1、BSI Standards PublicationDurability of wood and wood-based products Assessment of theeffectiveness of a masonry fungicide to prevent growth into wood of Dry Rot Serpulalacrymans (Schumacher ex Fries)S.F. Gray Laboratory methodPD CEN/TS 12404:2015National forewordThis Published Document is the UK imp
2、lementation of CEN/TS 12404:2015.It supersedes DD ENV 12404:1997 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication d
3、oes not purport to include all the necessary provisions ofa contract. Users are responsible for its correct application. The British Standards Institution 2015.Published by BSI Standards Limited 2015ISBN 978 0 580 86284 7ICS 71.100.50Compliance with a British Standard cannot confer immunity fromlega
4、l obligations.This Published Document was published under the authority of theStandards Policy and Strategy Committee on 31 March 2015. Amendments/corrigenda issued since publicationDate Text affectedPUBLISHED DOCUMENTPD CEN/TS 12404:2015TECHNICAL SPECIFICATION SPCIFICATION TECHNIQUE TECHNISCHE SPEZ
5、IFIKATION CEN/TS 12404 February 2015 ICS 71.100.50 Supersedes ENV 12404:1997English Version Durability of wood and wood-based products - Assessment of the effectiveness of a masonry fungicide to prevent growth into wood of Dry Rot Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Laboratory method
6、 Durabilit du bois et des matriaux drivs du bois - valuation de lefficacit dun fongicide de maonnerie pour empcher le dveloppement dans le bois de la mrule Serpula lacrymans (Schumacher ex Fries) S.F. Gray - Mthode de laboratoire Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der Wirksamkei
7、t eines Schutzmittels gegen das berwachsen von Echtem Hausschwamm Serpula lacrymans (Schumacher ex Fries) S.F. Gray vom Mauerwerk auf das Holz - Laboratoriumsverfahren This Technical Specification (CEN/TS) was approved by CEN on 6 October 2014 for provisional application. The period of validity of t
8、his CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard. CEN members are required to announce the existence of this CEN/TS in the same way
9、as for an EN and to make the CEN/TS available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached. CEN members are t
10、he national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Roman
11、ia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by
12、any means reserved worldwide for CEN national Members. Ref. No. CEN/TS 12404:2015 ECEN/TS 12404:2015 (E) 2 Contents Page Foreword 3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Principle 5 5 Test materials .6 5.1 Test fungus .6 5.1.1 Obligatory test fungus 6 5.1.2
13、Optional test fungi .6 5.1.3 Maintenance of strains 6 5.2 Products and reagents 6 5.2.1 Water, distilled or deionized, conform to grade 3 of EN ISO 3696. 6 5.2.2 Malt mineral salt agar culture medium; consisting:.6 5.3 Apparatus .7 5.3.1 Culture vessels 7 6 Sample of the preservative .8 7 Mortar tes
14、t specimen .9 7.1 Preparation of mortar 9 7.2 Preparation of mortar test specimen .9 7.3 Curing of mortar test specimen 11 7.4 Leaching of mortar test specimen 11 7.5 Number and distribution of mortar test specimens 11 8 Wood test specimens . 12 8.1 Species of wood 12 8.2 Quality of wood . 12 8.3 Pr
15、ovision of wood test specimens 12 8.4 Dimensions of wood test specimens 12 9 Procedure 12 9.1 Culturing the test fungus . 12 9.2 Treatment of mortar test specimens. 12 9.3 Preparation of wood test specimens 12 9.4 Sterilization procedures . 13 9.5 Exposure to fungus 13 9.6 Examination of the mortar
16、test specimens 13 9.7 Validity of test . 14 10 Statement of the results . 15 11 Test report . 15 Annex A (informative) Test fungi 16 Annex B (informative) Methods of sterilization . 18 Annex C (informative) Example of a test report 20 PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 3 Foreword This documen
17、t (CEN/TS 12404:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC s
18、hall not be held responsible for identifying any or all such patent rights. This document supersedes ENV 12404:1997. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to announce this Technical Specification: Austria, Belgium
19、, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerla
20、nd, Turkey and the United Kingdom. PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 4 Introduction This Technical Specification describes a laboratory method of test for the assessment of the effectiveness of a masonry fungicide applied to masonry for the prevention of the growth of dry rot, Serpula lacrym
21、ans (Schumacher ex Fries) S.F. Gray into wood. This laboratory method enables the determination of the concentration of a preservative within mortar which could prevent the dry rot fungus from growing through a given mortar layer treated with this preservative. PD CEN/TS 12404:2015CEN/TS 12404:2015
22、(E) 5 1 Scope This Technical Specification specifies a method for determining the performance of a preservative, applied to the upper surface of the mortar test specimens, in preventing the growth of dry rot through the treated mortar when exposed to the test fungus. This method is only applicable t
23、o masonry fungicides applied as a true solution of the preservative in water or dilute oil in water emulsion. It is not applicable to rods, pastes and other similar preservative types. This method is applicable to preservatives applied to masonry by brushing, spraying and/or injection techniques or
24、mixed into rendering and plastering mortar for masonry. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest
25、 edition of the referenced document (including any amendments) applies. EN 113:1996, Wood preservatives - Test method for determining the protective effectiveness against wood destroying basidiomycetes - Determination of the toxic values EN 413-1, Masonry cement - Part 1: Composition, specifications
26、 and conformity criteria EN 459-1, Building lime - Part 1: Definitions, specifications and conformity criteria EN 599-1, Durability of wood and wood-based products - Efficacy of preventive wood preservatives as determined by biological tests - Part 1: Specification according to use class EN ISO 3696
27、, Water for analytical laboratory use - Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 masonry fungicide fungicidal/fungistatic product applied to masonry and other mineral construction materials to
28、prevent the growth of dry rot through or over the treated material 3.2 performance behaviour of the preservative product in terms of its effectiveness in test 3.3 preservative formulated masonry fungicide in the form received from the supplier for the test 3.4 supplier sponsor of the test 4 Principl
29、e The preservative to be tested is applied by pipette (or in accordance with the sponsors instruction) to the upper surface of mortar test specimens. The mortar test specimens are contained in rigid tubes and an untreated wooden sample is placed on top of these mortar test specimens. The bases of th
30、e mortar specimens are exposed to dry rot attack for a given time. The assessment of the performance of the test PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 6 preservative consists of checking the growth of the fungus through the mortar and the evaluation of any attack of the wooden sample contained i
31、n the rigid tube. 5 Test materials 5.1 Test fungus 5.1.1 Obligatory test fungus Serpula lacrymans (Schumacher ex Fries) S.F. Gray, strain BAM Ebw.315. 5.1.2 Optional test fungi For specific regional uses or conditions, it is also possible to use other strains of dry rot (e.g.Serpula lacrymans FPRL 1
32、2 C) known to be capable of growing through masonry. NOTE Other fungal species can grow through masonry. This method of test could be used to assess the ability of these fungi to grow through mortar specimens. 5.1.3 Maintenance of strains The strains shall be maintained and treated in accordance wit
33、h the instructions from their laboratory of origin (see annex A). If a strain shows signs of degeneration, it shall no longer be used and the testing laboratory shall obtain a new standard culture of the strain. 5.2 Products and reagents 5.2.1 Water, distilled or deionized, conform to grade 3 of EN
34、ISO 3696. 5.2.2 Malt mineral salt agar culture medium; consisting: malt extract in concentrated form 12,50 g or in powder form 10,00 g agar causing no inhibition of growth of fungi 15,00 g potassium dihydrogen phosphate (KH2PO4) 2,72 g calcium sulfate dihydrate (CaSO4.2H2O) 0,38 g magnesium sulfate
35、heptahydrate (MgSO4.7H2O) 0,62 g water (5.2.1) to make up to 1 000 ml. Place all the ingredients in a 1 000 ml beaker measure and gently heat, stirring occasionally, until completely dissolved. Pour 150 ml of the culture medium into each culture vessel (5.3.1). Close the vessels with screw cap witho
36、ut a hole a quarter of a turn less than full closure and sterilise the closed vessels in the autoclave (5.3.8) at (121 2) C for 30 min. Let them cool standing upright. 5.2.3 Nutrient solution, a mass fraction for 5 % aqueous solution of malt extract. 5.2.4 Equipment for chemical gas or for steam ste
37、rilisation or access to a radiation source (see Annex B). 5.2.5 Carbon dioxide, compressed gas in cylinders. PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 7 5.2.6 Sodium chloride, saturated solution in water. 5.2.7 Portland cement, conforming to EN 413-1. 5.2.8 Hydrated building lime, conforming to EN 4
38、59-1. 5.2.9 Bricklaying mortar sand, quartz sand with a particle size equal to or less than 1 mm, washed under running tap water until the water is no longer turbid. 5.3 Apparatus 5.3.1 Culture vessels Straight sided flat bottom glass culture vessels with an aperture of 50 mm to 60 mm (see Figure 3)
39、, provided with both screw caps without a hole, used for culturing the test fungus (9.1), and screw caps with a central hole equal in size to the outer diameter of the tube (5.3.2.) plus the thickness of the tubing (5.3.3.) in diameter. NOTE The alternative type C.2 of test vessels described in EN 1
40、13, have been found to be suitable. 5.3.2 Rigid tubes, which can be sterilized using an autoclave (for example glass, or polyvinylidene fluoride) with an inner diameter of 35 mm to 46 mm and a length of at least 150 mm. 5.3.3 Tubing, with a diameter corresponding to the outer diameter of the rigid t
41、ubes (5.3.2.) with a wall thickness of (1,0 0,5) mm and cut into lengths of (40,0 1,0) mm capable of being sterilized using an autoclave. NOTE Tubing made of rubber has been found to be suitable. 5.3.4 Inert supports of maximum thickness 3 mm and when in use, do not obscure more than 10 % of the mor
42、tar surface. NOTE Stainless steel washers of overall diameter 25 mm have been found to be suitable. Two supports are required for each test assembly. 5.3.5 Conditioning chamber, well ventilated and controlled at (20 2) C and (65 5) % relative humidity. 5.3.6 Culture chamber, dark and controlled at (
43、22 1) C and (70 5) % relative humidity. 5.3.7 Drying oven, capable of being controlled at (45 1) C. 5.3.8 Autoclave, adjustable to (121 2) C. 5.3.9 Containers, to prepare the mortar and the preservative solutions, made of a material that does not react with their contents. 5.3.10 Mortar mould appara
44、tus, consisting of a plastic frame for example polyvinyl chloride (PVC) with a height of (10 0,5) mm, a porous support (for example clay house building bricks, ceramic plates) to absorb the excess water in the fresh mortar and a cloth (for example muslin cloth, cheese cloth) with the same dimensions
45、 as the frame to aid demoulding the mortar specimens (see Figure 2). 5.3.11 Plastic lath, used to smooth the surface of the mortar after casting in the plastic frame. 5.3.12 Circular tamper with a flat base, a diameter of 3 mm to 5 mm to less than the internal diameter of the rigid tube (5.3.2), and
46、 at least 50 mm longer than the rigid tube. 5.3.13 Racks on which to place the treated mortar test specimens an example is shown in Figure 1. PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 8 Dimensions in millimetres Key 1 wad of cotton wool 2 tubing 3 culture vessel screw cap 4 rigid tube 5 mortar speci
47、men Figure 1 Example of a rack 5.3.14 Sterile single-use pipettes of (1,0 0,1) ml content. 5.3.15 Ordinary laboratory equipment, including for example balances accurate to 0,01 g, sealable containers, forceps. 5.3.16 Microbiological safety cabinet, providing protection to the operator and to the wor
48、k. 6 Sample of the preservative The sample shall be representative of the product to be tested. It shall be identified as specified in EN 599-1. PD CEN/TS 12404:2015CEN/TS 12404:2015 (E) 9 7 Mortar test specimen 7.1 Preparation of mortar Dry a quantity of the quartz sand (5.2.9) in the drying oven (
49、5.3.7) at (45 1) C. Measure out nine parts by volume of the dried quartz sand and weigh, then place into a container (5.3.9). Add two parts by volume hydrated building lime (5.2.8) and one part by volume Portland cement (5.2.7) and mix thoroughly. Add 16 ml water (5.2.1) per 100 g quartz sand and mix until a homogeneous mortar is obtained. 7.2 Preparation of mortar test specimen Before casting the fresh mortar in the mortar mould apparatus (5.3.10), soak the porous support in water for 30 min. Place
