1、CGSB CAN/CGSB-4-2 N0=28.L-M9L tt 1874650 0011250 465 = CAN/CGSB-4.2 NO. 28.1-M91 NATIONAL STANDARD OF CANADA Textile Test Methods Resistance to Micro-organisms - Fungus Damage Test - Pure Culture - Quantitative Canadian General Standards Board Copyright Canadian General Standards Board Provided by I
2、HS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-The CANADIAN GENERAL STANDARDS BOARD (CGSB), under whose auspices this standard has been developed is a government agency within the Federal Department of Supply and Services. CGSB is engaged
3、 in the production of voluntary standards in a wide range of subject areas through the media of standards committees and the consensus process. The standards committees are composed of represeniatives of relevant interests including producers, consumers and other users, retailers, governments, educa
4、tional institutions, technical, professional and trade societies, and research and testing organizations. Any given standard is developed on the consensus of views expressed by such representatives. The Ministers Advisory Council on CGSB reviews the results of the consensus process. CGSB has been ac
5、credited by the Standards Council of Canada as a national standards-writing organization. The standards that it develops and offers as National Standards of Canada conform to the criteria and procedures established for this purpose by the Standards Council of Canada. In addition to standards it publ
6、ishes as national standards, CGSB produces standards to meet particular needs, in response to requests from a variety of sources in both the public and private sectors. Both CGSB standards and national standards developed by CGSB are developed in conformance with the policies described in the Policy
7、 Manual for the Development and Maintenance of Standards by CGSB. CGSB standards are subject to review and revision at any time, so as to ensure that they keep abreast of technological progress. Suggestions for their improvement, which are always welcome, should be brought to the notice of the stand
8、ards committees concerned. Changes to standards are issued either as separate amendment sheets or in new editions of standards. An up-to-date listing of CGSB standards, including details on latest issues and amendments, and ordering instructions, will be found in the CGSB Catalogue, which is publish
9、ed annually and is available without charge upon request. Although the intended primary application of this standard is stated in its Scope, it is important to note that it remains the responsibility of the users of the standard to judge its suitability for their particular purpose. Many tests requi
10、red by CGSB standards are inherently hazardous. CGSB neither assumes nor accepts any responsibility for any injury or damage that may occur during or as the result of tests, wherever performed. CGSB takes no position respecting the validity of any patent rights asserted with any item connected with
11、this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights are entirely their own responsibility. Further information on CGSB and its services and standards may be obtained from: The Secretary Canadian General Standards Board Ottawa, Cana
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16、da indicates that a standard conforms to the criteria and procedures established by the Standards Council of Canada. Approval does not refer to the technical content of the standard; this remains the continuing responsibility of the accredited standards-writing organization. Those who have a need to
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18、anada rests with the: Standards Council of Canada 350 Sparks Street Ottawa, Ontario KIP 6N7 Iow to order Publications: by Telephone - (819) 956-0425 or - (819) 956-0426 by Fax - (819) 956-5644 by Mail - CGSB, Sales Unit Ottawa, Canada KIA 1G6 in Person - Place du Portage III, 9C1 11 Laurier Street H
19、ull, Quebec Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-CGSB CAN/CGSB-4-2 N0.28-1-fl91 * 1874650 0011252 238 = NATIONAL STANDARD OF CANADA CANICGSB-4.2 NO. 28.1-M91 TEXTILE TEST M
20、ETHODS RESISTANCE TO MICRO-ORGANISMS - FUNGUS DAMAGE TEST - PURE CULTURE - QUANTITATIVE This method supersedes issue of July 1977. Prepared by the Canadian General Standards Board sa Standards Council of Canada Approved by the Published August 1991 by the Canadian General Standards Board Ottawa, Can
21、ada KIA 1G6 Minister of Supply and Services Canada - 1991 No part of this publication may be reproduced in any form without the prior permission of the publisher. Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted w
22、ithout license from IHS-,-,-CANADIAN GENERAL STANDARDS BOARD COMMITTEE ON TEXTILE TEST METHODS (Membership at date of approval) Audet, A.J. Chairman Biron, C. Clarke, N. Crown, E.M. Demers, A. Dolhan, P. Edwards, D.S. Graham, M. Hurley, M. Kasem, M.A. King, M. Knapp, C. Mitton, M.T. Monk, W.B. Morti
23、mer, R. Muniak, E. Patel, A. Rashid, M.A. Routhier, D. Sanders, B.A. Scrafton, K. White, J. Wishart, P. Randall, J. Secretary Dominion Textile Inc. Celanese Canada Inc. Du Pont Canada Inc. University of Alberta Professional Association of Cleaners and Launderers of Quebec Department of National Defe
24、nce Canadian Carpet Institute The Wool Bureau of Canada Ltd. Department of National Revenue (Customs and Excise) Department of Consumer and Corporate Affairs University of Manitoba Department of Consumer and Corporate Affairs Consultant Consultant ORTECH International The T. Eaton Company Canada Ltd
25、. Sears Canada Inc. Department of Public Works, Alberta Consoltex Inc. Department of Supply and Services The Retail Research Foundation of Canada Patons & Baldwins (Canada) Inc. Technitrol Canada Ltd. Canadian General Standards Board Acknowledgment is made for rhe French translation of this National
26、 Standard of Canada by the Translation Bureau of the Department of the Secretary of State. CANKGSB-4.2 NO. 28.1-M91 Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-5 Ottawa Canada K1A
27、 1G6 1. 1.1 1.2 1.3 2. 2.1 3. 3.1 3.1.1 3.2 4. 4.1 TEXTILE TEST METHODS CANICGSB-4.2 Resistance to Micro-organisms - Fungus Quantitative Damage Test - Pure Culture - NO. 28.1-M91 Supersedes Issue of July 1977 PURPOSE AND SCOPE This method determines the resistance of textile materials containing cel
28、lulosic fibres to deterioration by the action of a cellulolytic fungus. The method is of particular importance in testing fabrics designed for outdoor use. The test is of moderate severity and is not recommended for treated fabrics intended for use under conditions involving contact with soil. (See
29、CAN/CGSB-4.2 No. 28.3-M.) The testing and evaluation of a product against this method may require the use of materials and/or equipment that could be hazardous. This document does not purport to address all the safety aspects associated with its use. Anyone using this method has the responsibility t
30、o consult the appropriate authorities and to establish appropriate health and safety practices in conjunction with any existing applicable regulatory requirements prior to its use. PRINCIPLE The fabric is leached with water to remove any fungicidal substance likely to be rapidly removed by wetting,
31、and to ensure that all specimens be in uniform condition with respect to shrinkage. Specimens taken from the leached fabric are subjected to the action of a cellulolytic fungus under controlled conditions, and in the absence of any source of assimilable carbon other than that present in the test mat
32、erial. The resistance of the fabric is judged by the loss in strength resulting from exposure to the organism for a period of time known to cause severe loss of strength in cellulosic materials that have not received any protective treatment. APPLICABLE PUBLICATIONS The following publications are ap
33、plicable to this method: Canadian General Standards Board (CGSB) CAN/CGSB-4.2 - Textile Test Methods: No. 2-M - Conditioning Textile Materials for Testing No. 9.1-M - Breaking Strength of Fabrics - Strip method (Constant-time-to-break Principle) No. 9.4-M - Breaking Strength of Yarns - Single Strand
34、 Method (Constant-time-to-break Principle) No. 28.3-M - Resistance to Micro-organisms - Soil Burial Test. Reference to the above publications is to the latest issues, unless otherwise specified by the authority applying this method. The source for these publications is shown in the Notes section. AP
35、PARATUS AND MATERIALS Organism - Chaefomium Globosum, ATCC No. 6205 (Note 1): grow on sterile filter paper placed on mineral salts agar described in par. 4.2. Note 1: Cultures of this organism may be obtained from American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD 20852, U.S.A. 1
36、Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-4.2 4.3 4.4 4.5 4.6 4.7 4.7.1 4.7.2 4.7.3 4.8 5. 5.1 5.2 2 CGSB CAN/CGSB-4.2 N0.28.1-ML * D 1874650 0011255 T47 m Maintenance of organi
37、sm: the fungus is grown on agar slants in test tubes, and spore suspensions are made in sterile distilled water as required for this test. Subcultures should be made on filter paper placed on mineral salts agar at sufficiently frequent intervals to maintain viable pure cultures. Cultures, under refr
38、igeration, may be kept as long as six to nine months. Salts - Agar culture medium: a typical satisfactory formula is: Sodium nitrate (NaN03) 2.0 g Magnesium sulphate (MgS04) 0.5 g Potassium chloride (KCl) 0.5 g Ferric sulphate (Fez( SO&- H20) Potassium orthophosphate monohydrogen (K2“04) Yeast extra
39、ct 0.02 g 0.01 g 1.2 g Potassium orthophosphate dihydrogen (KH2P04) 0.14 g Agar 15.0 g Distilled Water 1.0 L Adjust pH to 7.2 f 0.1 with hydrochloric acid, plus sodium hydroxide if necessary, and store in flask. Autoclave: an autoclave capable of being operated at a steam pressure of 103 to 117 kPa
40、(gauge) and an exhaust temperature of 121 to 123OC. these being the sterilizing conditions used throughout. Incubator: an incubator room or cabinet maintained at 30 f 2C. Petri dishes: 150 mm in diameter. Leaching Apparatus This shall consist of a reservoir of water at 24 f 3OC from which water is p
41、assed to a suitable vessel containing the fabric to be leached. The size of this vessel will depend on the size of the sample to be leached. The rate of flow of water through the vessel should be such as to provide approximately four changes of water per hour. The water used must not contain any mat
42、erial that may react with any of the constituents of the sample being tested (Note 2). Wherever possible, distilled or demineralized water shall be used. The pH of the water shall not be less than 5.0 nor greater than 8.0. Low power binocular microscope (SOX). TEST SPECIMENS Fabrics - A portion appr
43、oximately 200 x 400 mm, with the shorter dimension parallel to the warp, shall be cut from the test sample and leached for 24 h (Note 3). Nine warpwise strips, each approximately 200 x 35 mm, shall be cut from the leached portion, ravelled to a width of exactly 25 mm, and cut into two warpwise strip
44、s each 100 mm long. The nine 100 mm long specimens from the upper half of the 200 x 400 mm piece shall be kept together in one lot and the nine specimens from the lower half in Note 2: An example of the use of an unsuitable type of water is to employ a water containing alkaline impurities to leach a
45、 sample carrying a phenolic type fungicide. Depending on the degree of alkulinity, solubilization of the fungicide y the alkali in the water will occur through the formation of a soluble phenate, and the loss thus occasioned would not represent a loss through water (pH 6-8) solubility of the origina
46、l phenolic compound. Note 3: To facilitate wetting by the spore suspension, it is desirable that the inoculation of the specimens (section 6) be proceeded with immediately following leaching, before the specimens have become dry. Where drying has occurred, the specimens should be dampened by being t
47、horoughly sprayed with distilled water and allowed to stand for 1 to 2 h,flat, on a non- absorbent sulface in a closed container, to avoid dessication. NO. 28.1-M91 Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted
48、 without license from IHS-,-,-5.3 5.4 5.5 6. 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 6.10 6.1 1 6.12 7. 7.1 7.2 another. In this way each specimen in one lot contains the same warp yams as a corresponding specimen in the other lot. One lot of nine 100 mm ravelled strips are used as control specimens, th
49、e other lot of nine being subjected to the action of the organism. Yarns - Forty-two 500 mm lengths shall be taken from the sample and wound into loops approximately 50 mm long, and leached for 24 h (Note 3). Twenty-one loops are used as control specimens, the other twenty-one being subjected to the action of the organism. PROCEDURE Sterilize the required number of Petri dishes under the conditions specified in par. 4.4. Sterilize the salts-agar culture medium, pour i
