1、November 2014 Translation by DIN-Sprachendienst.English price group 9No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 87.040!%)F“2250635www.din.deDDIN EN 15457Paints and varnishes Laboratory method for testing the efficacy of film preservatives in acoating against fungi;English version EN 15457:2014,English translation of DIN EN 15457:2014-11Beschichtungsstoffe Laborverfahren fr die Prfung der Wirksamkeit von Fil
3、mkonservierungsmitteln in einerBeschichtung gegen Pilze;Englische Fassung EN 15457:2014,Englische bersetzung von DIN EN 15457:2014-11Peintures et vernis Mthode dessai en laboratoire permettant de dterminer lefficacit des prservateurs dufeuil dun revtement contre les champignons;Version anglaise EN 1
4、5457:2014,Traduction anglaise de DIN EN 15457:2014-11SupersedesDIN EN 15457:2007-10www.beuth.deDocument comprises 14 pagesIn case of doubt, the German-language original shall be considered authoritative.10.14 DIN EN 15457:2014-11 2 A comma is used as the decimal marker. National foreword This docume
5、nt (EN 15457:2014) has been prepared by Technical Committee CEN/TC 139 “Paints and varnishes” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the DIN-Normenausschuss Beschichtungsstoffe und Beschichtungen (DIN Standards Committee Coatings and Coating Material
6、s), Working Committee NA 002-00-15 AA Bautenbeschichtungen. Amendments This standard differs from DIN EN 15457:2007-10 as follows: a) the scope has been rendered more precise; b) a new term “active substance“ has been introduced (3.1); c) Annex B “Designation of the percentage area of disfigurements
7、 according to EN 16492:2014, Table A.3“ has been added; d) the standard has been editorially revised. Previous editions DIN EN 15457: 2007-10 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 15457 August 2014 ICS 87.040 Supersedes EN 15457:2007English Version Paints and varnishes - Laboratory me
8、thod for testing the efficacy of film preservatives in a coating against fungi Peintures et vernis - Mthode dessai en laboratoire permettant de dterminer lefficacit des prservateurs du feuil dun revtement contre les champignons Beschichtungsstoffe - Laborverfahren fr die Prfung der Wirksamkeit von F
9、ilmkonservierungsmitteln in einer Beschichtung gegen Pilze This European Standard was approved by CEN on 10 July 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without
10、any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other lang
11、uage made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmar
12、k, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE F
13、OR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15457:2014 E2 Contents Page Fore
14、word 3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Principle 5 5 Apparatus and materials .6 6 Fungi .6 6.1 Fungi more likely to grow in an exterior environment .6 6.2 Fungi more likely to grow in an interior environment7 7 Sampling and preparation of test samples
15、and of specimens .7 7.1 Sampling .7 7.2 Preparation of test samples (see Annex A) 7 7.3 Conditioning of the test samples .7 7.4 Preparation and number of specimens .7 8 Procedure .8 8.1 Preparation of the Petri dishes with the culture medium 8 8.2 Preparation of stock cultures and sub-cultures .8 8.
16、3 Preparation of the spore suspension 8 8.4 Inoculation and incubation (see Annex A) 8 8.5 Assessment 8 9 Test report 9 Annex A (informative) Laboratory method for testing the efficacy of film preservatives in a coating against fungi 10 Annex B (informative) Designation of the percentage area of dis
17、figurements according to EN 16492:2014, Table A.3 . 11 Bibliography . 12 DIN EN 15457:2014-11 EN 15457:2014 (E) 3 Foreword This document (EN 15457:2014) has been prepared by Technical Committee CEN/TC 139 “Paints and varnishes”, the secretariat of which is held by DIN. This European Standard shall b
18、e given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2015 and conflicting national standards shall be withdrawn at the latest by February 2015. Attention is drawn to the possibility that some of the elements of this docume
19、nt may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 15457:2007. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound
20、 to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
21、 Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. DIN EN 15457:2014-11 EN 15457:2014 (E) 4 Introduction This document identifies criteria to assess efficacy of film preservatives in a coating against fungi. The results of the method allow evaluation of an activ
22、e substance with regard to its inclusion in Annex I of the Biocidal Products Directive 98/8/EC (Directive 98/8/EC of the European Parliament and of the Council of 16 February 1998 concerning the placing of biocidal products on the market BPD) or in the list of the Biocidal Product Regulation (BPR, R
23、egulation (EU) 528/2012). The characteristics of the biocide treated coating material should conform to national regulations with regard to health, safety and the environment. DIN EN 15457:2014-11 EN 15457:2014 (E) 5 1 Scope This European Standard specifies a laboratory test method for determining t
24、he biocidal/biostatic efficacy of single active substances or combinations thereof used in film preservatives in a coating against fungal growth. This standard does not apply to coatings not susceptible to fungal growth. The test method comprises only active substances for film preservation, not the
25、 protection of the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for active substances used for wood and masonry coatings. It is not applicable to marine coatings. Safety, health and environmental aspects are not in the scope of this standard. De
26、termination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this standard. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dat
27、ed references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 12469, Biotechnology - Performance criteria for microbiological safety cabinets EN 16492:2014, Paints and varnishes - Evaluation of the surface
28、disfigurement caused by fungi and algae on coatings EN 23270, Paints and varnishes and their raw materials - Temperatures and humidities for conditioning and testing (ISO 3270) EN ISO 1513, Paints and varnishes - Examination and preparation of test samples (ISO 1513) 3 Terms and definitions For the
29、purposes of this document, the following term and definition applies. 3.1 active substance substance or micro-organism that has an action on or against harmful organisms SOURCE: Biocidal Product Regulation (BPR, Regulation (EU) 528/2012), Article 3.1 (c), modified the article “a“ between “or“ and “m
30、icro-organism“ was deleted 4 Principle To determine the fungicidal efficacy of film preservatives in a coating, the coating material is applied to a substrate conditioned according to EN 23270, placed onto an agar surface, inoculated with a standard fungal spore suspension and incubated. Conclusions
31、 can be drawn to the fungicidal efficacy of the film preservatives in a coating from the intensity of the fungal growth on the surface of the specimen after incubation. The method described here is a semiquantitative, comparative method between coatings, with and without film preservatives. DIN EN 1
32、5457:2014-11 EN 15457:2014 (E) 6 5 Apparatus and materials 5.1 Cutting device for preparing the specimens (coated filter paper with a diameter of 55 mm). 5.2 Autoclave 5.3 Incubator capable of maintaining (24 2) C. 5.4 Pipette, in the range between 100 l to 1 000 l, with sterile tips or combi-tips o
33、f 12,5 ml. 5.5 Filter paper without fungicidal effect (e.g. cellulose with a pore size of 0,45 m and a thickness of 650 m). 5.6 Automatic welding apparatus to seal the bags. 5.7 Sterilized glass bottles (100 ml). 5.8 Laboratory balance, capable of weighing to an accuracy of 0,1 g. 5.9 Microscope 5.1
34、0 Device to determine cell count (commercially available counting chamber, e.g. Thoma chamber). 5.11 Wetting agent (e.g. N-Methyltaurine). 5.12 Device for applying the coating 5.13 Sterilized test tubes or other sterilized glassware for preparing slant agar cultures. 5.14 Sterile Drigalski spatula 5
35、.15 Sterile platinum loop 5.16 Sterile glass funnel with cotton wool 5.17 Sterile Petri dishes (with a diameter of 94 mm, and a height of 16 mm). 5.18 Sterile tweezers 5.19 Sterile water 5.20 Class 2 microbiological safety cabinet according to EN 12469. 6 Fungi 6.1 Fungi more likely to grow in an ex
36、terior environment a) Aureobasidium pullulans (DSM1)2404) b) Alternaria alternata (DSM 62010) 1)DSM = DSMZ = Deutsche Sammlung fr Mikroorganismen und Zellkulturen (German collection of micro organisms and cell cultures), Braunschweig, Germany. DIN EN 15457:2014-11 EN 15457:2014 (E) 7 c) Cladosporium
37、 cladosporioides (DSM 62121) d) Phoma violaceae (IMI2)49948ii) e) Ulocladium atrum (IMI 79906 or DSMZ 63068) 6.2 Fungi more likely to grow in an interior environment a) Aspergillus versicolor (DSM 1943) b) Aspergillus niger (DSM 12634) c) Stachybotrys chartarum (DSMZ 2144) d) Penicillium purpurogenu
38、m (DSM 62866) e) Rhodotorula mucilaginosa (DSM 70825) The spore suspension used for the test shall be a mixture containing two fungi selected from the first group (6.1) and two fungi selected from the second group (6.2). 7 Sampling and preparation of test samples and of specimens 7.1 Sampling Take a
39、 representative sample of the coating material or of the coating system for testing in accordance with EN ISO 1513. 7.2 Preparation of test samples (see Annex A) Coat a strip of filter paper without biocidal effect with the coating material to be tested. The application rate shall be that recommende
40、d by the coating manufacturer for normal use. 7.3 Conditioning of the test samples Condition the test sample in a horizontal position for at least 5 days at (23 2) C and (50 5) % relative humidity, in accordance with EN 23270. NOTE The conditioning time might vary according to the coating material a
41、nd end use corresponding to information given by the manufacturer. 7.4 Preparation and number of specimens After conditioning, three specimens each with a diameter of 55 mm shall be prepared from the test samples. The specimens shall be sealed in a plastics bag and sterilized using gamma radiation o
42、f 10 kGy. Other methods of sterilization may be agreed between the parties. For each test series three specimens coated with coating material containing the film preservative, three specimens coated with the same coating material without film preservative and three specimens of the uncoated substrat
43、e shall be tested. 2)IMI = CABI = Bioscience Genetic Resource Collection, Egham, UK. DIN EN 15457:2014-11 EN 15457:2014 (E) 8 8 Procedure 8.1 Preparation of the Petri dishes with the culture medium A malt (3 %)-agar(1,5 %)-medium shall be sterilized in the autoclave. After cooling the medium to 55 C
44、 to 60 C, 20 ml shall be poured into each sterile Petri dish under aseptic conditions. 8.2 Preparation of stock cultures and sub-cultures Sub-cultures shall be obtained by inoculating spore material from a stock culture to freshly prepared agar slope culture media and shall be used for preparing the
45、 spore suspension. From these sub-cultures further sub-cultures can be derived in sufficient number. After the inoculation the sub-cultures shall be incubated at (24 2) C until good sporulation has been achieved. This might require 3 days to 7 days, depending on the fungal species used for testing.
46、The sub-cultures can be stored satisfactorily at 3 C to 7 C for a period of 3 months. 8.3 Preparation of the spore suspension For preparing the spore suspension a well-sporulating sub-culture is used. To this sub-culture 5 ml of sterile deionized water should be added (if required also add a surfact
47、ant e.g. 0,1 % N-Methyltaurine). The spores shall be carefully washed down from the agar slope, using a platinum loop as an aid, filtered through a sterile glass funnel with cotton wool and collected in a sterile glass bottle. After counting the spore concentration by using a commercially available
48、counting chamber and diluting each with sterile water to 106to 107spores/ml all spore suspensions intended for testing shall be mixed in equal parts. 8.4 Inoculation and incubation (see Annex A) In addition to the coated and uncoated specimens (see 7.4) a further three Petri dishes containing the nutritive agar medium only shall be inoculated. The sterilized specimens shall be placed centrally onto the surface of the culture media using sterile tweezers. The coated surface of the specimen shall be face up and
