1、BS ISO23954:2009ICS 71.100.60NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDOil of lime expressed,Persian type (Citruslatifolia Tanaka)This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 March2010 BSI 2010ISB
2、N 978 0 580 56994 4Amendments/corrigenda issued since publicationDate CommentsBS ISO 23954:2009National forewordThis British Standard is the UK implementation of ISO 23954:2009.The UK participation in its preparation was entrusted to TechnicalCommittee AW/54, Essential oils.A list of organizations r
3、epresented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal obligations.BS ISO 239
4、54:2009Reference numberISO 23954:2009(E)ISO 2009INTERNATIONAL STANDARD ISO23954First edition2009-12-15Oil of lime expressed, Persian type (Citrus latifolia Tanaka) Huile essentielle de limette exprime froid, type Perse (Citrus latifolia Tanaka) BS ISO 23954:2009ISO 23954:2009(E) PDF disclaimer This
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8、NT ISO 2009 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body i
9、n the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2009 All rights reservedBS ISO 23954:2009ISO 23954:2009(E) ISO 2009 All rights reserved iiiForew
10、ord ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a techni
11、cal committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of e
12、lectrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to
13、the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for i
14、dentifying any or all such patent rights. ISO 23954 was prepared by Technical Committee ISO/TC 54, Essential oils. BS ISO 23954:2009BS ISO 23954:2009INTERNATIONAL STANDARD ISO 23954:2009(E) ISO 2009 All rights reserved 1Oil of lime expressed, Persian type (Citrus latifolia Tanaka) 1 Scope This Inter
15、national Standard specifies certain characteristics of the oil of lime expressed, Persian type (Citrus latifolia Tanaka) in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated refer
16、ences, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of cont
17、ainers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol
18、ISO 4715, Essential oils Quantitative evaluation of residue on evaporation ISO 4735, Oils of Citrus Determination of CD value by ultraviolet spectrometric analysis ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentatio
19、n in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of lime expressed, Persia
20、n type essential oil of lime expressed, Persian type (Citrus latifolia Tanaka) of the Rutaceae family, expressed or pressed from the peel of the fruit without heat NOTE 1 The principal areas of production are the USA, Mexico, and Brazil. NOTE 2 For information on CAS number, see ISO/TR 210922. BS IS
21、O 23954:2009ISO 23954:2009(E) 2 ISO 2009 All rights reserved4 Requirements 4.1 Appearance Mobile clear liquid. 4.2 Colour Green, yellow-green, or brownish-green. 4.3 Odour Characteristic of the peel of fresh lime. 4.4 Relative density at 20 C, 2020d Minimum: 0,861. Maximum: 0,879. 4.5 Refractive ind
22、ex at 20 C Minimum: 1,476. Maximum: 1,486. 4.6 Optical rotation at 20 C Between +38 and +53. 4.7 Miscibility in 95 % (volume fraction) ethanol at 20 C It shall not be necessary to use more than 3 volumes of 95 % (volume fraction) ethanol (with slight haze permitted) to obtain a clear solution with 1
23、 volume of essential oil. 4.8 Residue on evaporation Minimum: 5,0 % (mass fraction). Maximum: 12,0 % (mass fraction). 4.9 CD value Minimum: 0,24. 4.10 Chromatographic profile Carry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative
24、and characteristic components listed in Table 1. The proportions of each of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. BS ISO 23954:2009ISO 23954:2009(E) ISO 2009 All rights reserved 3Table 1 Chromat
25、ographic profile Component Minimum % Maximum % -Pinene 2,0 2,5 -Pinene 10,0 12,0 Myrcene 1,2 2,0 p-Cymene 0 0,5 Limonene47,0 53,0 -Terpinene 11,0 14,0 Neral 1,0 2,0 Geranial 2,0 3,0 Neryl acetate 0,6 1,3 Geranyl acetate 0,2 0,4 -Caryophellene 0,4 0,6 -Bergamotene 0,8 1,3 -Bisabolene 1,5 2,0 NOTE The
26、 chromatographic profile is normative, contrary to the typical chromatograms given for information in Annex A. 4.11 Flashpoint Information concerning the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 30 ml. NOTE This volume allows each of the tests specified
27、in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 2020d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. BS ISO 23954:2009ISO 23954:2009(E) 4 ISO 2009 All rights reserved6.4 Miscibility in 95 %
28、 (volume fraction) ethanol at 20 C See ISO 875. 6.5 Residue on evaporation Test portion: 3 g. Evaporation time: 6 h. See ISO 4715. 6.6 CD value Maximum value: 315 nm. Sample size: 20 mg. See ISO 4735. 6.7 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking, and s
29、torage See ISO/TR 210 and ISO/TR 211. BS ISO 23954:2009ISO 23954:2009(E) ISO 2009 All rights reserved 5Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of lime expressed, Persian type (Citrus latifolia Tanaka) Peak identification Operating condit
30、ions 1 -Pinene 12 -Bergamotene 2 -Pinene 13 -Bisabolene 3 Myrcene 4 p-Cymene 5 Limonene 6 -Terpinene 7 Neral 8 Geranial 9 Neryl acetate 10 Geranyl Acetate 11 -Caryophellene Column: capillary, silica, 30 m length, 0,2 mm internal diameter Stationary phase: poly(5%diphenyl/95%dimethylsiloxane) (SP-51)
31、 Film thickness: 0,20 m Oven temperature: isothermal at 60C for 8 min and then programming temperature from 60 C to 200 C at a rate of 2 C/min Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1:100 Fig
32、ure A.1 Typical chromatogram taken on an apolar column 1) Example of a product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product. BS ISO 23954:2009ISO 23954:2009(E) 6 ISO 2009 All rights reser
33、vedPeak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 -Pinene -Pinene Myrcene Limonene -Terpinene p-Cymene -Bergamotene -Caryophellene Neral Neryl acetate -Bisabolene Geranial Geranyl acetate Column: capillary, silica, 30 m length, 0,2 mm internal diameter Stationary phase: polye
34、thylene glycol (Supelcowax-101) Film thickness: 0,20 m Oven temperature: isothermal at 60 C for 8 min and then programming temperature from 60 C to 200 C at a rate of 2 C /min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected:
35、 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1:100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 23954:2009ISO 23954:2009(E) ISO 2009 All rights reserved 7Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, an
36、d people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend a single apparatus for standardization
37、purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed for certain equipment is incompatible with the high price of essential oils; as there are several different types of equipment which can be used for the determination, users
38、 cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties. The equipment with which this value was obtained has to be sp
39、ecified. For further information, see ISO/TR 110181. B.2 Flashpoint of the essential oil of lime expressed, Persian type (Citrus latifolia Tanaka) The mean value is +46 C. NOTE Obtained with tag closed cup equipment or equivalent. BS ISO 23954:2009ISO 23954:2009(E) 8 ISO 2009 All rights reservedBibl
40、iography 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 23954:2009BS ISO 23954:2009ISO 23954:2009(E) ICS 71.100.60 Price based on 8 pages ISO 2009 All rights reserved BS ISO 23954:2009This page has been intent
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