1、BS ISO 3525:2008ICS 71.100.60NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDOil of amyris (Amyrisbalsamifera L.)This British Standard was published under theauthority of the StandardsPolicy and StrategyCommittee on 32008 BSI 2008ISBN 978 0 580 57078 0Amendments
2、/corrigenda issued since publicationDate CommentsBS ISO 3525:2008National forewordThis British Standard is the UK implementation of ISO 3525:2008.The UK participation in its preparation was entrusted to TechnicalCommittee AW/54, Essential oils.A list of organizations represented on this committee ca
3、n be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal obligations.0 SeptemberBS ISO 3525:2008Reference numb
4、erISO 3525:2008(E)ISO 2008INTERNATIONAL STANDARD ISO3525Second edition2008-07-15Oil of amyris (Amyris balsamifera L.) Huile essentielle damyris (Amyris balsamifera L.) BS ISO 3525:2008ISO 3525:2008(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing po
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9、neva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2008 All rights reservedBS ISO 3525:2008ISO 3525:2008(E) ISO 2008 All rights reserved iiiForeword ISO (the International Organization for Standardization) is a worldwide feder
10、ation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that commit
11、tee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordanc
12、e with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires a
13、pproval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 3525 was prepared by Technical Comm
14、ittee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 3525:1979), which has been technically revised. BS ISO 3525:2008BS ISO 3525:2008INTERNATIONAL STANDARD ISO 3525:2008(E) ISO 2008 All rights reserved 1Oil of amyris (Amyris balsamifera L.) 1 Scope This In
15、ternational Standard specifies certain characteristics of essential oil of amyris (Amyris balsamifera L.), with a view to facilitating the assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references
16、, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of container
17、s ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1
18、242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chrom
19、atographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of amyris essential oil obtained by steam distillation of the wood of Amyris balsamifera L. of the Rutaceae family mainly distilled
20、 in Haiti NOTE For information on the CAS number, see ISO/TR 210922. 4 Requirements 4.1 Appearance Clear, slightly viscous liquid. 4.2 Colour Pale yellow to amber yellow. 4.3 Odour Characteristic, woody. 4.4 Relative density at 20 C, 2020d Minimum: 0,946. Maximum: 0,978. 4.5 Refractive index at 20 C
21、 Minimum: 1,504. Maximum: 1,512. 4.6 Optical rotation at 20 C Between +20 and +45. BS ISO 3525:2008ISO 3525:2008(E) 2 ISO 2008 All rights reserved4.7 Miscibility with 80 % (volume fraction) ethanol at 20 C It shall not be necessary to use more than 3 volumes of 80 % (volume fraction) ethanol to obta
22、in a clear solution with 1 volume of essential oil. Sometimes opalescence can be observed on further addition of solvent. 4.8 Acid value Maximum: 3,0. 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representativ
23、e and characteristic constituents listed in Table 1 shall be identified. The percentages of these constituents, indicated by the integrator, shall be situated within the limits given in Table 1. This constitutes the chromatographic profile of the essential oil. 4.10 Flashpoint Information on the fla
24、shpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume is sufficient for each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 2020d See ISO 279. 6.2 Refractive ind
25、ex at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility with 80 % (volume fraction) ethanol at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking, and storage See ISO/TR 210 and ISO/TR
26、211. Table 1 Chromatographic profile Minimum Maximum Component % % Elemol 5,0 15,0 10-epi-Eudesmol 6,0 12,0 -Eudesmol 4,0 12,0 Valerianol 15,0 35,0 -Eudesmol 3,0 9,0 7-epi-Eudesmol 7,0 15,0 -Eudesmol 2,5 11,0 Drimenol 0,7 5,0 NOTE The chromatographic profile is normative, contrary to typical chromat
27、ograms given for information in Annex A. BS ISO 3525:2008ISO 3525:2008(E) ISO 2008 All rights reserved 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of amyris (Amyris balsamifera L.) BS ISO 3525:2008ISO 3525:2008(E) 4 ISO 2008 All rights rese
28、rvedPeak identification Operating conditions 1 Elemol 2 10-epi-Eudesmol 3 -Eudesmol 4 -Eudesmol 5 Valerianol 6 -Eudesmol 7 7-epi-Eudesmol 8 Drimenol Column: silica capillary; length 30 m; internal diameter, 320 m Stationary phase: poly(5% phenyl)(95 % methyl)siloxane HP-51) Film thickness: 0,25 m Ov
29、en temperature: isothermal at 70 C during 10 min, then programming of temperature from 70 C to 220 C, at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 1,1 ml/min Split rati
30、o: 1/100 Figure A.1 Typical chromatogram taken on an apolar column 1) HP-5 is an example of a suitable product available commercially. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. Equivalent produ
31、cts may be used if they can be shown to lead to the same results. BS ISO 3525:2008ISO 3525:2008(E) ISO 2008 All rights reserved 5Peak identification Operating conditions 1 Elemol 2 10-epi-Eudesmol 3 -Eudesmol 4 Valerianol 5 -Eudesmol 6 -Eudesmol 7 7-epi-Eudesmol 8 Drimenol Column: silica capillary;
32、length 20 m; internal diameter 100 m Stationary phase: poly(ethylene glycol) Carbowax 20 M2) Film thickness: 0,2 m Oven temperature: isothermal at 50 C for 1 min, then programming of temperature from 50 C to 200 C, at a rate of 10 C/min Injector temperature: 230 C Detector temperature: 250 C Detecto
33、r: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 1,1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column 2) Carbowax 20M is an example of a suitable product available commercially. This information is given for the convenience
34、 of users of this International Standard and does not constitute an endorsement by ISO of this product. Equivalent products may be used if they can be shown to lead to the same results. BS ISO 3525:2008ISO 3525:2008(E) 6 ISO 2008 All rights reservedAnnex B (informative) Flashpoint B.1 General inform
35、ation For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018 1) concluded tha
36、t it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for highly priced essential oils; as there are several dif
37、ferent types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the intereste
38、d parties. The equipment with which this value was obtained has to be specified. For further information see ISO/TR 110181. B.2 Flashpoint of the essential oil of amyris (Amyris balsamifera L.) The mean value is more than 100 C. NOTE Obtained with “Pensky-Martens” 3)equipment. 3) Equipment available
39、 commercially. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. BS ISO 3525:2008ISO 3525:2008(E) ISO 2008 All rights reserved 7Bibliography 1 ISO/TR 11018, Essential oils General guidance on the deter
40、mination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 3525:2008ISO 3525:2008(E) ICS 71.100.60 Price based on 7 pages ISO 2008 All rights reserved BS ISO 3525:2008This page has been intentionally left blank BS ISO3525:2008BSI GroupHeadquarters 389Chiswick High Road,London, W4
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