1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 19012-1:2013Microscopes Designation ofmicroscope objectivesPart 1: Flatness of field/PlanBS ISO 19012-1:2013 BRITISH STANDARDNational forewordThis British Standard is the
2、UK implementation of ISO 19012-1:2013.It supersedes BS ISO 19012-1:2011 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee CPW/172, Optics and Photonics.A list of organizations represented on this committee can beobtained on request to its secretary.This p
3、ublication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 79527 5ICS 37.020Compliance with a British Standard cannot confer immunity
4、fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 May 2013.Amendments issued since publicationDate Text affectedBS ISO 19012-1:2013 ISO 2013Microscopes Designation of microscope objectives Part 1: Flatness of field/PlanM
5、icroscopes Dsignation des objectifs de microscope Partie 1: Planit du champ/PlanINTERNATIONAL STANDARDISO19012-1Third edition2013-05-15Reference numberISO 19012-1:2013(E)BS ISO 19012-1:2013ISO 19012-1:2013(E)ii ISO 2013 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2013All rights reserved. Unl
6、ess otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the
7、address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 19012-1:2013ISO 19012-1:2013(E) ISO 2013 All rights reserved iiiConte
8、nts PageForeword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 24.1 Indication . 24.2 Definition of plan objectives 24.3 Determination of plan field number . 2Annex A (informative) Depth of field in object space calculated by Bereks formula . 4Bibliography 7BS ISO 19
9、012-1:2013ISO 19012-1:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body intereste
10、d in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Com
11、mission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents
12、should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directivesAttention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for ident
13、ifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received. www.iso.org/patentsAny trade name used in this document is information given for the convenience of
14、 users and does not constitute an endorsement.The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes.This third edition cancels and replaces the second edition (ISO 19012-1:2011) by the addition of “Petzval curvature”.ISO 19012
15、consists of the following parts, under the general title Microscopes Designation of microscope objectives: Part 1: Flatness of field/Plan Part 2: Chromatic correctionThe following parts are under preparation: Part 3: Spectral transmittanceiv ISO 2013 All rights reservedBS ISO 19012-1:2013INTERNATION
16、AL STANDARD ISO 19012-1:2013(E)Microscopes Designation of microscope objectives Part 1: Flatness of field/Plan1 ScopeThis part of ISO 19012 specifies the use of the marking “Plan” on microscope objectives, and defines the diameter of the sharp region of the primary image of a flat object surface. Th
17、is part of ISO 19012 applies to visual observation using the combination of objective lens, tube lens and eyepiece, as specified by the manufacturer.This marking is consistent with ISO 8578.NOTE The flatness of the image field does not imply any degree of correction for other aberrations (ISO 10934-
18、1).2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any ame
19、ndments) applies.ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light microscopy3 Terms and definitionsFor the purposes of this document, the terms and definitions given in ISO 10934-1 and the following apply.3.1tangential structured objectobject containing short lines
20、 perpendicular to the radii of the object field3.2tangential image surfacesurface on which all tangential structured objects are focused and sharply imaged in the primary image space subject to no aberrations other than astigmatism and Petzval curvature3.3sagittal structured objectobject containing
21、short lines parallel to the radii of the object field3.4sagittal image surfacesurface on which all sagittal structured objects are focused and sharply imaged in the primary image space subject to no aberrations other than astigmatism and Petzval curvature3.5astigmatic differencedimensional differenc
22、e along the optical axis in the tangential plane between the tangential and sagittal image surfaces ISO 2013 All rights reserved 1BS ISO 19012-1:2013ISO 19012-1:2013(E)3.6plan field numberPFNnumber which specifies the diameter, in millimetres, of the sharp region of the primary image of a flat objec
23、t surface3.7objective field numberOFNmaximum field of view number of the eyepiece for which the objective is designed to be used3.8plan field ratioPFRratio of the plan field number to the objective field number, defined as PFR = PFN/OFN3.9Petzval curvaturecurvature of the Petzval surface, which deno
24、tes the basic field curvature4 Requirements4.1 IndicationObjective lenses named Plan or with Plan as part of the name in the markings shall also indicate the objective field number on the body of the lens. If the words “flat field” are used in the name in the marking, then the lenses shall also be m
25、arked “Plan” with an indication of the OFN on the body of the lens. The indication of objective field number does not apply to objective lenses sold before the year 2014.Objective field numbers shall be expressed as follows:18, 19, 20, 21, 22, 23, 24, 25, 26, 26.5, 27, 28, 29, 30 and so onEXAMPLE In
26、 the case of the objective field number 25:OFN254.2 Definition of plan objectivesThe plan field ratio of a plan objective lens shall be at least 0,85.4.3 Determination of plan field numberLet tand sbe the respective distances of the tangential and sagittal image surfaces from the image plane, along
27、the optical axis in a tangential plane. Using tand s, the average image surface distance, , is defined as shown in Formula (1): = (t+ s)/2 (1)The plan field number shall be specified by the maximum field of view of the primary image which satisfies the following conditions: the absolute values of bo
28、th and astigmatic difference (t s) are 2 ISO 2013 All rights reservedBS ISO 19012-1:2013ISO 19012-1:2013(E)less than or equal to the value calculated by Bereks formula see Formula (2), and the magnification of the eyepiece is 10 .=+MNANAMTOT VIS2022500002(2)where is the depth of focus in image space
29、, in micrometres; is a physiological constant which describes the resolution of the human eye, taken to be the angle 5 is the arc of this angle (0,001 4);MTOT VISis the total visual magnification of the microscope;NA is the numerical aperture of objective; is the wavelength of the e-line, in microme
30、tres;M0is the magnification in the primary image plane.The depth of field calculated by Bereks formula is expressed in Annex A. ISO 2013 All rights reserved 3BS ISO 19012-1:2013ISO 19012-1:2013(E)Annex A (informative) Depth of field in object space calculated by Bereks formulaBereks formula is as fo
31、llows:ob= +nMNANATOT VIS22500002 = 0,001 4, = 0,55 m 4 ISO 2013 All rights reservedBS ISO 19012-1:2013ISO 19012-1:2013(E)Magnification of objective lens 4 4 4 4 5 5 5NA of objective lens 0,10 0,13 0,16 0,20 0,12 0,15 0,16Magnification of eyepiece 10 10 10 10 10 10 10Depth of field at specimen:ob(m)1
32、14,825 83,476 65,361 50,581 77,309 58,811 54,424Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1 1 1Magnification of objective lens 10 10 10 10 10 10NA of objective lens 0,22 0,25 0,30 0,32 0,40 0,45Magnification of eyepiece 10 10 10 10 10 10Depth of field at specimen:ob(m)21,555 1
33、8,372 14,703 13,606 10,458 9,127Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1 1Magnification of objective lens 20 20 20 20 20 20NA of objective lens 0,40 0,45 0,50 0,60 0,70 0,75Magnification of eyepiece 10 10 10 10 10 10Depth of field at specimen:ob(m)6,083 5,238 4,593 3,676 3,
34、058 2,819Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1 1Magnification of objective lens 40 40 40 40 40 40 40 40 40 40NA of objective lens 0,55 0,60 0,65 0,70 0,75 0,85 0,95 1,00 1,25 1,30Magnification of eyepiece 10 10 10 10 10 10 10 10 10 10Depth of field at specimen:ob(m)2,494
35、 2,217 1,993 1,808 1,652 1,408 1,224 1,740 1,325 1,265Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1 1 1 1,515 1,515 1,515Magnification of objective lens 60 60 60 60 60 60 60NA of objective lens 0,70 0,85 0,90 0,95 1,25 1,30 1,40Magnification of eyepiece 10 10 10 10 10 10 10Depth
36、 of field at specimen:ob(m)1,391 1,064 0,985 0,917 0,972 0,925 0,842Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1,515 1,515 1,515 ISO 2013 All rights reserved 5BS ISO 19012-1:2013ISO 19012-1:2013(E)Magnification of objective lens 63 63 63 63 63 63 63NA of objective lens 0,70 0,7
37、5 0,80 0,95 1,25 1,32 1,40Magnification of eyepiece 10 10 10 10 10 10 10Depth of field at specimen:ob(m)1,351 1,227 1,121 0,888 0,938 0,875 0,812Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1 1 1,515 1,515 1,515Magnification of objective lens 100 100 100 100 100 100NA of objective le
38、ns 0,90 0,95 1,25 1,30 1,35 1,40Magnification of eyepiece 10 10 10 10 10 10Depth of field at specimen:ob(m)0,726 0,671 0,689 0,653 0,620 0,590Refractive index: n(dry: n = 1, oil immersion: n = 1,515)1 1 1,515 1,515 1,515 1,5156 ISO 2013 All rights reservedBS ISO 19012-1:2013ISO 19012-1:2013(E)Biblio
39、graphy1 ISO 8578, Microscopes Marking of objectives and eyepieces2 Berek M Grundlagen der Tiefenwahrnehmung im Mikroskop. Marburger Sitzungsberichte. 1927, 62 pp. 189223 ISO 2013 All rights reserved 7BS ISO 19012-1:2013ISO 19012-1:2013(E) ISO 2013 All rights reservedICS 37.020Price based on 7 pagesT
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