1、BSI Standards PublicationBS ISO 16928:2014Essential oil of ginger Zingiber officinale RoscoeBS ISO 16928:2014 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 16928:2014. The UK participation in its preparation was entrusted to TechnicalCommittee AW/54, Essentia
2、l oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2014.Published by BSI
3、 Standards Limited 2014ISBN 978 0 580 75223 0ICS 71.100.60Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 April 2014.Amendments/corrigenda issued since public
4、ationDate T e x t a f f e c t e dBS ISO 16928:2014 ISO 2014Essential oil of ginger Zingiber officinale RoscoeHuile essentielle de gingembre Zingiber officinale RoscoeINTERNATIONAL STANDARDISO16928First edition2014-04-15Reference numberISO 16928:2014(E)BS ISO 16928:2014ISO 16928:2014(E)ii ISO 2014 Al
5、l rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2014All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without
6、 prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in Switzerla
7、ndBS ISO 16928:2014ISO 16928:2014(E) ISO 2014 All rights reserved iiiContents PageForeword iv1 Scope .12 Normative references 13 Terms and definitions .14 Requirements 14.1 Appearance 14.2 Colour 14.3 Odour . 14.4 Relative density at 20 C, d202024.5 Refractive index at 20 C . 24.6 Optical rotation a
8、t 20 C 24.7 Chromatographic profile 24.8 Flashpoint 25 Sampling 36 Test methods .36.1 Relative density at 20 C, d202036.2 Refractive index at 20 C .36.3 Optical rotation at 20 C 36.4 Chromatographic profile 37 Packaging, labelling, marking and storage .3Annex A (informative) Typical chromatograms of
9、 the analysis by gas chromatography of the essential oil of ginger (Zingiber officinale Roscoe) .4Annex B (informative) Flashpoint 11Bibliography .12BS ISO 16928:2014ISO 16928:2014(E)ForewordForewordISO (the International Organization for Standardization) is a worldwide federation of national standa
10、rds bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organ
11、izations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its f
12、urther maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/direc
13、tives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the
14、Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related
15、to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 54, Essential oils.iv ISO 2014 All rights reservedBS I
16、SO 16928:2014INTERNATIONAL STANDARD ISO 16928:2014(E)Essential oil of ginger Zingiber officinale Roscoe1 ScopeThis International Standard specifies certain characteristics of the essential oil of ginger (Zingiber officinale Roscoe) cultivated in China, India and West Africa, in order to facilitate a
17、ssessment of its quality. 2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced docu
18、ment (including any amendments) applies.ISO/TR 210, Essential oils General rules for packaging, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Re
19、ference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 11024 (all parts), Essential oils General guidance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following terms and definiti
20、ons apply.3.1essential oil of gingeressential oil obtained by steam distillation of the rhizome of Zingiber officinale Roscoe of the Zingiberaceae family, cultivated principally in China, India and West AfricaNote 1 to entry: For information on the CAS number, see ISO/TR 210922.4 Requirements4.1 App
21、earanceClear mobile liquid.4.2 ColourChina India West AfricaPale yellow to amber Yellow Pale yellow to yellow4.3 OdourCharacteristic of ginger rhizome, with spicy, peppered, lemony odour. ISO 2014 All rights reserved 1BS ISO 16928:2014ISO 16928:2014(E)4.4 Relative density at 20 C, d2020Value China I
22、ndia West AfricaMinimum 0,873 0,872 0,872Maximum 0,885 0,890 0,8924.5 Refractive index at 20 CValue China India West AfricaMinimum 1,486 1,484 1,486Maximum 1,495 1,498 1,4964.6 Optical rotation at 20 CChina India West AfricaBetween 47 and 26 Between 50 and 27 Between 47 and 184.7 Chromatographic pro
23、fileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative and characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chroma
24、tographic profile of the essential oil.Table 1 Chromatographic profileComponentChina India West AfricaMinimum %Maximum %Minimum %Maximum %Minimum %Maximum %-Pinene 1,5 2,5 2,0 6,0 0,1 3,0Camphene 4,5 10,0 5,0 8,0 0,2 12,0Neral n.d.a0,5 0,1 0,4 0,2 2,0Geraniol 0,1 1,0 0,1 0,6 0,1 3,5Geranial n.d.a0,6
25、 0,1 0,6 0,5 3,5-Elemene 0,5 1,5 0,6 1,1 n.d.a1,0ar-Curcumene 5,0 11,0 6,5 9,0 3,0 11,0-Zingiberene 29,0 40,0 35,0 40,0 23,0 45,0-Bisabolene 2,5 9,0 2,5 5,5 3,0 7,0-Sesquiphellandrene 10,0 14,0 11,5 13,5 8,0 17,0NOTE The chromatographic profile is normative, contrary to typical chromatograms given f
26、or information in Annex A.aNot detected.4.8 FlashpointInformation on the flashpoint is given in Annex B.2 ISO 2014 All rights reservedBS ISO 16928:2014ISO 16928:2014(E)5 SamplingSampling shall be performed in accordance with ISO 212.Minimum volume of test sample: 30 ml.NOTE This volume allows each o
27、f the tests specified in this International Standard to be carried out at least once.6 Test methods6.1 Relative density at 20 C, d2020Determine the relative density in accordance with ISO 279.6.2 Refractive index at 20 CDetermine the refractive index in accordance with ISO 280.6.3 Optical rotation a
28、t 20 CDetermine the optical rotation in accordance with ISO 592.6.4 Chromatographic profileDetermine the chromatographic profile in accordance with ISO 11024.7 Packaging, labelling, marking and storageThese items shall be in accordance with ISO/TR 210 and ISO/TR 211. ISO 2014 All rights reserved 3BS
29、 ISO 16928:2014ISO 16928:2014(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of ginger (Zingiber officinale Roscoe)4 ISO 2014 All rights reservedBS ISO 16928:2014ISO 16928:2014(E)KeyPeak identification Operating conditions1 -Pinene Column: ca
30、pillary, fused silica; length 60 m; internal diameter 0,25 mmStationary phase: polydimethy siloxane (DB-1)aFilm thickness: 0,25 mOven temperature: isothermal at 60 C for 2 min, then temperature programming from 60 C to 150 C at a rate of 3 C/min, temperature programming from 150 C to 180 C at a rate
31、 of 1 C/min, temperature programming from 180 C to 220 C at a rate of 5 C/min, isothermal at 220 C for 5 minInjector temperature: 250 CDetector temperature: 250 CDetector: flame ionization typeCarrier gas: nitrogenVolume injected: 0,2 lCarrier gas flow rate: 1 ml/minSplit ratio: 1/100t time2 Camphen
32、e3 Methyl heptenone4 -Myrcene5+6+7 Limonene + 1,8-Cineole + -phellandrene8 Linalool9 Camphor10 Borneol11 Neral12 Geraniol13 Geranial14 Geranyl acetate15 -Elemene16 ar-Curcumene17 -Zingiberene18 -Farnesene19 -Bisabolene20 -SesquiphellandreneaExample of a suitable product available commercially. This
33、information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.1 Typical chromatogram taken on an apolar column for essential oil of ginger, produced in China ISO 2014 All rights reserved 5BS ISO 16928:2014ISO 16928:2014(E)Ke
34、yPeak identification Operating conditions1 -Pinene Column: capillary, fused silica; length 60 m; internal diameter 0,25 mmStationary phase: polyethylene glycol (Innowax 20 M)aFilm thickness: 0,25 mOven temperature: isothermal at 60 C for 2 min, then temperature programming from 60 C to 120 C at a ra
35、te of 3 C/min, temperature programming from 120 C to 145 C at a rate of 1 C/min, temperature programming from 145 C to 220 C at a rate of 5 C/min, isothermal at 220 C for 5 minInjector temperature: 250 CDetector temperature: 250 CDetector: flame ionization typeCarrier gas: heliumVolume injected: 0,2
36、 lCarrier gas flow rate: 1 ml/minSplit ratio: 1/100t time2 Camphene3 -Myrcene4 Limonene5+6 1,8-Cineole + -phellandrene7 Methyl heptenone8 Camphor9 Linalool10 -Elemene11 Neral12 Borneol13 -Zingiberene14 -Bisabolene15 Geranial16 -Farnesene17 Geranyl acetate18 -Sesquiphellandrene19 ar-Curcumene20 Geran
37、iolaExample of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.2 Typical chromatogram taken on a polar column for essential oil of ginger, produced in China6 ISO
38、2014 All rights reservedBS ISO 16928:2014ISO 16928:2014(E)KeyPeak identification Operating conditions1 -Pinene Column: capillary, fused silica; length 60 mStationary phase: methyl polysiloxane (DB-5)aOven temperature: temperature programming from 70 C to 270 C at a rate of 2 C/minInjector temperatur
39、e: 280 CDetector temperature: 280 CDetector: flame ionization typeCarrier gas: heliumt time2 Camphene3 -Phellandrene4 1,8-Cineole5 ar-Curcumene6 Germacrene D7 -Zingiberene8 -Farnesene9 -Bisabolene10 -SesquiphellandreneaExample of a suitable product available commercially. This information is given f
40、or the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.3 Typical chromatogram taken on an apolar column for essential oil of ginger, produced in India ISO 2014 All rights reserved 7BS ISO 16928:2014ISO 16928:2014(E)KeyPeak identification O
41、perating conditions1 -Pinene Column: capillary, fused silica; length 60 mStationary phase: polyethylene glycol (Carbowax)aOven temperature: isothermal at 60 C for 2 min, then temperature programming from 60 C to 120 C at a rate of 2,5 C/min, temperature programming from 120 C to 130 C at a rate of 0
42、,1 C/min, temperature programming from 130 C to 220 C at a rate of 8 C/min, isothermal at 220 C for 20 minInjector temperature: 240 CDetector temperature: 240 CDetector: flame ionization typeCarrier gas: heliumt time2 Camphene3+4 -Phellandrene + 1,8-Cineole5 Germacrene D6 -Zingiberene7 -Bisabolene8
43、-Farnesene9 -Sesquiphellandrene10 ar-CurcumeneaExample of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.4 Typical chromatogram taken on a polar column for essen
44、tial oil of ginger, produced in India8 ISO 2014 All rights reservedBS ISO 16928:2014ISO 16928:2014(E)KeyPeak identification Operating conditions1 -Pinene Column: capillary, fused silica; length 50 m; internal diameter 0,22 mmStationary phase: dimethyl polysiloxane (BP 1)aFilm thickness: 0,25 mOven t
45、emperature: isothermal at 65 C for 5 min, then temperature programming from 65 C to 185 C at a rate of 2C/min, temperature programming from 185 C to 230 C at a rate of 3 C/min, isothermal at 230 C for 35 minInjector temperature: 230 CDetector temperature: 250 CDetector: flame ionization typeCarrier
46、gas: hydrogenVolume injected: 0,2 lPre-column pressure: 152 KPaSplit ratio: 1/100t time2 Camphene3 Neral4 Geraniol5 Geranial6 -Elemene7 ar-Curcumene8 -Zingiberene9 -Bisabolene + -Farnesene10 -Sesquiphellandrene11 LinaloolaExample of a suitable product available commercially. This information is give
47、n for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.5 Typical chromatogram taken on an apolar column for essential oil of ginger, produced in West Africa ISO 2014 All rights reserved 9BS ISO 16928:2014ISO 16928:2014(E)KeyPeak identif
48、ication Operating conditions1 -Pinene Column: capillary, fused silica; length 50 m; internal diameter 0,22 mmStationary phase: polyethylene glycol (BP 20)aFilm thickness: 0,25 mOven temperature: isothermal at 65 C for 5 min, then temperature programming from 65 C to 185 C at a rate of 2C/min, temper
49、ature programming from 185 C to 230 C at a rate of 3 C/min, isothermal at 230 C for 35 minInjector temperature: 230 CDetector temperature: 250 CDetector: flame ionization typeCarrier gas: hydrogenVolume injected: 0,2 lPre-column pressure: 152 KPaSplit ratio: 1/100t time2 Camphene3 Linalool4 -Elemene5 neral6 -Zingiberene7 -Bisabolene + Geranial8 -Farnesene9 -Sesquiphel
