1、BS ISO 20311:2017Traditional Chinese medicine Salvia miltiorrhiza seeds and seedlingsBSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS ISO 20311:2017 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 20311:2017. The UK participat
2、ion in its preparation was entrusted to TechnicalCommittee CH/100/1, Traditional Chinese Medicine.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are respons
3、ible for its correctapplication. The British Standards Institution 2017. Published by BSI Standards Limited 2017ISBN 978 0 580 93284 7ICS 65.020.20Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards P
4、olicy and Strategy Committee on 31 March 2017.Amendments/corrigenda issued since publicationDate Text affectedBS ISO 20311:2017 ISO 2017Traditional Chinese medicine Salvia miltiorrhiza seeds and seedlingsMdecine traditionnelle chinoise Graines et plants de Salvia miltiorrhizaINTERNATIONAL STANDARDIS
5、O20311First edition2017-03Reference numberISO 20311:2017(E)BS ISO 20311:2017ISO 20311:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherw
6、ise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh.
7、 de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 20311:2017ISO 20311:2017(E)Foreword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Descriptions 35 Requirements 55.1 General characteristics . 55.2
8、 Salvia miltiorrhiza seeds . 55.3 Seedlings. 66 Sampling 66.1 Seed sampling 66.2 Seedling sampling 67 Test method . 77.1 Moisture 77.2 Maturity 77.3 Purity . 77.4 Seed viability. 77.5 1 000-seed weight 77.6 Germination percentage . 77.7 Fungus testing . 77.7.1 Preparation of seed 77.7.2 Preparation
9、of seedling 87.7.3 Test of Alternaria spp. . 87.7.4 Test of Fusarium spp. 87.8 Nematode testing 87.9 Seedling diameter . 87.10 Seedling length 88 Test report . 89 Packaging, storage and transportation . 810 Marking 9Bibliography .10 ISO 2017 All rights reserved iiiContents PageBS ISO 20311:2017ISO 2
10、0311:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject fo
11、r which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on
12、all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted.
13、 This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying
14、any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience
15、of users and does not constitute an endorsement. For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical
16、Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by Technical Committee ISO/TC 249, Traditional Chinese medicine.iv ISO 2017 All rights reservedBS ISO 20311:2017INTERNATIONAL STANDARD ISO 20311:2017(E)Traditional Chinese medicine Salvia mil
17、tiorrhiza seeds and seedlings1 ScopeThis document specifies the minimum requirements and test method for Salvia miltiorrhiza seeds and seedlings. It is suitable for use in quality assurance during the production and management of Salvia miltiorrhiza seeds and seedlings.2 Normative referencesThe foll
18、owing documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.Intern
19、ational Seed Testing Association (ISTA), International Rules for Seed TestingInternational Seed Testing Association (ISTA), Working Sheets on Tetrazolium testing3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological dat
20、abases for use in standardization at the following addresses: IEC Electropedia: available at http:/ www .electropedia. org/ ISO Online browsing platform: available at http:/ www. iso. org/ obp3.1seed lotspecified quantity of seed (3.7) that is physically and uniquely identifiableSOURCE: ISTA, Intern
21、ational Rules for Seed Testing, 2.2.13.2primary sampleportion taken from the seed lot (3.1) during one single sample actionSOURCE: ISTA, International Rules for Seed Testing, 2.2.23.3composite samplesample formed by combining and mixing all the primary samples (3.2) taken from the seed lot (3.1)SOUR
22、CE: ISTA, International Rules for Seed Testing, 2.2.3, modified3.4subsampleportion of a primary sample (3.2) obtained by reducing a sampleSOURCE: ISTA, International Rules for Seed Testing, 2.2.4 ISO 2017 All rights reserved 1BS ISO 20311:2017ISO 20311:2017(E)3.5submitted samplesample that is to be
23、submitted to the testing laboratory and may comprise either the whole of the composite sample (3.3) or a subsample (3.4)Note 1 to entry: The submitted sample may be divided into subsamples packed in different material meeting conditions for specific tests (e.g. moisture or health).SOURCE: ISTA, Inte
24、rnational Rules for Seed Testing, 2.2.53.6working samplewhole of the submitted sample (3.5) or a subsample (3.4), to which one of the quality tests described in the ISTA Rules is appliedNote 1 to entry: The working sample is at least the weight prescribed by the ISTA Rules for the particular test.SO
25、URCE: ISTA, International Rules for Seed Testing, 2.2.7, modified3.7seedmature ovule produced by Salvia miltiorrhiza BungeNote 1 to entry: It consists of three basic parts: embryo, endosperm and the seed coat.3.8seedlingyoung plant of Salvia miltiorrhiza Bunge after cultivation for one year, consist
26、ing of bud, tap root (3.14), lateral root and fibrous root3.9purityweight percentage of pure seed (3.7) fraction over the total weight of the working sample (3.6)Note 1 to entry: The pure seed refers to the species stated by the applicant, or found to predominate in the test and includes all botanic
27、al varieties and cultivars of that species.Note 2 to entry: It is expressed in per cent (%).SOURCE: ISTA, International Rules for Seed Testing, definition 3.2.1, modified3.10viabilitypotential ability of a seed (3.7) to germinate or the capability of an embryo to liveNote 1 to entry: It is the perce
28、ntage of stained seeds in the working sample (3.6), estimated using the Topographical Tetrazolium Test .3.111 000-seed weightaverage weight of every 1 000 pure seeds (3.7) of a working sample (3.6)3.12seed moisture contentloss of weight of seed (3.7) after drying over the weight of seed before dryin
29、g3.13germination percentagepercentage of germinated seed (3.7) over the working sample (3.6)3.14tap rootroot generated from the one year development of the radical after seed (3.7) germination2 ISO 2017 All rights reservedBS ISO 20311:2017ISO 20311:2017(E)3.15seedling lengthlargest distance from the
30、 lower part to the topNote 1 to entry: It is expressed in centimetres.4 DescriptionsSalvia miltiorrhiza seed is the dried seed of the plant Salvia miltiorrhiza Bunge and consists of three basic parts: the embryo, cytoledon and the pericarp (see Figure 1 C). The epidermal mucilage layer swells to a t
31、ransparent mucilage membrane surrounding the seed after soaking the seed with warm water (Figure 1 D). ISO 2017 All rights reserved 3BS ISO 20311:2017ISO 20311:2017(E)ABCD12345678Key1 seed length A facade of the seed2 seed thickness B back of the seed3 hilum C longitudinal section of the seed4 raphe
32、 D seed after soaking in water5 embryo6 pericarp7 cotyledon8 mucilage layerFigure 1 Structure of Salvia miltiorrhiza seedThe Salvia miltiorrhiza seedling is a one-year seedling, consisting of four parts: bud, tap root, lateral root and fibrous root, as shown in Figure 2.4 ISO 2017 All rights reserve
33、dBS ISO 20311:2017ISO 20311:2017(E)BA12345Key1 seedling length2 bud3 tap root4 lateral root5 fibrous rootA seedling with more lateral rootsB seedling with fewer lateral rootsFigure 2 Salvia miltiorrhiza seedling5 Requirements5.1 General characteristicsThe following requirements shall be met before s
34、eparating the bulk sample into test samples:a) Salvia miltiorrhiza seedlings shall be healthy and intact;b) the presence of living insects, mouldy seeds and external contaminants which are visible to thenaked eye shall not be permitted.5.2 Salvia miltiorrhiza seeds5.2.1 The colour of the seed shall
35、be black or brown. The seed shall have a hard texture, without any peculiar smell and mildew.5.2.2 The mass fraction of moisture shall not be greater than 10 %. ISO 2017 All rights reserved 5BS ISO 20311:2017ISO 20311:2017(E)5.2.3 Seed purity shall not be less than 70 %.5.2.4 Viability shall not be
36、less than 90 %.5.2.5 Maturity shall not be less than 90 %.5.2.6 The 1 000-seed weight, moisture content, germination percentage and seed purity shall comply with the requirements in Table 1.Table 1 Grading requirements of the Salvia miltiorrhiza seedsGrade1 000-seed weight gMoisture content %Germina
37、tion percentage %Purity %First 1,80 6,00 60,0 90,0Second 1,60 8,00 50,0 80,0Third 1,60 10 40,0 70,0NOTE The establishment of the above requirements is based on the seeds collected from different regions.5.3 Seedlings5.3.1 The tap root and lateral root of the Salvia miltiorrhiza seedling shall be int
38、act with no diseases or insects. The tap root shall be full and substantial.5.3.2 The bud of the Salvia miltiorrhiza seedling shall be intact with no diseases or insects.5.3.3 Nematode shall not be detected.6 Sampling6.1 Seed samplingSeed sampling shall be carried out in accordance with ISTA, Intern
39、ational Rules for Seed Testing, Chapter 2. The maximum weight of the lot and the minimum weight of the sample are specified in Table 2.Table 2 Maximum weight of seed lot and minimum weight of sampleMaximum weight of seed lot kgMinimum weight of sample gSubmitted sample Purity analysis2 000 250 50NOT
40、E The establishment of the above requirement is based on the seeds collected from different regions and handled with different treatments.6.2 Seedling samplingThe maximum sample lot of seedlings is 300 000 and the minimum samples should not be less than 100.6 ISO 2017 All rights reservedBS ISO 20311
41、:2017ISO 20311:2017(E)7 Test method7.1 MoistureDetermination of the mass fraction of moisture shall be in accordance with the high constant temperature method specified in ISTA, International Rules for Seed Testing.7.2 MaturityTake duplicate samples of dry seeds using the quartering method, each con
42、taining 100 to 150 seeds. The seed shall be cut into two parts along the raphe. The shape of its embryo is determined with a microscope. The seed is considered to be mature if its embryo is pyriform or saddle. Formula (1) is used to express the maturity of a seed lot:MA%()=mA100 (1)whereM is the mat
43、urity (%);Amis the number of mature seeds;A is the number of seeds in the working sample.If the difference between measured maturities of the duplicate sample is less than 5 %, then the maturity of the seed lot shall be the arithmetic mean of two measured values; otherwise, it shall be re-estimated.
44、7.3 PurityDetermination of the seed purity shall be in accordance with the purity analysis of ISTA, International Rules for Seed Testing.7.4 Seed viabilityDetermination of the seed viability shall be in accordance with ISTA, International Rules for Seed Testing and ISTA, Working Sheets on Tetrazoliu
45、m Testing.7.5 1 000-seed weightDetermination of the 1 000-seed weight shall be in accordance with Weight Determination of ISTA, International Rules for Seed Testing.7.6 Germination percentageGermination percentage shall be in accordance with the purity analysis of ISTA, International Rules for Seed
46、Testing.7.7 Fungus testing7.7.1 Preparation of seedAdd 100 seeds and 20 ml sterilized water into a sterilized flask and shake for 8 min. The suspension is then centrifuged at 4 000 rpm for 20 min. The residue is re-suspended with 2 ml of sterilized water. Spot 100 l of the final suspension on the bl
47、otter or filter paper on a plate for incubation. There shall be a total of five spots on the plate. ISO 2017 All rights reserved 7BS ISO 20311:2017ISO 20311:2017(E)7.7.2 Preparation of seedlingAdd 500 ml sterilized water into a flask. Take 10 to 20 seedlings. Immerge the seedling into the flask and
48、shake for 8 min one by one. The suspension is then centrifuged at 4 000 rpm for 20 min. The residue is re-suspended with 2 ml of sterilized water. Spot 100 micro litter of the final suspension on the blotter or filter paper in a plate for incubation. There shall be a total of five spots on the plate
49、.7.7.3 Test of Alternaria spp.Alternaria spp. testing shall be in accordance with ISTA, International Rules for Seed Testing, method 7-001a.7.7.4 Test of Fusarium spp.Fusarium spp. testing shall be in accordance with ISTA, International Rules for Seed Testing, method 7-009.7.8 Nematode testingTake 100 to 150 seedlings. Test nematode knots or lesions with the naked eye. Check the root knot or root lesion for nematode under microscope.7.9 Seedling diameterTake 100 to 150 seedlings and measure the top of
