1、 g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58ICS 71.100.60Oil of magnolia flower, China type (Michelia alba DC)BRITISH STANDARDBS ISO 17382:2007
2、BS ISO 17382:2007This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2007 BSI 2007ISBN 978 0 580 55569 5Amendments issued since publicationAmd. No. Date CommentsCompliance with a British Standard cannot confer immunity from legal obligati
3、ons.National forewordThis British Standard is the UK implementation of ISO 17382:2007.The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication
4、does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Reference numberISO 17382:2007(E)INTERNATIONAL STANDARD ISO17382First edition2007-07-01Oil of magnolia flower, China type (Michelia alba DC) Huile essentielle de fleurs de magnol
5、ia, type Chine (Michelia alba DC)BS ISO 17382:2007ii iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees
6、. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Intern
7、ational Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Sta
8、ndards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subj
9、ect of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 17382 was prepared by Technical Committee ISO/TC 54, Essential oils. BS ISO 17382:2007blank1Oil of magnolia flower, China type (Michelia alba DC) 1 Scope This International Standard specifies c
10、ertain characteristics of the oil of Magnolia flower, China type (Michelia alba DC), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applie
11、s. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampl
12、ing ISO 279, Essential oils Determination of relative density at 20 C (Reference method) ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 709, Essential oils Determination of ester value ISO 875, Essential oils Evaluation of misc
13、ibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 1271, Essential oils Determination of carbonyl value Free hydroxylamine method ISO 4715, Essential oils Quantitative evaluation of residue on evaporation ISO 11024-1, Essential oils General guidance on chromatographic profil
14、es Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the follow
15、ing terms and definitions apply. 3.1 oil of Magnolia flower, China type essential oil obtained by steam distillation of the flower Michelia alba DC, of the Magnoliace family, growing wild or cultivated in the south of China NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1
16、 Appearance Clear, transparent, mobile liquid. 4.2 Colour Pale yellow to amber. 4.3 Odour Flowery, characteristic of Magnolia flower. 4.4 Relative density at 20 C, 2020d Minimum: 0,880 Maximum: 0,910 BS ISO 17382:20072 4.5 Refractive index at 20 C Minimum: 1,465 Maximum: 1,490 4.6 Optical rotation a
17、t 20 C Range from 15 to 5 4.7 Miscibility in ethanol, 95 % (volume fraction), at 20 C It shall not be necessary to use more than 1 volume of ethanol 95 % (volume fraction) to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 8,5 4.9 Ester value Minimum: 20 Maximum: 50 4
18、.10 Residue on evaporation Maximum: 15 % 4.11 Carbonyl value Maximum: 60 4.12 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The pro
19、portions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. 4.13 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 150 ml. NOTE This volu
20、me allows each of the tests specified in this International Standard to be carried out at least once. Table 1 Chromatographic profile Component Minimum % Maximum% Methyl 2-methylbutanoate 0,7 6,3 1,8-Cineole 0,3 0,8 trans-Ocimene 1,1 3,4 cis-Ocimene 0,9 2,4 -Copne 0,1 0,8 Linalool 50,0 72,0 -Elemene
21、 2,1 10,0 trans-Caryophyllene 1,2 7,0 -Cadinene 0,3 2,5 Nerol traces 0,3 trans-Methyl eugenol 1,2 4,4 Nerolidol 0,2 0,6 trans-Methyl isoeugenol 0,3 1,8 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 6 Test methods 6.1 Relative densi
22、ty at 20 C, 2020d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 95 % (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Ester value See ISO 709. BS ISO 17382:200736.7 Residue on evaporation See ISO 4
23、715. Test portion: 2,0 g Evaporation time: 3 h 6.8 Carbonyl value See ISO 1271. Saponification time: 1 h. 6.9 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 17382:20074 Annex A (informative) Typical chromatog
24、rams of the analysis by gas chromatography of the essential oil of Magnolia flower, China type (Michelia alba DC) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Methyl 2-methylbutanoate 1,8-Cineole cis-Ocimene trans-Ocimene -Copne -Cubebene Linalool -Elemene tr
25、ans-Caryophyllene -Terpineol -Selinene -Cadinene Nerol Geraniol trans-Methyl eugenol Nerolidol trans-Methyl isoeugenol 9,12-Octadecadienoic acid (Z,Z), methyl ester Column: fused capillary silica, 30 m length, 0,32 mm internal diameter Stationary phase: poly(dimethyl siloxane) (OV-1) Film thickness:
26、 1,5 m Oven temperature: isothermal at 80 C for 2 min, then programming temperature from 80 C to 100 C at a rate of 2 C/min, isothermal at 100 C for 8 min, then programming temperature from 100 C to 200 C at a rate of 5 C/min and isothermal at 200 C for 10 min, then programming temperature from 200
27、C to 220 C at a rate of 10 C/min and isothermal at 220 C for 8 min Injector temperature: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 ml/min Split ratio: 1/80 Figure A.1 Typical chromatogram taken on an apol
28、ar column BS ISO 17382:20075Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Methyl 2-methylbutanoate 1,8-Cineole cis-Ocimene trans-Ocimene -Copne -Cubebene Linalool -Elemene trans-Caryophyllene -Terpineol -Selinene -Cadinene Nerol Geraniol trans-Methyl eugenol N
29、erolidol trans-Methyl isoeugenol 9,12-Octadecadienoic acid (Z,Z), methyl ester Column: fused capillary silica, 30 m length, 0,32 mm internal diameter Stationary phase: polyethylene glycol 20 000 (PEG-20M) Film thickness: 0,5 m Oven temperature: isothermal at 80 C for 2 min, then programming temperat
30、ure from 80 C to 100 C at a rate of 2 C/min, isothermal at 100 C for 8 min, then programming temperature from 100 C to 200 C at a rate of 5 C/min and isothermal at 200 C for 10 min, then programming temperature from 200 C to 220 C at a rate of 10 C/min and isothermal at 220 C for 8 min Injector temp
31、erature: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 ml/min Split ratio: 1/80 Figure A.2 Typical chromatogram taken on a polar column BS ISO 17382:20076 Annex B (informative) Flashpoint B.1 General informat
32、ion For safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it
33、was difficult to recommend a single apparatus for standardization purposes, given that there is wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high-priced essential oils; as there are several different typ
34、es of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties.
35、The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oil of Magnolia flower, China type The mean value is +70 C. NOTE Obtained with “closed cup” equipment. BS ISO 17382:20077Bibliography 1 ISO/TR 11018:1997,
36、Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 17382:2007BS ISO BSI389 Chiswick High RoadLondonW4 4AL17382:2007BSI British Standards InstitutionBSI is the independent national body responsible for preparing British Standards.
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