1、BRITISH STANDARDBS ISO 21390:2005Oil of wintergreen, China Gaultheria yunnanensis Franch.) Rehd., redistilled ICS 71.100.60g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53
2、g44g42g43g55g3g47g36g58BS ISO 21390:2005This British Standard was published under the authority of the Standards Policy and Strategy Committee on 14 November 2005 BSI 14 November 2005ISBN 0 580 46830 5National forewordThis British Standard reproduces verbatim ISO 21390:2005 and implements it as the
3、UK national standard.The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary.Cross-referencesThe British Standards which impl
4、ement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online.This publication does not purpor
5、t to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee
6、any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK.Summary of pagesThis document comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a bl
7、ank page, pages 1 to 6, an inside back cover and a back cover.The BSI copyright notice displayed in this document indicates when the document was last issued.Amendments issued since publicationAmd. No. Date CommentsReference numberISO 21390:2005(E)INTERNATIONAL STANDARD ISO21390First edition2005-10-
8、15Oil of wintergreen, China Gaultheria yunnanensis (Franch.) Rehd., redistilled Huile essentielle de gaultherie, Chine Gaultheria yunnanensis (Franch.) Rehd., bidistille BS ISO 21390:2005ii iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national sta
9、ndards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International or
10、ganizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules give
11、n in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 7
12、5 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 21390 was prepared by Technical Committee ISO/TC 54, Ess
13、ential oils. BS ISO 21390:2005blank1Oil of wintergreen, China Gaultheria yunnanensis (Franch.) Rehd., redistilled 1 Scope This International Standard specifies certain characteristics of the oil of wintergreen, China Gaultheria yunnanensis (Franch.) Rehd., redistilled, in order to facilitate assessm
14、ent of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR
15、 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Dete
16、rmination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 709, Essential oils Determination of ester value ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 4715, Essential oils Quantitative evaluat
17、ion of residue on evaporation ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profile
18、s of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of wintergreen, China, redistilled essential oil obtained by steam distillation of the leaves, leaf stalks and young twigs of Gaultheria yunnanensis (Franch.)
19、Rehd., of the Ericaceae family, growing wild or cultivated in the south of China, followed by redistillation NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear, transparent, mobile liquid. 4.2 Colour Colourless to slight yellow. 4.3 Odour Strongly aromatic
20、with a sweet characteristic odour. 4.4 Relative density at 20 C, d2020Minimum: 1,160 Maximum: 1,195 4.5 Refractive index at 20 C Minimum: 1,523 0 Maximum: 1,543 0 BS ISO 21390:20052 4.6 Optical rotation at 20 C Between 1and +1. 4.7 Miscibility in ethanol, 80 % (volume fraction), at 20 C It shall not
21、 be necessary to use more than 4 volumes of ethanol, 80 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 1,5 4.9 Ester value Minimum: 338,0 Maximum: 380,0 4.10 Residue on evaporation Maximum: 0,1 % 4.11 Chromatographic profile Analysis of the es
22、sential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato-g
23、raphic profile of the essential oil. 4.12 Flashpoint Information on the flashpoint is given in Annex B. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene traces 0,20 -Pinene traces 0,05 1,8-Cineole traces 0,40 Linalool traces 0,20 Methyl salicylate 97,0 99,8 Ethyl salicylate trac
24、es 0,3 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 5 Sampling See ISO 212. Minimum volume of test sample: 150 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.
25、 6 Test methods 6.1 Relative density at 20 C, d2020See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 80 % (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Ester value See ISO 709. 6.7 Residue on evapor
26、ation See ISO 4715. Test portion: 2,0 g Evaporation time: 3 h 6.8 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 21390:20053Annex A (informative) Typical chromatograms of the analysis by gas chromatography of
27、 the essential oil of wintergreen, China Gaultheria yunnanensis (Franch.) Rehd., redistilled Peak identification Operating conditions 1 2 3 4 5 6 -Pinene -Pinene 1,8-Cineole Linalool Methyl salicylate Ethyl salicylate Column: fused silica capillary; length 30 m; internal diameter 0,32 mm Stationary
28、phase: polydimethylsiloxane (OV-1) Film thickness: 1,5 m Oven temperature: temperature programming from 100 C to 130 C at a rate of 2 C/min and isothermal at 130 C for 8 min, then temperature programming from 130 C to 200 C at a rate of 5 C/min and isothermal at 200 C for 10 min Injector temperature
29、: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 ml/min Split ratio: 80/1 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 21390:20054 Peak identification Operating conditions 1 2 3 4 5 6 -Pine
30、ne -Pinene 1,8-Cineole Linalool Methyl salicylate Ethyl salicylate Column: fused silica capillary; length 30 m; internal diameter 0,32 mm Stationary phase: polyethylene glycol 20 000 (PEG-20M) Film thickness: 0,5 m Oven temperature: temperature programming from 100 C to 130 C at a rate of 2 C/min an
31、d isothermal at 130 C for 8 min, then temperature programming from 130 C to 200 C at a rate of 5 C/min and isothermal at 200 C for 10 min Injector temperature: 250 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1,0 ml
32、/min Split ratio: 80/1 Figure A.2 Typical chromatogram taken on a polar column BS ISO 21390:20055Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of es
33、sential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that there is wide variation in the chemical composition of essenti
34、al oils; the volume of the sample needed for certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to gi
35、ve a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oil
36、 of wintergreen, China, redistilled The mean value is +96 C. NOTE Obtained with “closed cup” equipment. BS ISO 21390:20056 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 21390:2005blankBS ISO
37、 21390:2005BSI389 Chiswick High RoadLondonW4 4ALBSI British Standards InstitutionBSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter.RevisionsBritish Stand
38、ards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions.It is the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using
39、this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cover. Tel: +44 (0)20 8996 9000. Fax: +44 (0)20 8996 7400.BSI offers members an individual updating service called PLUS which ensures that subscribers autom
40、atically receive the latest editions of standards.Buying standardsOrders for all BSI, international and foreign standards publications should be addressed to Customer Services. Tel: +44 (0)20 8996 9001. Fax: +44 (0)20 8996 7001. Email: ordersbsi-. Standards are also available from the BSI website at
41、 http:/www.bsi-.In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been published as British Standards, unless otherwise requested.Information on standardsBSI provides a wide range of information on national, European and internati
42、onal standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also available which give details on all its products and services. Contact the Information Centre. Tel: +44 (0)20 8996 7111. Fax: +44 (0)20 8996 7048. Email: infobsi-.Subs
43、cribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits contact Membership Administration. Tel: +44 (0)20 8996 7002. Fax: +44 (0)20 8996 7001. Email: membershipbsi-.Informatio
44、n regarding online access to British Standards via British Standards Online can be found at http:/www.bsi- information about BSI is available on the BSI website at http:/www.bsi-.CopyrightCopyright subsists in all BSI publications. BSI also holds the copyright, in the UK, of the publications of the
45、international standardization bodies. Except as permitted under the Copyright, Designs and Patents Act 1988 no extract may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, photocopying, recording or otherwise without prior written permission from BSI
46、.This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained.Details and advice can be obtained from the Copyright & Licensing Manager. Tel: +44 (0)20 8996 7070. Fax: +44 (0)20 8996 7553. Email: copyrightbsi-.
