1、BRITISH STANDARD BS ISO 4728:2003 Oil of Spanish wild marjoram (Thymus mastichina L.) ICS 71.100.60 BS ISO 4728:2003 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 16 September 2003 BSI 16 September 2003 ISBN 0 580 42638 6 National foreword
2、This British Standard reproduces verbatim ISO 4728:2003 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obt
3、ained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI
4、Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers
5、 to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document
6、 comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments Reference nu
7、mber ISO 4728:2003(E) OSI 3002INTERNATIONAL STANDARD ISO 4728 Second edition 2003-09-01 Oil of Spanish wild marjoram (Thymus mastichina L.) Huile essentielle de marjolaine sauvage dEspagne (Thymus mastichina L.) BSISO4728:2003IS:8274 O3002(E) DPlcsid Fremia ihTs PDF file may ctnoian emdebt dedyfepca
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11、n trap fo this lbupictaion maeb y cudorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mecinahcal, inclidung tohpcoiypodna gn micrfoilm, wittuoh repmissii non writign from ietI rehSa Ot tsserdda eh ebolw or ISOs memreb i ydobn the cnuotrfo y ttseuqer ehe.r ISO cirypothg fofice saCe tsop
12、ale 65 eneG 1121-HC 02 av leT. 4 + 10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mail coirypthgiso.o gr We bwww.is.o gro Pulbisdehi n Switlrez dnaii ISO 3002 Allr ihgtsser edevrBSISO4728:2003IS:8274 O3002(E) I SO 3002 All irhgts seredevr iiiForeword ISO (the International Organization for Standardization)
13、is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be repres
14、ented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Interna- tional Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards ar
15、e drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Interna- ti
16、onal Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 4728 was pr
17、epared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 4728:1992), which has been technically revised. BSISO4728:2003blank BSISO4728:2003INTENRATIONAL TSANDADR IS:8274 O3002(E)I SO 3002 All irhgts seredevr 1Oil of Spanish wild marjora
18、m (Thymus mastichina L.) 1 Scope This International Standard specifies certain charac- teristics of the oil of Spanish wild marjoram (Thymus mastichina L.), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispen- sable for the applica
19、tion of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the refer- enced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General
20、 rules for la- belling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refrac- tive index ISO 356, Essential oils Preparation of test sam- ples ISO 592, Essential oi
21、ls Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in stan- da
22、rds ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of Spanish wild marjoram essential o
23、il obtained by steam distillation of the flowering tops of Thymus mastichina L., of the Lamiaceae family, growing in different parts of Spain NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Liquid. 4.2 Colour Almost colourless to yellow. 4.3 Odour Characterist
24、ic, cineolic and spicy. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,890 Maximum: 0,920 4.5 Refractive index at 20 C Minimum: 1,460 Maximum: 1,470 BSISO4728:2003IS:8274 O3002(E) 2 I SO 3002 All irhgts seredevr4.6 Optical rotation at 20 C Between 4 and +6. 4.7 Miscibility in ethanol, 70 % (volume
25、 fraction), at 20 C It shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. NOTE Sometimes opalescence is observed on dilu- tion. 4.8 Acid value Maximum: 2 4.9 Chromatographic profile Analysis of the essentia
26、l oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic
27、profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 1 4,5 -Pinene 2 5 Limonene 1 6 1,8-Cineole 30 68 Linalol 3 48 Camphor 0,1 2 -Terpineol 0,2 2 Borneol 0,1 1,8 Terpinen-4-ol 0,2 1,2 Linalyl acetate 0,2 4 -Caryophyllene 0,5 1,5 NOTE The chromatographic
28、 profile is normative, contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests speci- fied in this International Stan
29、dard to be carried out at least once. 6 Preparation of test sample See ISO 356. 7 Test methods 7.1 Relative density at 20 C, 20 20 d See ISO 279. 7.2 Refractive index at 20 C See ISO 280. 7.3 Optical rotation at 20 C See ISO 592. 7.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C See ISO 87
30、5. 7.5 Acid value See ISO 1242. 7.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 8 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO4728:2003IS:8274 O3002(E) I SO 3002 All irhgts seredevr 3Annex A (informative) Typical chromatograms of the analysis by gas chr
31、omatography of the essential oil of Spanish wild marjoram (Thymus mastichina L.) BSISO4728:2003IS:8274 O3002(E) 4 I SO 3002 All irhgts seredevrPeak identification Operating conditions 1 -Pinene 2 Camphene 3 Sabinene 4 -Pinene 5 Myrcene 6 p-Cymene 7 Limonene + 1,8-cineole 8 Linalol 9 Camphor 10 Borne
32、ol + -terpineol 11 Terpinen-4-ol 12 -Terpineol 13 Linalyl acetate 14 -Caryophyllene Column: fused silica capillary; length 60 m; internal diameter 0,25 mm Stationary phase: poly(5 % diphenyl 95 % dimethylsiloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 75 C to 190 C at
33、 a rate of 4 C/min Injector temperature: 250 C Detector temperature: 270 C Detector: flame ionization type Carrier gas: nitrogen Injection volume: 0,6 l Carrier gas flow rate: 1 ml/min Split ratio: 1/80 Figure A.1 Typical chromatogram taken on an apolar column BSISO4728:2003IS:8274 O3002(E) I SO 300
34、2 All irhgts seredevr 5Peak identification Operating conditions 1 -Pinene 2 Camphene 3 -Pinene 4 Sabinene 5 Myrcene 6 Limonene 7 1,8-Cineole 8 Linalol 9 Linalyl acetate + camphor 10 -Caryophyllene 11 Terpinen-4-ol 12 -Terpineol 13 -Terpineol 14 Borneol Column: fused silica capillary; length 60 m; in
35、ternal diameter 0,25 mm Stationary phase: poly(ethylene glycol)-modified TPA (SP-1 000) Film thickness: 0,25 m Oven temperature: temperature programming from 95 C to 190 C at a rate of 4 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: nitrog
36、en Injection volume: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BSISO4728:2003IS:8274 O3002(E) 6 I SO 3002 All irhgts seredevrAnnex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insura
37、nce companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for
38、standardization purposes, given that: there is wide variation in the chemical composi- tion of essential oils; the volume of the sample needed for certain requirements would be too costly for high- priced essential oils; as there are several different types of equip- ment which can be used for the d
39、etermination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, for information, in order to meet the requirements of the interested parties. The equipment with which t
40、his value was obtained should be specified. For further information see ISO/TR 11018. B.2 Flashpoint of the essential oil of Spanish wild marjoram The mean value is +59 C. NOTE Obtained with “Setaflash” equipment. BSISO4728:2003IS:8274 O3002(E) I SO 3002 All irhgts seredevr 7Bibliography 1 ISO/TR 11
41、018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092: 1) , Essential oils Characterization 1) To be published. BSISO4728:2003BS ISO 4728:2003 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body respons
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