1、BRITISH STANDARD BS ISO 3045:2004 Oil of bay Pimenta racemosa (Mill.) J.W. Moore ICS 71.100.60 BS ISO 3045:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 2 June 2004 BSI 2 June 2004 ISBN 0 580 43840 6 National foreword This British Stan
2、dard reproduces verbatim ISO 3045:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on request
3、to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalo
4、gue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand th
5、e text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a fron
6、t cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments Referen
7、ce number ISO 3045:2004(E)NINRETOITALAN ADNATSDR OSI 3540 ceSond deition -400251-50 Oil of bay Pimenta racemosa (Mill.) J.W. Moore Huile essentielle de bay Pimenta racemosa (Mill.) J.W. Moore Referecne unbmer OSI :5403)E(4002 OSI 4002INTERNATIONAL STANDARD ISO 3045 Second edition 2004-05-15 o liOf b
8、ay iPemecar atnomas ).lliM( W.J. erooM iuHlse esenteilld ee yab iPmr atneacemosa (Mill.) J.W. Mrooe BSISO3045:2004 ii BSISO3045:2004 iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing
9、International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaiso
10、n with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of tec
11、hnical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is dra
12、wn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 3045 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the
13、first edition (ISO 3045:1974), which has been technically revised. BSISO3045:2004blankINTENRATIONAL TSANDADR IS:5403 O4002(E)1Oil of bay Pimenta racemosa (Mill.) J.W. Moore 1 Scope This International Standard specifies certain characteristics of the oil of bay 1)Pimenta racemosa (Mill.) J.W. Moore,
14、in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispen- sable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including
15、 any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference met
16、hod ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 1272, Essential oils Determination of phenols content ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles f
17、or presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 1)Also commercially known as “Saint-Thomas bay”. 3 Terms and definitions For the purposes of this document, the following
18、 terms and definitions apply. 3.1 oil of bay essential oil obtained by steam distillation of the leaves of Pimenta racemosa (Mill.) J.W. Moore, of the Myrtaceae family. NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear mobile liquid. 4.2 Colour Dark brown.
19、 4.3 Odour Characteristic, spicy. 4.4 Flavour Spicy, hot. 4.5 Relative density at 20 C, 20 20 d Minimum: 0,943 Maximum: 0,984 4.6 Refractive index at 20 C Minimum: 1,505 Maximum: 1,517 4.7 Optical rotation at 20 C Between 0,5 and + 0,5. BSISO3045:20042 Since the product is highly coloured, it is str
20、ongly recommended to decolorize it with an appropriate bleach, excluding distillation, before determination of the optical rotation. 4.8 Content of phenols Minimum: 52 % (volume fraction) 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chr
21、omatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato- graphic profile of the essential oil. Table 1 Chromatographic prof
22、ile Component Minimum % Maximum % Myrcene 20,0 30,0 Limonene a1,0 4,0 Linalol 1,0 3,0 Methyl eugenol 0,1 2,0 Eugenol 42,0 56,0 Chavicol 8,0 13,0 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. aThis is considered to be completely D-l
23、imonene by independent chemical and physical analysis. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.
24、6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Content of phenols See ISO 1272. 6.5 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packing, labelling, marking and storage See ISO/TR
25、 210 and ISO/TR 211. BSISO3045:2004 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of bay Pimenta racemosa (Mill.) J.W. Moore Peak identification 1 Myrcene 2 Limonene 3 Linalol 4 Chavicol 5 Eugenol 6 Methyl eugenol Operating conditions Column:
26、 silica capillary; length 20 m; internal diameter 100 m Stationary phase: methyl siloxane Film thickness: 0,4 m Oven temperature: isothermal at 50 C for 1 min, then temperature programming from 50 C to 220 C at a rate of 10 C/min, and isothermal at 220 C for 13 min Injector temperature: 250 C Detect
27、or temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0,3 ml/min Pressure: start at 220,63 kPa for 20 min, then 34,47 kPa/min up to 310,26 kPa, then 310,26 kPa for 20 min Figure A.1 Typical chromatogram taken on an apolar column BSI
28、SO3045:20044 Peak identification 1 Myrcene 2 Limonene 3 Linalol 4 Methyl eugenol 5 Eugenol 6 Chavicol Operating conditions Column: silica capillary; length 20 m; internal diameter 100 m Stationary phase: poly(ethylene glycol) 20 000 (Carbowax 20 M) Film thickness: 0,2 m Oven temperature: isothermal
29、at 50 C for 1 min, then temperature programming from 50 C to 220 C at a rate of 10 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0,3 ml/min Pressure programming: start at 220,63 kPa fo
30、r 20 min, then increase at 34,47 kPa/min up to 310,26 kPa, then 310,26 kPa for 20 min Figure A.2 Typical chromatogram taken on a polar column BSISO3045:2004 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of
31、 safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that:
32、there is wide variation in the chemical compo- sition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to
33、 use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further in
34、formation, see ISO/TR 11018. B.2 Flashpoint of the essential oil of bay The mean value is + 64 C. NOTE Obtained with “Luchaire” equipment. BSISO3045:20046 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092: 2) , Essential oils Characte
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