1、BRITISH STANDARD BS ISO 4725:2004 Oil of cedarwood, Texas (Juniperus mexicana Schiede) ICS 71.100.60 BS ISO 4725:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2004 BSI 31 May 2004 ISBN 0 580 43838 4 National foreword This Britis
2、h Standard reproduces verbatim ISO 4725:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on re
3、quest to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic
4、Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to underst
5、and the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises
6、a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments R
7、eference number ISO 4725:2004(E) OSI 4002INTERNATIONAL STANDARD ISO 4725 Second edition 2004-05-15 Oil of cedarwood, Texas (Juniperus mexicana Schiede) Huile essentielle de bois de cdre, Texas (Juniperus mexicana Schiede) BSISO4725:2004IS:5274 O4002(E) DPlcsid Fremia ihTs PDF file may ctnoian emdebt
8、 dedyfepcaes. In ccaocnadrw eith Aebods licensilop gnic,y this file mairp eb ynted iv roweb detu slahl ton ide ebtlnu deess the typefaces whice era hml era deddebicsnede to i dnanstlaled t noeh computfrep reormign tide ehtin.g In wodlnidaot gnhis file, trapise atpecc tiereht nser ehnopsiiblity fo no
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10、tpo erimizde for irpnti.gn Evyre caer neeb sah taken to sneeru that the file is suitlbae fosu re yb ISO memdob rebeis. In tlnu ehikletneve y ttah lborp aem leratit gno it is f,dnuo plsaee inform ttneC ehlar Secrteiraat ta the serddaig sleb nevwo. ISO 4002 All irthgs erse.devr lnUeto sswrehise specif
11、ied, on trap fo this lbupictaion maeb y cudorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mecinahcal, inclidung tohpcoiypodna gn micrfoilm, wittuoh repmissii non writign from ietI rehSa Ot tsserdda eh ebolw or ISOs memreb i ydobn the cnuotrfo y ttseuqer ehe.r ISO cirypothg fofice saC
12、e tsopale 65 eneG 1121-HC 02 av leT. 4 + 10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mail coirypthgiso.o gr We bwww.is.o gro Pulbisdehi n Switlrez dnaii ISO 4002 Allr ithgsr esedevrBSISO4725:2004IS:5274 O4002(E) I SO 4002 All irthgs ersedevr iiiForeword ISO (the International Organization for Standardiza
13、tion) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be
14、represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standard
15、s are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Interna
16、tional Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 4725 was
17、prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 4725:1986), which has been technically revised. BSISO4725:2004blank 4002:5274OSISBINTENRATIONAL TSANDADR IS:5274 O4002(E)I SO 4002 All irthgs ersedevr 1Oil of cedarwood, Texas (
18、Juniperus mexicana Schiede) 1 Scope This International Standard specifies certain characteristics of the oil of cedarwood, Texas (Juniperus mexicana Schiede), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispen- sable for the appli
19、cation of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General
20、 rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oi
21、ls Evaluation of miscibility in ethanol ISO 3794, Essential oils (containing tertiary alcohols) Estimation of free alcohols content by determination of ester value after acetylation ISO 7660, Essential oils Determination of ester value of oils containing difficult-to-saponify esters ISO 11024-1, Ess
22、ential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and def
23、initions For the purposes of this document, the following terms and definitions apply. 3.1 oil of cedarwood, Texas essential oil obtained by steam distillation of the wood of Juniperus mexicana Schiede, from the west of North America and Central America, of the Cupressaceae family NOTE For informati
24、on on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Viscous liquid. 4.2 Colour Brown to reddish brown. 4.3 Odour Characteristic, woody. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,950 Maximum: 0,966 4.5 Refractive index at 20 C Minimum: 1,502 Maximum: 1,510 BSISO4725:2004IS:52
25、74 O4002(E) 2 I SO 4002 All irthgs ersedevr4.6 Optical rotation at 20 C Between 52 and 30 4.7 Miscibility in ethanol, 90 % (volume fraction), at 20 C It shall not be necessary to use more than 5 volumes of ethanol, 90 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.
26、8 Content of alcohols, expressed as cedrol Minimum: 35 % (volume fraction) Maximum: 48 % (volume fraction) 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Tab
27、le 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato- graphic profile of the essential oil. Table 1 Chromatographic profile Component Minimum Maximum % % -Cedrene 15,0 25,0 -Cedrene 3,0 8,0 Thujopsene
28、25,0 35,0 Cuparene 1,5 3,2 Cedrol 20,0 Widdrol 2,5 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE
29、This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 90 % (volum
30、e fraction), at 20 C See ISO 875. 6.5 Content of alcohols, expressed as cedrol See ISO 3794. Saponification shall be carried out according to ISO 7660. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO4725:20
31、04IS:5274 O4002(E) I SO 4002 All irthgs ersedevr 3Annex A (informative) Typical chromatogram of the analysis by gas chromatography of the essential oil of cedarwood, Texas (Juniperus mexicana Schiede) Peak identification Operating conditions 1 -Cedrene 2 -Cedrene 3 Thujopsene 4 Cuparene 5 Cedrol 6 W
32、iddrol Column: silica capillary; length 30 m; internal diameter 320 m Stationary phase: poly(dimethyl siloxane) (HP-5) Film thickness: 0,25 m Oven temperature: isothermal at 70 C for 10 min, then programming from 70 C to 220 C, at a rate of 2 C/min Injector temperature: 250 C Detector temperature: 2
33、50 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,2 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BSISO4725:2004IS:5274 O4002(E) 4 I SO 4002 All irthgs ersedevrPeak identification Operating conditions 1 -Ced
34、rene 2 -Cedrene 3 Thujopsene 4 Cuparene 5 Cedrol 6 Widdrol Column: silica capillary; length 20 m; internal diameter100 m Stationary phase: poly(ethylene glycol) (Carbowax-20 M) Film thickness: 0,25 m Oven temperature: isothermal at 50 C for 1 min, then programming from 50 C to 200 C, at a rate of 10
35、 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0,3 ml/min Pressure programming: start at 220,68 kPa for 20 min, then increasing at 34,48 kPa/min up to 310,34 kPa, then 310,34 kPa for 2
36、0 min Figure A.2 Typical chromatogram taken on a polar column BSISO4725:2004IS:5274 O4002(E) I SO 4002 All irthgs ersedevr 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require informati
37、on on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for stan- dardization purposes, given that: there is a wide variation in the
38、 chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; as there are several different types of equip- ment which can be used for the determination, users cannot be expected to use one specified type only.
39、Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B
40、.2 Flashpoint of the essential oil of cedarwood, Texas The mean value is +107 C. NOTE Obtained with “Luchaire” equipment. BSISO4725:2004IS:5274 O4002(E) 6 I SO 4002 All irthgs ersedevrBibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092:
41、 1) , Essential oils Characterization 1)To be published. BSISO4725:2004blankBS ISO 4725:2004 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and
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