typical chromatogram taken on an apolar column ISO 2014 All rights reserved 5BS ISO 16385:2014ISO 16385:2014(E)Annex
37、 B (informative) FlashpointB.1 General informationFor safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flash points of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of
38、 analysis (see ISO/TR 110181) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: There is a wide variation in the chemical composition of essential oils; The volume of the sample needed in certain requirements would be too costly for high priced
39、 essential oils; As there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order t
40、o meet the requirements of the interested parties.The equipment with which this value was obtained has to be specified. For further information see ISO/TR 11018.1B.2 Flashpoint of the essential oil of molleThe mean value is +48 C.NOTE Obtained with Setaflash1)equipment.1) Equipment available commerc
41、ially. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product.6 ISO 2014 All rights reservedBS ISO 16385:2014ISO 16385:2014(E)Bibliography1 ISO/TR 11018:1997, Essential oils General guidance on the determina
42、tion of flashpoint2 ISO/TR 21092, Essential oils Characterization3 IRAM 18608-2:2006, Flavoring products Essential oils Essential oil of molle. Part 2: Schinus areira L ISO 2014 All rights reserved 7BS ISO 16385:2014ISO 16385:2014(E) ISO 2014 All rights reservedICS 71.100.60Price based on 7 pagesThi
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