1、PUBLISHED DOCUMENT PD CEN/TR 14839:2004 Wood preservatives Determination of the preventive efficacy against wood destroying basidiomycetes fungi ICS 71.100.50 PD CEN/TR 14839:2004 This Published Document was published under the authority of the Standards Policy and Strategy Committee on 1 April 2004
2、 BSI 1 April 2004 ISBN 0 580 43592 X National foreword This Published Document is the official English language version of CEN/TR 14839:2004. The UK participation in its preparation was entrusted by Technical Committee B/515, Wood preservation, to Subcommittee B/515/4, Assessment of preservation eff
3、icacy, which has the responsibility to: A list of organizations represented on this subcommittee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue u
4、nder the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct a
5、pplication. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summa
6、ry of pages This document comprises a front cover, an inside front cover, the CEN/TR title page, pages 2 to 28, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Co
7、mmentsTECHNICALREPORT RAPPORTTECHNIQUE TECHNISCHERBERICHT CEN/TR14839 February2004 ICS71.100.50 Englishversion WoodpreservativesDeterminationofthepreventiveefficacy againstwooddestroyingbasidiomycetesfungi ProduitsdeprservationduboisDterminationde lefficacitprventivevisvisdeschampignonslignivores ba
8、sidiomyctes HolzschutzmittelBestimmungdervorbeugendenWirkung gegenholzzerstrendeBasidiomyceten ThisTechnicalReportwasapprovedbyCENon12November2003.IthasbeendrawnupbytheTechnicalCommitteeCEN/TC38. CENmembersarethenationalstandardsbodiesofAustria,Belgium,Cyprus,CzechRepublic,Denmark,Estonia,Finland,Fr
9、ance, Germany,Greece,Hungary,Iceland,Ireland,Italy,Latvia,Lithuania,Luxembourg,Malta,Netherlands,Norway,Poland,Portugal, Slovakia, Slovenia,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStas
10、sart,36B1050Brussels 2004CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.CEN/TR14839:2004:ECEN/TR14839:2004(E) 2 Contents Page 1 Scope 5 2 Reference5 3 Termsanddefinitions. .5 4 Principle5 5 Testmaterialsandapparatus .5 5.1 Biologicalmaterial . 5 5.2
11、Feederblocks. 6 5.3 Productsandreagents. .6 5.4 Apparatus .8 6 Samplingofthepreservative 9 7 Testspecimens 9 7.1 Speciesofwood. 9 7.2 Woodquality .9 7.3 Provisionofthetestspecimens. 9 7.4 Dimensionsoftestspecimens .9 7.5 Numberoftestsspecimens. .10 8 Procedure .10 8.1 Preparationoftestspecimens. .10
12、 8.2 Preparationoftestcontainers 10 8.3 Exposureoftestspecimensincontainers 11 8.4 Cultureconditionsanddurationoftest . .11 8.5 Assessmentoffungaldevelopmentinthetestspecimens 12 9 Validityoftest .12 10 Calculationofresults . .12 11 Testreport 13 AnnexAExampleofatestreport . 17 AnnexBTestfungi . 19
13、AnnexCMethodsofsterilization . .22 AnnexDDeterminationofwaterholdingcapacity(WHC) 23 AnnexEOptionalmethodofassessmentofcolonizationofthetestspecimensbythetestfungi 24 Bibliography 28CEN/TR14839:2004(E) 3 Foreword Thisdocument(CEN/TR14839:2004)hasbeenpreparedbyTechnicalCommitteeCEN/TC38“Durabilityofw
14、ood andwoodbasedproducts”, thesecretariatofwhichisheldbyAFNOR. ThestatusofthisdocumentasTechnicalReporthasbeenchosenbecauseithasbeenjudgedusefultomaintain thisoldmethodformerENV839:1993supersededbyENV839:2002fortheuseoflaboratories.CEN/TR14839:2004(E) 4 Introduction ThisTechnicalReportspecifiesalabo
15、ratorymethodoftestwhichgivesabasisforassessingtheeffectivenessofa woodpreservative,whenappliedmainlyasasurfacetreatment,againstbasidiomycetesfungi.Incontrastthe methodfordeterminingthetoxicvaluesagainstwoodrottingfungi(EN113)providesameanofdeterminingthe loadingatwhichimpregnatedwoodofasusceptiblesp
16、eciescanberegardedasadequatelyprotectedunderthe testconditions. Thislaboratorymethodprovidesonecriterionbywhichtheeffectivenessofaproductcanbeassessed.Inmaking thisassessmentthemethodsbywhichthepreservativemaybeappliedshouldbetakenintoaccount.Itisalso recommendedthatresultsfromthistestshouldbesupple
17、mentedbythosefromotherrelevanttestsandaboveall bypracticalexperience. Theproceduresdescribedinthisstandardmethodareintendedtobecarriedoutbysuitablytrainedand/or supervisedspecialists.AppropriatesafetyprecautionsshouldbeobservedthroughouttheuseofthisTechnical Report.CEN/TR14839:2004(E) 5 1Scope ThisT
18、echnicalReportspecifiesamethodoftestforthedeterminationofthepreventiveactionofawood preservativeagainstbasidiomycetesfungiwhenthepreservativeisappliedasasurfacetreatmenttowood. Thismethodisapplicabletoformulationsofpreservativesinareadytouseformas: waterinsolublechemicalswhicharebeingstudiedasactive
19、fungicides;or organicformulations,assuppliedoraspreparedinthelaboratorybydilutionofconcentrates;or organicwaterdispersibleformulationsassuppliedoraspreparedinthelaboratorybydilutionofconcentrates; or watersolublematerials,forexamplesalts. NOTE Thismethodcanbeusedinconjunctionwithanappropriateageingp
20、rocedure,forexampleEN73. 2Reference ENISO3696, WaterforanalyticallaboratoryuseSpecificationandtestmethods (ISO3696:1987). 3 Termsanddefinitions ForthepurposesofthisTechnicalReport,thefollowingtermsanddefinitionsapply. 3.1 representativesample samplehavingitsphysicalorchemicalcharacteristicsidentical
21、tothevolumetricaveragecharacteristicsofthetotal volumebeingsampled 3.2 supplier sponsorofthetest 3.3 superficialapplicationprocess processwhichdoesnotincludeparticularfeaturesorproceduresintendedtoovercomethenaturalresistanceof woodtopenetrationbyawoodpreservativeproductinitsreadytouseform 4Principl
22、e Thetestpreservativeisappliedbybrushingtothelongitudinalfacesofaseriesoftestspecimensofasusceptible woodspecies.Thetreatedtestspecimensareexposedtofeederblockscolonizedbypureculturesof basidiomycetesfungi.Thelateralpenetrationofthedifferentfungithroughtheexposedsurfaceofthetest specimensisassessedf
23、romsawncrosssectionsofthetestsamplesattheendoftheexposureperiod. 5 Testmaterialsandapparatus 5.1Biological material Thetestfungitobeusedareasfollows:CEN/TR14839:2004(E) 6 5.1.1 Obligatorytestfungi(brownrots) onScotspinesapwood. Coniophoraputeana (SchumacherexFries)Karsten(BAMEbw.15). Gloeophyllumtra
24、beum (PersoonexFries)Murrill(BAMEbw.109). Poriaplacent a(Fries)CookesensuJ.Eriksson(FPRL280). ThestrainsshallbeobtainedandmaintainedinaccordancewithannexB. 5.1.2 Obligatoryfungus(whiterot)onbeech,iftestsincludingawhiterotfungusarealsotobeundertaken. Coriolusversicolor (Linnaeus)Qulet(CTB863A). Thest
25、rainshallbeobtainedandmaintainedinaccordancewithannexB. 5.1.3 Additionalfungalspecies Ifadditionalfungiareused,adescriptionofthestrain(s)equivalenttothatoftheobligatoryfungigiveninannexB shallberecordedinthetestreport. Thestrain(s)shallbemaintainedinaccordancewiththeinstructionsfromtheirlaboratoryof
26、origin. 5.2 Feederblocks 5.2.1Wood species Scotspinesapwood(PinussylvestrisLinnaeus)shallbeusedforfeederblocksforbrownrotfungiandbeech (Fagussylvatica Linnaeus)forwhiterotfungi. 5.2.2Wood quality Thewoodshallbesoundandwithoutknots.Thewoodshallnothavebeenwaterstored,floated,chemically treatedorsteame
27、d.TheScotspineshallbeexclusivelysapwoodcontaininglittleresin. NOTE Woodthathasbeenkilndriedattemperaturesbelow60Cmaybeused. 5.2.3Dimensions Thedimensionsoffeederblocksmeasuredat12%( m/m)moisturecontentshallbe: (50 0,5)mm (25 0,5)mm (15 0,5)mm 5.2.4 Numberoffeederblocks Fourfeederblocksshallbeprepare
28、dforeachtestspecimenthatistobeused(see7.5). 5.3 Productsandreagents 5.3.1 Watercomplyingwithgrade3ofENISO3696. 5.3.2 Culturemediumforfeederblocks: maltextractcontaining(0,9 0,3)%( m/m)nitrogen: concentrated :50g; inpowderform :40g;CEN/TR14839:2004(E) 7 agarcontainingapproximately0,3%( m/m)nitrogenan
29、dcausingnoinhibitionofgrowthoffungi:20g; water:1000ml. Combinetheingredientsandheattodissolve.Dispenseintoeachculturevessel(5.4.5)asufficientquantitytogive adepthofbetween3mmand4mm.Closethevesselsandsterilizeintheautoclave(5.4.7)at121Cfor20min. Cooltoroomtemperaturewhilstlyingflat. NOTE Alternativel
30、y,themediumcanbesterilizedthendispensedintosterilevesselsunderasepticconditions. 5.3.3 Testsubstratefortestspecimens Anhydrated,laminar,aluminiumironmagnesiumsilicate 1) exfoliatedtoyieldparticlesupto3 mmdiameter. Particleslessthan1mmshallberemovedbysieving.Beforeuse,thoroughlymixthesampleoftestsubs
31、trate. Thetestsubstrateshallbeusedonlyonce. 5.3.4 Acidifyingsolutionforthetestsubstrate Anacidifyingsolutionshallbepreparedwiththefollowingcomposition: potassiumchloride(KCl)solution,0,1mol/l:950ml; hydrochloricacid(HCl),solution0,1mol/l:50ml. 5.3.5 Endsealingproduct Amaterialresistanttothepenetrati
32、onofthetestpreservativeandthetestfungiandwithoutanyfungistaticor fungicidalactivitywithinthetestspecimen. NOTE Threecoatsofa2componentepoxylacquerhavebeenfoundtobesuitable. 5.3.6 Fungicidalsolutionforassessment Prepareastocksolutionofthefollowingcomposition: Phenol 0,5g (methyl1(butylcarbamoyl)benzi
33、midazol2ylcarbamate):active ingredient(benomyl 2) ) 0,16g 2,6dichloro4nitroaniline 0,16g Ethanolsolution50%(V/V) 50ml Preparethereadyforusesolutionbyadding5mlofthestocksolutionto1lwater(see5.3.1). 5.3.7 Stainingsolution 3) Asolutionof0,04%( m/m)bromophenolblueinethanolsolution,50%(V/V). 1) Vermiculi
34、teissuitable. 2) Proprietyproductsnormallycontain50%(m/m)benomyl. 3) Optionallyasanaidtoassesspenetrationofmyceliumintothewoodafterexposure.CEN/TR14839:2004(E) 8 5.4Apparatus 5.4.1 Conditioningchamber ,wellventilatedandcontrolledat(20 2)Cand(65 5)%relativehumidity. 5.4.2 Culturechamber (incubatororr
35、oom),darkandcontrolledat(22 2)Cand(70 5)%relativehumidity. 5.4.3 Laboratoryworkarea ,wellventilated,wheretreatmentofthetestspecimensiscarriedout CAUTIONItisessentialtofollowsafetyproceduresforhandlingflammableandtoxicmaterials. 5.4.4 Testcontainers Madeofamaterialwhichdoesnothaveatoxiceffectonthetes
36、tfungi,andprovidedwithaventilatedlid.They shallhaveavolumeofbetween2land3l,andminimumdimensionsof(140 120)mmand85mmindepth. NOTE1 Thesedimensionsarenecessarytoallowaminimumof20mmbetweenthetwotestspecimensand10mm betweeneachtestspecimenandthecontainer. NOTE2 AsuitablecontainerisshowninFigure5. NOTE3
37、Itisconvenientifthecontainerscanbesterilizedbyautoclaving. 5.4.5 Culturevessels Withacapacityofbetween400mland650ml,providingaflatsurfaceareaofbetween90cm 2 and120cm 2 for themediumandprovidedwithaventilatedclosure. NOTE CulturevesselsusedinEN113aresuitable. 5.4.6 Ordinarylaboratoryequipmentincludin
38、g abalancecapableofweighingtothenearestof0,01g. 5.4.7 Autoclave Capableofbeingcontrolledandmaintainingatemperatureof121C. 5.4.8 Sawingequipment Finetoothedsawingmachineforcuttingoftestspecimensforevaluationattheendofthetest. 5.4.9 Testspecimensupports Madeofamaterialwhichhasnoeffectonthetestfungiand
39、doesnotreactwiththepreservative.Theyshallbeof anopentexture,haveathicknessof(3 0,5)mmandofasufficientareatosupportsecurelythetestspecimenon thefeederblocks. NOTE Polyethylenemeshhasbeenfoundtobesuitable. 5.4.10 Feederblocksupports Madeofamaterialwhichdoesnotreactwiththeculturemediumandhasnoeffectont
40、hetestfungi.Theyshallbe ofanopentexture,haveathicknessof(3 0,5)mmandofsufficientareatosupportsecurelythefeederblocks. NOTE Polyethylenemesh,glassrodsandstainlesssteelrodshavebeenfoundtobesuitable. 5.4.11 Dryingsupports, thatwillgiveaminimumcontactwiththetreatedtestspecimenstobeplacedonthem. Thesuppo
41、rtsshallbemadeofamaterialwhichdoesnotreactwiththetestsolventortestpreservative,for exampleglassfororganicproductsorplasticsmaterialforsaltscontainingfluorine. 5.4.12 Equipmentforchemicalgassterilizationoraccesstoaradiationservice(seeannexC).CEN/TR14839:2004(E) 9 5.4.13 Facilitiesforvacuumfiltration
42、Comprisingvacuumsource,filterflask,Bchnerfunnelandcoarsegradefittingfilterpapersof125mmdiameter. 6 Samplingofthepreservative Thesampleofpreservativeshallberepresentativeoftheproducttobetested.Samplesshallbestoredand handledinaccordancewithanywritteninstructionsfromthesupplier. NOTE Forthesamplingofp
43、reservativesfrombulksupplies,theproceduregiveninEN212shouldbeused. 7 Testspecimens 7.1 Speciesofwood ThetestshallbecarriedoutonScotspinesapwood( PinussylvestrisLinnaeus)forsoftwoodsandbeech( Fagus sylvaticaLinnaeus)forhardwoods. 7.2Wood quality Thewoodshallbefreefromcracks,stain,decay,insectdamageorotherdefects.Thewoodshallnothavebeen waterstored,floated,chemicallytreatedorstea
