1、BSI Standards PublicationBS ISO 25157:2013Essential oil of rose, Chinese Kushui type (Rosa sertata Rosa rugosa)BS ISO 25157:2013 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 25157:2013. The UK participation in its preparation was entrusted to TechnicalCommit
2、tee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 20
3、13. Published by BSI Standards Limited 2013ISBN 978 0 580 80100 6 ICS 71.100.60 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2013.Amendments issued si
4、nce publicationDate T e x t a f f e c t e dBS ISO 25157:2013 ISO 2013Essential oil of rose, Chinese Kushui type (Rosa sertata Rosa rugosa)Huile essentielle de rose, type chinois Kushui (Rosa sertata Rosa rugosa)INTERNATIONAL STANDARDISO25157First edition2013-04-15Reference numberISO 25157:2013(E)BS
5、ISO 25157:2013ISO 25157:2013(E)ii ISO 2013 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2013All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or po
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7、tiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 25157:2013ISO 25157:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
8、ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates
9、 closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval crite
10、ria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2, www.iso.org/directives.Attention is drawn to the possibility that some of the elements of this document may be the subject of paten
11、t rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received, www.iso.org/patents.Any trade name used in thi
12、s document is information given for the convenience of users and does not constitute an endorsement.The committee responsible for this document is ISO/TC 54, Essential oils. ISO 2013 All rights reserved iiiBS ISO 25157:2013BS ISO 25157:2013Essential oil of rose, Chinese Kushui type (Rosa sertata Ros
13、a rugosa)1 ScopeThis International Standard specifies certain characteristics of the essential oil of rose, Chinese Kushui type (Rosa sertata Rosa rugosa) cultivated mainly in China, in order to facilitate assessment of its quality.2 Normative referencesThe following documents, in whole or in part,
14、are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO/TR 210, Essential oils General rules for packaging
15、, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils
16、 Determination of optical rotationISO 709, Essential oils Determination of ester valueISO 1041, Essential oils Determination of freezing pointISO 11024 (all parts), Essential oils General guidance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following term
17、s and definitions apply.3.1essential oil of rose, Chinese Kushui typeessential oil obtained by steam distillation of the flowers of Rosa sertata Rosa rugosa cultivated mainly in ChinaNote 1 to entry: For information on the CAS number, see ISO/TR 21092.24 Requirements4.1 AppearanceLiquid or more or l
18、ess crystallized.4.2 ColourLight yellow or yellow brown.INTERNATIONAL STANDARD ISO 25157:2013(E) ISO 2013 All rights reserved 1BS ISO 25157:2013ISO 25157:2013(E)4.3 OdourCharacteristic, full-bodied, floral, rose.Relative density at 20 C d2020Minimum: 0,880.Maximum: 0,930.4.4 Refractive index at 20 C
19、Minimum: 1,4680.Maximum: 1,4798.4.5 Optical rotation at 20 CBetween 10,3 and 0,5.4.6 Freezing pointBetween 11 C and 14 C.4.7 Ester valueMinimum: 18.Maximum: 24.4.8 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained the repr
20、esentative and characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil.4.9 FlashpointInformation on the flashpoint is given in Annex B.5 SamplingSampl
21、ing shall be performed in accordance with ISO 212.Minimum volume of test sample: 25 ml.NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.2 ISO 2013 All rights reservedBS ISO 25157:2013ISO 25157:2013(E)Table 1 Chromatographic profileCom
22、ponentsMinimum %Maximum %Ethanol n.d.a1,0cis-Rose oxide n.d.a0,5Linalool 1,0 3,5-Phenylethyl alcohol n.d.a0,3Citronellol 40,0 50,0Nerol 2,0 5,5Geraniol 6,0 18,0Citronellyl acetate 2,5 4,5Eugenol methyl ether 0,8 2,0Farnesol 2,0 3,5Heneicosane (paraffin C21) 0,6 2,0Tricosane (paraffin C23) 0,6 2,0NOT
23、E The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A.a Not detectable.6 Test methodsRelative density at 20 C d2020Determine the relative density in accordance with ISO 279.6.1 Refractive index at 20 CDetermine the refractive index in accordan
24、ce with ISO 280.6.2 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592.6.3 Freezing pointDetermine the freezing point in accordance with ISO 1041.6.4 Ester valueDetermine the ester value in accordance with ISO 709.6.5 Chromatographic profileDetermine the chromatographi
25、c profile in accordance with ISO 11024.7 Packaging, labelling, marking and storageThese items shall be in accordance with ISO/TR 210 and ISO/TR 211. ISO 2013 All rights reserved 3BS ISO 25157:2013ISO 25157:2013(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatographic of e
26、ssential oil of rose, Chinese Kushui type (Rosa sertata Rosa rugosa)The chromatogram in Figure A.1 results from the use of a medium polar column and that in Figure A.2 of a polar column.4 ISO 2013 All rights reservedBS ISO 25157:2013ISO 25157:2013(E)Peak identification Operating conditions1 Ethanol
27、Column: fused capillary silica, 60 m length, 0,20 mm internal diameter2 -Pinene Stationary phase: 7 %cyanic-propyl, 7 %phenyl, polydimethyllsiloxane (OV1701a)3 Heptanal Film thickness: 0,33 m4 (Z)-Rose oxide Oven temperature: isothermal at 60 C for 1 min, then programming temperature from 60 C to 27
28、0 C at a rate of 2 C/min, isothermal at 270 C for 10 min5 Linalool Injector temperature: 260 C6 Benzyl alcohol Detector temperature: 260 C7 -Phenylethyl alcohol Detector: flame ionization type8 -Terpineol Carrier gas: nitrogen9+10 Citronellol + Nerol Volume injected: 1,0 l11 Geraniol Carrier gas flo
29、w rate: 0,8 ml/min12 Geranial Split ratio: 1/10013 Citronellyl acetate14 Eugenol15 Eugenol methyl ether16 Farnesol17 Heneicosane (Paraffin C21)18 Tricosane (Paraffin C23) t time19 Pentacosane (Paraffin C25)a Product available commercially. This information is given for the convenience of users of th
30、is document and does not constitute an endorsement by ISO of this product.Figure A.1 Typical chromatogram taken on a medium polar column ISO 2013 All rights reserved 5BS ISO 25157:2013ISO 25157:2013(E)Peak identification Operating conditions1 Ethanol Column: fused capillary silica, 60 m length, 0,25
31、 mm internal diameter2 -Pinene Stationary phase: polyethylene glycol (HP-Innowaxa)3 Heptanal Film thickness: 0,50 m4 2-Heptanol Oven temperature: isothermal at 60 C for 1 min, then programming temperature from 60 C to 255 C at a rate of 2 C/min, isothermal at 255 C for 20 min5 (Z)-Rose oxide6 Linalo
32、ol Injector temperature: 260 C7 Citronellyl acetate Detector temperature: 260 C8 Neral Detector: flame ionization type9 -Terpineol Carrier gas: nitrogen10 Neryl acetate Volume injected: 0,6 l11 Geranial Carrier gas flow rate: 0,8 ml/min12 Geranyl acetate Split ratio: 1/10013 Citronellol14 Nerol15 2-
33、Tridecanone16 Geraniol17 Nonadecane18 Benzyl alcohol19 Eugenol methyl ether20 Heneicosane21 Eugenol22 Tricosane t time23 Farnesola Product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Fig
34、ure A.2 Typical chromatogram taken on a polar column6 ISO 2013 All rights reservedBS ISO 25157:2013ISO 25157:2013(E)Annex B (informative) FlashpointB.1 General informationFor safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the
35、flashpoints of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical co
36、mposition of essential oils; the volume of the sample needed in certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it
37、 was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties.The equipment with which this value was obtained has to be specified.For further information see ISO/TR 11018.1B.2 Flashpoint of t
38、he essential oil of rose, Chinese Kushui typeThe mean value is +61 C.NOTE Obtained with a semi-automatic closed cup flash tester NPM221.1)1) Equipment available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this
39、 product. ISO 2013 All rights reserved 7BS ISO 25157:2013ISO 25157:2013(E)Bibliography1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint2 ISO/TR 21092, Essential oils Characterization8 ISO 2013 All rights reservedBS ISO 25157:2013BS ISO 25157:2013ISO 25157:2013(E) ISO
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