1、August 2015 Translation by DIN-Sprachendienst.English price group 15No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS
2、 13.060.70; 65.150!%E4“2342917www.din.deDDIN EN 14757Water quality Sampling of fish with multi-mesh gillnets;English version EN 14757:2015,English translation of DIN EN 14757:2015-08Wasserbeschaffenheit Probenahme von Fischen mittels Multi-Maschen-Kiemennetzen;Englische Fassung EN 14757:2015,Englisc
3、he bersetzung von DIN EN 14757:2015-08Qualit de leau chantillonnage des poissons laide de filets maillants;Version anglaise EN 14757:2015,Traduction anglaise de DIN EN 14757:2015-08SupersedesDIN EN 14757:2005-11www.beuth.deDocument comprises 33 pagesIn case of doubt, the German-language original sha
4、ll be considered authoritative.07.15DIN EN 14757:2015-08 2 A comma is used as the decimal marker. National foreword This document (EN 14757:2015) has been prepared by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation
5、was the DIN-Normenausschuss Wasserwesen (DIN Standards Committee Water Practice), Working Committee NA 119-01-03-05 UA Biologische Verfahren of NA 119-01-03 AA Wasseruntersuchung. Designation of the method: Sampling of fish with multi-mesh gillnets: Method DIN EN 14757 M 21 This standard has been pu
6、blished to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy*). Expert assistance and specialized laboratories will be required to perform the
7、 analyses described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent additional conditions will have to be specified. This standard has been prepared by the DI
8、N-Normenausschuss Wasserwesen (DIN Standards Committee Water Practice) in collaboration with the Wasserchemische Gesellschaft - Fachgruppe in der Gesellschaft Deutscher Chemiker (Water Chemistry Society - Division of the German Chemical Society). It is part of the series Deutsche Einheitsverfahren z
9、ur Wasser-, Abwasser- und Schlammuntersuchung (German standard methods for the examination of water, waste water and sludge): Sampling of fish with multi-mesh gillnets (M 21). Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes
10、. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Beuth Verlag GmbH and Wiley-VCH Verlag GmbH b) the sampling design for the location of benthic gillnets has been revised;
11、c) the requirements for the planning, sampling duration and sampling procedure have been revised; d) the requirements for data collection, data storage and data processing have been revised; e) the specifications concerning the handling of effects caused by gillnet selectivity have been revised and
12、shortened; f) details and references for alternative sampling methods have been included; g) details for age and growth analyses have been excluded from the normative part and added in an informative annex (Annex B); h) the standard has been technically and editorially revised. Previous editions DIN
13、 EN 14757: 2005-11 DIN EN 14757:2015-08 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 14757 May 2015 ICS 13.060.70; 65.150 Supersedes EN 14757:2005English Version Water quality - Sampling of fish with multi-mesh gillnets Qualit de leau - Echantillonnage des
14、poissons laide de filets maillants Wasserbeschaffenheit - Probenahme von Fischen mittels Multi-Maschen-Kiemennetzen This European Standard was approved by CEN on 16 April 2015. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this Eu
15、ropean Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versi
16、ons (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria,
17、Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Sw
18、itzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN nati
19、onal Members. Ref. No. EN 14757:2015 EEN 14757:2015 (E) 2 Contents Page Foreword 4 Introduction .5 1 Scope 6 2 Normative references 6 3 Terms and definitions .6 4 Principle 6 5 Equipment 6 5.1 Benthic gillnets 6 5.2 Pelagic gillnets .7 6 Sampling design and procedure 7 6.1 Sampling design 7 6.2 Plan
20、ning 8 6.3 Sampling period .8 6.4 Sampling .9 6.5 Safety instructions.9 7 Time series sampling 9 7.1 Sampling effort .9 7.2 Depth stratification of benthic gillnets . 10 7.3 Sampling of the pelagic habitat . 12 8 Inventory sampling . 12 8.1 Sampling effort 12 8.2 Depth stratification of benthic gill
21、nets . 12 9 Data handling and reporting 13 9.1 Fish data 13 9.2 Supplementary data . 14 9.3 Databases and quality control . 15 10 Dealing with gillnet selectivity . 16 11 Estimation of sampling variance . 16 11.1 Within-lake variation . 16 11.2 Within-lake and between-year variation . 17 11.3 Betwee
22、n-lake variation . 17 12 Applications and further analyses 17 13 Limitations and supplementary sampling 18 14 Alternative sampling 18 Annex A (informative) Distribution of benthic multi-mesh gillnets at different depth strata in lakes with different area and maximum depth . 19 Annex B (informative)
23、Sampling fish for age and growth analysis 21 B.1 General . 21 B.2 Selection of individuals 21 B.3 Choice of hard structure for age and growth analysis . 22 B.3.1 General requirements . 22 DIN EN 14757:2015-08 EN 14757:2015 (E) 3 B.3.2 Otoliths . 22 B.3.3 Scales . 23 B.3.4 Operculum bones 23 B.3.5 Cl
24、eithrum and metapterygoid 23 Annex C (informative) Example of forms for registration of fish and supplementary data . 24 Bibliography 27 DIN EN 14757:2015-08 EN 14757:2015 (E) 4 Foreword This document (EN 14757:2015) has been prepared by Technical Committee CEN/TC 230 “Water analysis”, the secretari
25、at of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2015, and conflicting national standards shall be withdrawn at the latest by November 2015. Attention is drawn
26、to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 14757:2005. This document contains the following technical changes compared w
27、ith the previous edition: a) this European Standard was revised to clarify that using pelagic gillnets is an option for fish sampling with gillnets; b) the sampling design for the location of benthic gillnets was revised; c) the requirements for the planning, sampling duration and sampling procedure
28、 were revised; d) the requirements for data collection, data storage and data processing were revised; e) the specifications concerning the handling of effects caused by gillnet selectivity were revised and shortened; f) details and references for alternative sampling methods were included; g) detai
29、ls for age and growth analyses were excluded from the normative part and added in an informative annex (Annex B). According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgar
30、ia, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turk
31、ey and the United Kingdom. DIN EN 14757:2015-08 EN 14757:2015 (E) 5 Introduction This is one of several European Standards developed for evaluation of species composition, abundance and age structure of fish in rivers, lakes and transitional waters. Other standards describe “Sampling of fish with el
32、ectricity” (EN 14011), “Guidance on the scope and selection of fish sampling methods” (EN 14962) and “Guidance on the estimation of fish abundance with mobile hydroacoustic methods” (EN 15910). In most countries the use of the method specified in this European Standard requires permits from landowne
33、rs and national or regional authorities. In many countries permits are also required from authorities for animal rights and animal welfare demands. Both fish diseases and diseases specific for other organisms, such as freshwater crayfish, may be spread by placing equipment contaminated with pathogen
34、s or parasites in the lake. The user of this method should check which national legislation is applicable. WARNING Persons using this European Standard should be familiar with usual laboratory and fieldwork practice. This European Standard does not purport to address all of the safety problems, if a
35、ny, associated with its use. It is the responsibility of the user to establish appropriate health and safety practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to this European Standard be carried out by sui
36、tably trained staff. DIN EN 14757:2015-08 EN 14757:2015 (E) 6 1 Scope This European Standard specifies a method for the sampling of fish in lakes, using benthic multi-mesh gillnets and gives recommendations on sampling of fish with pelagic multi-mesh gillnets. The method provides a whole-lake estima
37、te for species occurrence, quantitative relative fish abundance, biomass expressed as Catch Per Unit Effort (CPUE) and size structure of fish assemblages in temperate lakes. It also provides estimates that are comparable over time within a lake and between lakes. This European Standard specifies rou
38、tines for sampling, data handling and reporting, and provides information on applications and further treatment of data. It also provides guidance for the sampling of fish for age and growth analyses. According to the principles of this standard other lentic water bodies can be sampled. 2 Normative
39、references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) appli
40、es. EN 14962:2006, Water quality - Guidance on the scope and selection of fish sampling methods. 3 Terms and definitions For the purposes of this document, the terms and definitions given in EN 14962:2006 and the following apply. 3.1 sampling effort number of gillnet-nights Note 1 to entry: A gillne
41、t-night is one gillnet fishing during one night. For example a sampling effort of 8 gillnet-nights is 8 gillnets fishing during one night, or 4 gillnets fishing during 2 nights etc. 4 Principle The sampling procedure is based on stratified random sampling. The sampled lake is divided in depth strata
42、 and random sampling is performed within each depth stratum. Sampling of benthic fish is performed with specially designed multi-mesh gillnets which are 30 m long and 1,5 m deep. The gillnets are composed of 12 different mesh-sizes ranging from 5 mm to 55 mm knot to knot following a geometric series
43、. Similar nets can be applied also for sampling of pelagic fish. In larger and deeper lakes and reservoirs sampling of pelagic fish with multi-mesh gillnets is highly recommended. The sampling effort (number of gillnet-nights) necessary to allow detection of 50 % changes in relative abundance betwee
44、n sampling occasions, ranges between 8 gillnet-nights for small, shallow lakes, up to 64 gillnet-nights for lakes of about 5 000 ha. If less accurate estimates of abundance are needed, an inventory sampling procedure may be used, thereby reducing the necessary sampling effort. 5 Equipment 5.1 Benthi
45、c gillnets The multi-mesh gillnets have been designed for catching all types of freshwater fish species. Each gillnet shall be composed of 12 different mesh-sizes ranging from 5 mm to 55 mm (knot to knot). The mesh-sizes follow a geometric series, with a ratio between mesh-sizes of about 1,25. All g
46、illnets shall have the same order of mesh panels (see Table 1). DIN EN 14757:2015-08 EN 14757:2015 (E) 7 If experience has shown that large fish of certain species (e. g. bream Abramis brama, carp Cyprinus carpio, pikeperch Sander lucioperca, pike Esox lucius, tench Tinca tinca) are difficult to cat
47、ch with the mesh sizes shown in Table 1, it is possible to add larger mesh sizes. However, this modification shall be recorded in the report (fishing protocol) and the catch in the standardized mesh sizes shall be recorded separately from the catch in the added mesh sizes to allow comparisons betwee
48、n lakes and years. Table 1 Mesh-size distribution (knot to knot) and thread diameter in multi-mesh benthic gillnets Mesh no. Mesh size Thread diameter mm mm 1 43 0,20 2 19,5 0,15 3 6,25 0,10 4 10 0,12 5 55 0,25 6 8 0,10 7 12,5 0,12 8 24 0,17 9 15,5 0,15 10 5 0,10 11 35 0,20 12 29 0,17 Gillnets shall
49、 be made out of homogeneous, uncoloured nylon. Each gillnet shall be 30 m long and 1,5 m deep. Each mesh panel shall be 2,5 m long and mounted on a 30 m long buoyancy line (with a recommended linear density in water of 6 g/m), and a 33 m long lead line (recommended linear density in air 22 g/m and in water 9,9 g/m) made out of plastic in light grey colour. The diameter of the thread varies between 0,10 mm for the 5 mm mesh, to 0,25 mm for the 55 mm mesh (see Table 1). The hanging ratio is 0,5 for all mesh sizes. NOTE All mesh panels are commercially ava
