1、January 2015 Translation by DIN-Sprachendienst.English price group 14No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 71.100.50!%?DM“2283342www.din.deDDIN EN 839Wood preservatives Determination of the protective effectiveness against wood destroyingbasidiomycetes Application by surface treatment;English version EN 839:2014,English translation of DIN EN 839:2015-01Holzschutzmittel Bestimmung der vorbeugenden Wirks
3、amkeit gegen Holz zerstrende Basidiomyceten Anwendung mit Oberflchenverfahren;Englische Fassung EN 839:2014,Englische bersetzung von DIN EN 839:2015-01Produits de prservation du bois Dtermination de lefficacit protectrice vis-vis des champignons basidiomycteslignivores Application par traitement de
4、surface;Version anglaise EN 839:2014,Traduction anglaise de DIN EN 839:2015-01SupersedesDIN CEN/TS 839:2008-06www.beuth.deDocument comprises 31 pagesIn case of doubt, the German-language original shall be considered authoritative.12.14DIN EN 839:2015-01 2 A comma is used as the decimal marker. Natio
5、nal foreword This document (EN 839:2014) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products” (Secretariat: AFNOR, France). The responsible German body involved in its preparation was the DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee
6、Timber and Furniture), Working Committee NA 042-03-06 AA Spiegelausschuss zu CEN/TC 38 und ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodukten. Amendments This standard differs from DIN CEN/TS 839:2008-06 as follows: a) the CEN/TS status has been changed to that of an EN Standard. Previous ed
7、itions DIN V ENV 839: 1994-06, 2002-06 DIN CEN/TS 839: 2008-06 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 839 November 2014 ICS 71.100.50 Supersedes CEN/TS 839:2008English Version Wood preservatives - Determination of the protective effectiveness against wood destroying basidiomycetes - Ap
8、plication by surface treatment Produits de prservation du bois - Dtermination de lefficacit protectrice vis-vis des champignons basidiomyctes lignivores - Application par traitement de surface Holzschutzmittel - Bestimmung der vorbeugenden Wirksamkeit gegen Holz zerstrende Basidiomyceten - Anwendung
9、 mit Oberflchenverfahren This European Standard was approved by CEN on 30 August 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists
10、 and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under t
11、he responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugo
12、slav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN
13、 DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 839:2014 EEN 839:2014 (E) 2 Contents Page Foreword 4 Introductio
14、n .5 1 Scope 6 2 Normative references 6 3 Terms and definitions .6 4 Principle 7 5 Test materials and apparatus .7 5.1 Biological material .7 5.2 Products and reagents 8 5.3 Apparatus .9 6 Sampling of the preservative 9 7 Test specimens . 10 7.1 Species of wood 10 7.2 Wood quality . 10 7.3 Provision
15、 of the test specimens 10 7.4 Dimensions and density of test specimens . 10 7.5 Number and distribution of test specimens 11 8 Procedure 11 8.1 Preparation of the untreated test specimens 11 8.2 Preparation of the treated test specimens . 12 8.3 Exposure to fungi . 13 8.4 Culture conditions and dura
16、tion of test . 13 8.5 Assessment of test . 13 9 Statement of results . 15 10 Test report . 15 Annex A (informative) Test fungi 17 A.1 General information on maintenance and acquisition of test strains . 17 A.2 Maintenance and treatment of test fungi . 17 A.3 Information regarding obligatory fungi 18
17、 Annex B (normative) Methods of sterilization . 20 B.1 Ionizing radiation 20 B.2 Epoxyethane-based sterilant . 20 B.3 Epoxypropane-based sterilant 21 B.4 Steam . 21 Annex C (informative) Examination of colonisation . 22 C.1 General . 22 C.2 Procedure 22 C.3 Assessment of test . 22 C.4 Validity of re
18、sults 23 DIN EN 839:2015-01 EN 839:2014 (E) 3 C.5 Statement of results 23 Annex D (informative) Example of a test report . 25 Annex E (informative) Environmental, health and safety precautions within chemical/biological laboratory . 28 Bibliography 29 DIN EN 839:2015-01 EN 839:2014 (E) 4 Foreword Th
19、is document (EN 839:2014) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement,
20、 at the latest by May 2015 and conflicting national standards shall be withdrawn at the latest by May 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or al
21、l such patent rights. This document supersedes CEN/TS 839:2008. In comparison with the previous version of the document, EN 839:2014 has been revised editorially. This document includes annexes; Annex A, Annex C, Annex D and Annex E are informative and Annex B is normative. According to the CEN-CENE
22、LEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Ic
23、eland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. DIN EN 839:2015-01 EN 839:2014 (E) 5 Introduction This European Standard specifies a laboratory method of test w
24、hich gives a basis for assessing the effectiveness of a wood preservative, when applied as a surface treatment, against wood destroying basidiomycetes. It tests whether the applied treatment is able to prevent the penetration of the fungi into the untreated interior of the test specimens under the c
25、onditions of test. This laboratory method provides one criterion by which the effectiveness of a product can be assessed. In making this assessment, the methods by which the preservative may be applied should be taken into account. It is also recommended that results from this test should be supplem
26、ented by those from other relevant tests and above all by practical experience. The procedures described in this European Standard method are intended to be carried out by suitably trained and/or supervised specialists. Suitable precautions should include the use of separate rooms, areas within room
27、s, extraction facilities, conditioning chambers and special training for personnel. Also see Annex E for environmental, health and safety precautions. DIN EN 839:2015-01 EN 839:2014 (E) 6 1 Scope This European Standard specifies a method of test for the determination of the protective effectiveness
28、of a wood preservative, applied to the surface of the wood, against wood destroying basidiomycetes cultured on an agar medium. The method is applicable to all products which are to be applied by superficial application processes. This includes: organic solvent-based wood preservatives; or organic wa
29、ter-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble products; or chemicals which are being studied as active ingredients for application by superficial processes. This method may be used in conjunction with an ageing procedure, for
30、 example EN 73. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 369
31、6, Water for analytical laboratory use - Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric av
32、erage characteristics of the total volume being sampled SOURCE: EN 1001-2, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) SOURCE: EN 1001-2, 4.83, modified 3.3 superficial application process process which does not include particular
33、features or procedures intended to overcome the natural resistance of wood to penetration of a wood preservative in its ready to use form SOURCE: EN 1001-2, 4.82 DIN EN 839:2015-01 EN 839:2014 (E) 7 4 Principle Several series of test specimens of a susceptible wood species are end-sealed with a mate
34、rial to prevent penetration of the wood preservative under test into the end grain of the test specimens. The end-sealed test specimens are treated with the wood preservative under test using the process and application rate specified by the supplier. NOTE Suitable application methods are brushing,
35、pipetting and dipping. The treated test specimens are exposed to attack by basidiomycetes in pure culture. The performance of the test product is assessed in terms of its ability to prevent decay as determined by the maximum acceptable loss in mass and the absence of visible decay of the surface and
36、 the untreated interior. 5 Test materials and apparatus 5.1 Biological material The test fungi to be used are as follows: 5.1.1 Obligatory fungus in all cases Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction o
37、f minimum 20 %. 5.1.2 Obligatory fungus for particular hazards Coriolus versicolor (Linnaeus) Qulet (CTB 863A) on hardwood and/or on softwood as appropriate. Loss in mass of beech in 16 weeks: a mass fraction of minimum 20 %. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum
38、 15 %. 5.1.3 Two species to be used compulsorily on the basis of the nature of the test product For all products except creosote-type products: Poria placenta (Fries) Cooke sensu J. Eriksson (FPRL 280) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %; Gloe
39、ophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %. For creosotes and similar products: Lentinus lepideus Fries ex Fries (BAM Ebw. 20) on softwood. Loss in mass of Scots pine sapwood in 16 weeks: a m
40、ass fraction of minimum 20 %; Lentinus cyathiformis (Schaeffer ex Fries) Bresadola (CTB 67-02B) on hardwood. Loss in mass of beech in 16 weeks: a mass fraction of minimum 20 %. DIN EN 839:2015-01 EN 839:2014 (E) 8 5.1.4 Optional fungi For specific regional uses or conditions, it is also possible to
41、select other fungi on an optional basis. When optional fungi are used, information similar to that given in Annex A for the obligatory fungi should be included in the test report. 5.1.5 Maintenance of strains The strains shall be maintained and treated (frequency of subculturing, alternation of cult
42、ure media, etc.) in accordance with the instructions of their laboratory of origin (see A.2). The parent strain shall be maintained in the laboratory of its origin so as to conserve and to ensure its vigour. If tests are not undertaken regularly or if a strain shows signs of degeneration a new stand
43、ard culture of the strain should be obtained from the laboratory of its origin for each test (see A.2). When new strains are received, the virulence shall be tested to ensure the strain can achieve the minimum loss in mass (see 5.1.1, 5.1.2 and 5.1.3). 5.2 Products and reagents 5.2.1 Culture medium
44、The culture medium is a malt agar medium with the following composition: malt extract: in concentrated form: (50 0,5) g; in powder form: (40 0,5) g; agar causing no inhibition of growth of fungi: (20 0,5) g to (30 0,5) g; water conforming to grade 3 of EN ISO 3696. quantity to make up to 1 000 ml. P
45、repare this medium by warming the mixture in a boiling water bath or steam bath, stirring until completely dissolved. Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of 3 mm to 4 mm when in its in-use position. Close the vessels as specified in 5.3
46、.1 and sterilize in an autoclave at 121 C for 20 min. Let the vessels cool in their in-use position. 5.2.2 Solvents and diluents For water soluble or water dispersible preservatives: water conforming to grade 3 of EN ISO 3696. For preservatives to be diluted or dissolved in an organic solvent: suita
47、bly volatile liquids that leave no residue in the wood that would have a toxic effect on the fungi at the end of the post-treatment conditioning period. DIN EN 839:2015-01 EN 839:2014 (E) 9 NOTE Toluene and xylene of recognized analytical grade have been found suitable. 5.2.3 Fumigant (if necessary)
48、 Xylene technical grade. 5.2.4 End-seal compound A material resistant to the penetration of the wood preservative under test and the test fungi, or separate materials for each, and without any fungistatic or fungicidal activity within the test specimen. NOTE Three brush coats of a 2-component epoxy
49、lacquer, with drying between each application, have been found to be suitable. 5.3 Apparatus 5.3.1 Culture vessels, Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml, providing a flat surface area of between 85 cm2and 120 cm2for the medium. NOTE 1 Examples of suitable vessels are given in EN 113. NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with leak proof lids, the centres of which are pierced with a round hol
