1、BS ISO 18071:2016Fine ceramics (advancedceramics, advanced technicalceramics) Determinationof antiviral activity ofsemiconducting photocatalyticmaterials under indoor lightingenvironment Test methodusing bacteriophage Q-betaBSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013
2、15:06BS ISO 18071:2016 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 18071:2016.The UK participation in its preparation was entrusted to TechnicalCommittee RPI/13, Advanced technical ceramics.A list of organizations represented on this committee can beobtaine
3、d on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2016.Published by BSI Standards Limited 2016ISBN 978 0 580 85735 5ICS 81.060.30Compliance with a B
4、ritish Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 July 2016.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 18071:2016 ISO 2016Fine ceramics (advanc
5、ed ceramics, advanced technical ceramics) Determination of antiviral activity of semiconducting photocatalytic materials under indoor lighting environment Test method using bacteriophage Q-betaCramiques techniques Dtermination de lactivit antivirale des matriaux photocatalytiques semi-conducteurs da
6、ns un environnement dclairage intrieur Mthode dessai utilisant un bactriophage Q-btaINTERNATIONAL STANDARDISO18071First edition2016-07-15Reference numberISO 18071:2016(E)BS ISO 18071:2016ISO 18071:2016(E)ii ISO 2016 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2016, Published in SwitzerlandAl
7、l rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested f
8、rom either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 18071:2016ISO 18071:2016(E)Foreword vIntroduction vi1 S
9、cope . 12 Normative references 13 Terms and definitions . 14 Symbols 25 Principle 36 Materials . 36.1 Strains and preparation for tests 36.1.1 Strains . 36.1.2 Bacteria preparation. 46.1.3 Bacteriophage preparation . 46.2 Media . 56.2.1 General 56.2.2 1/500 Nutrient broth (1/500 NB) 56.2.3 Calcium s
10、olution . 56.2.4 LB broth with calcium . 56.2.5 Agar powder 56.2.6 LB agar 56.2.7 Bottom agar plate (LB agar plate with calcium) . 66.2.8 Top agar . 66.2.9 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP) 66.2.10 Peptone saline solution 67 Apparatus and equipment 67.1 Test equipm
11、ent . 67.2 Cover film . 77.3 Moisture preservation glass 77.4 Glass tube or glass rod 77.5 Paper filter 77.6 Light source . 77.7 UV sharp cut-off filter 87.7.1 Condition A (under 400 nm cut-off condition) 87.7.2 Condition B (under 380 nm cut-off condition) 87.8 Illuminance meter 87.9 Centrifuge 87.1
12、0 Sterilized syringe filter unit . 88 Test piece 89 Procedure. 89.1 General . 89.2 Procedure for preparation of bacteria suspension 99.3 Procedure of preparation of test bacteriophage solution 99.4 Procedure of test for indoor-light-active photocatalytic antiviral activity .109.5 Indoor lighting con
13、dition 109.6 Measurement of titre of bacteriophage 1110 Calculation .1210.1 General 1210.2 Test requirement fulfilment validation . 1210.3 Indoor-light-active photocatalyst antiviral activity value calculation 1310.4 Antiviral activity value calculation without indoor-light-active photocatalyst 1311
14、 Test report 14 ISO 2016 All rights reserved iiiContents PageBS ISO 18071:2016ISO 18071:2016(E)Bibliography .15iv ISO 2016 All rights reservedBS ISO 18071:2016ISO 18071:2016(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
15、 member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, gover
16、nmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintena
17、nce are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attentio
18、n is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction an
19、d/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity a
20、ssessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.The committee responsible for this document is ISO/TC 206, Fine ceramics. ISO 2016 All rights reserved
21、 vBS ISO 18071:2016ISO 18071:2016(E)IntroductionThis International Standard applies to testing the antiviral activity of indoor-light-active photocatalytic ceramics and other materials produced by either coating or mixing of a light-active photocatalyst. The International Standard for testing the an
22、tibacterial activity of photocatalytic materials has been published as ISO 27447 and the International Standard for testing the antibacterial activity of indoor-light-active photocatalytic materials has been published as ISO 17094. The International Standard for determination of antiviral activity o
23、f semiconducting photocatalytic materials has also been published as ISO 18061.The test method for cloths or textiles is not included in this International Standard because of lack of indoor-light-active photocatalytic cloths or textiles. When the indoor-light-active photocatalytic cloths or textile
24、s with antiviral activity using indoor-light-active photocatalytic activity have been developed, a test method for indoor-light-active photocatalytic cloths or textiles will be proposed with the glass adhesion method in ISO 27447.vi ISO 2016 All rights reservedBS ISO 18071:2016Fine ceramics (advance
25、d ceramics, advanced technical ceramics) Determination of antiviral activity of semiconducting photocatalytic materials under indoor lighting environment Test method using bacteriophage Q-betaWARNING Only personnel trained in microbiological techniques should carry out tests.1 ScopeThis Internationa
26、l Standard specifies the determination of the antiviral activity of materials that contain indoor-light-active photocatalytic materials or have indoor-light-active photocatalytic films on the surface by a test method that measures the infectivity titre of bacteriophage Q-beta after illumination with
27、 indoor light.NOTE In the test method, the surrogate microbe is bacteriophage Q-beta, intended as a model for influenza viruses.This International Standard is intended for use with different kinds of indoor-light-active photocatalytic materials used in construction materials, in flat sheet, board or
28、 plate shape that are the basic forms of materials for various applications. It does not include powder, granular or porous indoor-light-active photocatalytic materials.This International Standard is applicable to indoor-light-active photocatalytic materials produced for an antiviral applications. O
29、ther types of performance of indoor-light-active photocatalytic materials, i.e. antibacterial activity, antifungal activity, decomposition of water contaminants, self-cleaning, antifogging and air purification, are not determined by this method.2 Normative referencesThe following documents, in whole
30、 or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 14605, Fine ceramics (advanced cerami
31、cs, advanced technical ceramics) Light source for testing semiconducting photocatalytic materials used under indoor lighting environmentISO 27447, Fine ceramics (advanced ceramics, advanced technical ceramics) Test method for antibacterial activity of semiconducting photocatalytic materialsISO 80000
32、-1, Quantities and units Part 1: General3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1photocatalystsubstance that carries out many functions based on oxidization and reduction reactions under photoirradiation, including decomposition and remov
33、al of air and water contaminants, deodorization, and antiviral, antibacterial, antifungal, self-cleaning and antifogging actionsINTERNATIONAL STANDARD ISO 18071:2016(E) ISO 2016 All rights reserved 1BS ISO 18071:2016ISO 18071:2016(E)3.2indoor-light-active photocatalystsubstance that reacts with arti
34、ficial light source for general lighting service (i.e. indoor lighting environment)3.3indoor lighting environmentenvironment with artificial light source for general lighting serviceNote 1 to entry: Does not include sunlight.3.4indoor-light-active photocatalytic materialsmaterials in which or on whi
35、ch the indoor-light-active photocatalyst is added by coating, impregnation, mixing, etc.3.5antiviralcondition decreasing the infectivity of viruses on the surface of materials3.6bacteriophagetype of virus which infects bacteriaNote 1 to entry: The bacteriophage used in this International Standard is
36、 Q-beta that is one of F-specific RNA phages. The bacteriophage Q-beta is not pathogenic to humans and animals, but serves to simulate Influenza viruses that are pathogenic to humans.3.7plaquevisible, clear area which is theoretically the result of infection and lysis of host cells by a single viabl
37、e bacteriophage3.8indoor-light-active photocatalyst antiviral activity valuedifference between the logarithms of the total number of bacteriophage plaques on photocatalytic treated materials after indoor light illumination and on non-treated materials after indoor light illuminationNote 1 to entry:
38、This value includes the decrease of number of bacteriophage plaques without indoor light illumination.3.9indoor-light-active photocatalyst antiviral activity value for indoor light illuminationdifference between the logarithms of the total number of bacteriophage plaques on photocatalytic treated ma
39、terials after indoor light illumination and on photocatalytic treated materials kept in a dark place4 SymbolsA average of titre of bacteriophage on non-treated specimens, just after inoculationBDaverage of titre of bacteriophage on non-treated specimens, after being kept in a dark placeBF-Laverage o
40、f titre of bacteriophage on non-treated specimens, after indoor light illumination of intensity L under condition FCDaverage of titre of bacteriophage on indoor-light-active photocatalytic treated specimens, after being kept in a dark place2 ISO 2016 All rights reservedBS ISO 18071:2016ISO 18071:201
41、6(E)CF-Laverage of titre of bacteriophage on indoor-light-active photocatalytic treated specimens, after indoor light illumination of intensity L under condition FDFdilution factorF type of UV cut-off condition (condition A or condition B)L illuminance of indoor lightLogmax maximum logarithmic value
42、 of titre of bacteriophageLogmean average logarithmic value of titre of bacteriophage for three non-treated specimensLogmin minimum logarithmic value of titre of bacteriophageN titre of bacteriophage (plaque forming unit)VDantiviral activity value without indoor-light-active photocatalyst, after bei
43、ng kept in a dark place on a testing materialVF-Lindoor-light-active photocatalyst antiviral activity value, after indoor light illumination at a constant intensity (F-L) on an indoor-light-active photocatalytic materialV indoor-light-active photocatalyst antiviral activity value with indoor light i
44、lluminationZ average number of plaques in two Petri dishes5 PrincipleThe test method is suitable for use in development, comparison, quality assurance, characterization, reliability and design data generation of indoor-light-active photocatalytic materials. The method is used to obtain the antiviral
45、 activity of indoor-light-active photocatalytic materials by the contact of a specimen with bacteriophage under indoor lighting condition. The method is suitable for use with flat sheet, board or plate-shaped materials.The specimen of indoor-light-active photocatalytic treated material is inoculated
46、 with bacteriophage suspension and exposed to light for a specified period. Following exposure, the test suspension is removed and measured by the plaque forming method with Escherichia coli which is sensitive to bacteriophage Q-beta. The results obtained are compared with those obtained from inocul
47、ated specimens of non-photocatalytic treated material exposed to light under identical conditions to the treated material and to those obtained from inoculated specimens of both photocatalytic treated and non-treated material kept in the dark for the same period of time.NOTE This International Stand
48、ard is adapted from the common methodological concept for ISO 18061. Namely, the same apparatus without light source (see 7.6), UV sharp cut-off filter (see 7.7), and test piece size, similar procedure and calculation are adapted between this International Standard and ISO 18061. Therefore, ISO 1806
49、1 is recommended to be used as reference during actual test of this International Standard.6 Materials6.1 Strains and preparation for tests6.1.1 StrainsThe strains to be used in the test shall be the same as or equivalent to those described in Table 1 and supplied by an entity that is registered under the World Federation for Culture Collections or the Japan Society for Culture Collections. Aseptic manipulations using microorganisms can be performed in an appropriate safety cabinet. ISO 2016 All rights reserved 3BS ISO
